Calculation of the distribution of donor-acceptor distances in flexible bichromophoric molecules: application to intramolecular transfer of excitation energy

Valeur, Bernard; Mugnier, J.; Pouget, Jacques; Bourson, Jean; Santi, F.

doi:10.1021/j100353a027

Cited by 44 publications

(17 citation statements)

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“…Because the rate of energy transfer depends on the distance between the interacting molecules, one obvious application would be as a spectroscopic ruler. To examine the process of energy transfer, several bichromophoric da systems have been synthesized (Berberan-Santos and Valeur, 1991;Kaschke et al, 1990;Latt et al, 1965;van der Meer et al, 1994;Stryer and Haugland, 1967;Valeur, 1989;Valeur et al, 1989). The principal structure of such a system, as well as the transfer process, is illustrated in Fig.…”

Section: Introductionmentioning

confidence: 99%

Donor-Donor Energy Migration for Determining Intramolecular Distances in Proteins: I. Application of a Model to the Latent Plasminogen Activator Inhibitor-1 (PAI-1)

Karolin

Wilczyńska

et al. 1998

Biophysical Journal

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A new fluorescence spectroscopic method is presented for determining intramolecular and intermolecular distances in proteins and protein complexes, respectively. The method circumvents the general problem of achieving specific labeling with two different chromophoric molecules, as needed for the conventional donor-acceptor transfer experiments. For this, mutant forms of proteins that contain one or two unique cysteine residues can be constructed for specific labeling with one or two identical fluorescent probes, so-called donors (d). Fluorescence depolarization experiments on double-labeled Cys mutant monitor both reorientational motions of the d molecules, as well as the rate of intramolecular energy migration. In this report a model that accounts for these contributions to the fluorescence anisotropy is presented and experimentally tested. Mutants of a protease inhibitor, plasminogen activator inhibitor type-1 (PAI-1), containing one or two cysteine residues, were labeled with sulfhydryl specific derivatives of 4,4-difluoro-4-borata-3a-azonia-4a-aza-s-indacence (BODIPY). From the rate of energy migration, the intramolecular distance between the d groups was calculated by using the Forster mechanism and by accounting for the influence of local anisotropic orientation of the d molecules. The calculated intramolecular distances were compared with those obtained from the crystal structure of PAI-1 in its latent form. To test the stability of parameters extracted from experiments, synthetic data were generated and reanalyzed.

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Section: Introductionmentioning

confidence: 99%

Donor-Donor Energy Migration for Determining Intramolecular Distances in Proteins: I. Application of a Model to the Latent Plasminogen Activator Inhibitor-1 (PAI-1)

Karolin

Wilczyńska

et al. 1998

Biophysical Journal

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“…Due to flexibility of the polymethylene bridges, the dyads SQnNp can exist in many conformations with different distances between the chromophores. Calculation of the distance distribution is a separate task [63][64][65], however, we can assess the upper limit of the dyads sizes. According to calculations by PM3 method (program MOPAC2009 [66]), the length of the most elongated dyad conformer with trans-configuration of all single C-C and C-O bonds is 2.67, 2.75, 2.92, and 3.00 nm along long axis for the dyad SQnNp with n = 2, 3, 4, and 5, respectively.…”

Section: Photonics Of Styrylquinoline-naphthol Dyadsstyrylquinoline mentioning

confidence: 99%

Photonics of styrylquinoline dyads

Будыка¹

2015

Organic Photonics and Photovoltaics

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Three types of bichromophoric styrylquinoline (SQ) dyads are discussed in the review: bisstyrylquinoline dyads, styrylquinoline-merocyanine dyads, and styrylquinoline-naphthol dyads, in comparison with the corresponding model monochromophoric compounds. A variety of photochemical and photophysical processes is observed in the dyads: photoluminescence, reversible and kinetic-driven one-way photoisomerization, [2+2]photocycloaddition with formation of a single rctt-isomer of the cyclobutane derivative, Forster resonance energy transfer (FRET) where the SQ chromophore can act as an energy donor or acceptor. Operation of the dyads as photoswitches and molecular logic gates is also considered.

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“…Calculation of the distance distribution is a separate task [34][35][36], however, we can assess the upper limit of the dyads sizes. According to calculations by PM3 method (program MOPAC2009 [37]), the length of the most elongated dyad conformer with trans-configuration of all single C C and C O bonds is 2.67, 2.75, 2.92, and 3.00 nm along long axis for the dyad SQmNp with m = 2, 3, 4, and 5, respectively.…”

Section: Energy Transfermentioning

confidence: 99%

Energy transfer, fluorescence and photoisomerization of styrylquinoline–naphthol dyads with dioxypolymethylene bridges

Будыка

Sadykova

Гавришова

2012

Journal of Photochemistry and Photobiology A: Chemistry

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Calculation of the distribution of donor-acceptor distances in flexible bichromophoric molecules: application to intramolecular transfer of excitation energy

Cited by 44 publications

References 0 publications

Donor-Donor Energy Migration for Determining Intramolecular Distances in Proteins: I. Application of a Model to the Latent Plasminogen Activator Inhibitor-1 (PAI-1)

Donor-Donor Energy Migration for Determining Intramolecular Distances in Proteins: I. Application of a Model to the Latent Plasminogen Activator Inhibitor-1 (PAI-1)

Photonics of styrylquinoline dyads

Energy transfer, fluorescence and photoisomerization of styrylquinoline–naphthol dyads with dioxypolymethylene bridges

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