Calcium Waves and Development

Jaffe, Lionel F.

doi:10.1002/9780470514696.ch2

Cited by 13 publications

(9 citation statements)

References 44 publications

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“…It also functions as a patterning signal in large-scale morphogenesis [171][172][173][174][175]. It is not possible to do justice here to the enormous literature on calcium signaling, and this has been well-reviewed elsewhere [176][177][178][179]. Most of these signaling events take place through specialized receptors such as calmodulin or calcineurin [180], since calcium signals largely by virtue of its unique chemical properties-it is not a true electrical signal.…”

Section: Electroosmosismentioning

confidence: 95%

Role of Membrane Potential in the Regulation of Cell Proliferation and Differentiation

Sundelacruz

Levin

Kaplan

2009

Stem Cell Rev and Rep

414

386

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Biophysical signaling, an integral regulator of long-term cell behavior in both excitable and non-excitable cell types, offers enormous potential for modulation of important cell functions. Of particular interest to current regenerative medicine efforts, we review several examples that support the functional role of transmembrane potential (V(mem)) in the regulation of proliferation and differentiation. Interestingly, distinct V(mem) controls are found in many cancer cell and precursor cell systems, which are known for their proliferative and differentiation capacities, respectively. Collectively, the data demonstrate that bioelectric properties can serve as markers for cell characterization and can control cell mitotic activity, cell cycle progression, and differentiation. The ability to control cell functions by modulating bioelectric properties such as V(mem) would be an invaluable tool for directing stem cell behavior toward therapeutic goals. Biophysical properties of stem cells have only recently begun to be studied and are thus in need of further characterization. Understanding the molecular and mechanistic basis of biophysical regulation will point the way toward novel ways to rationally direct cell functions, allowing us to capitalize upon the potential of biophysical signaling for regenerative medicine and tissue engineering.

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Section: Electroosmosismentioning

confidence: 95%

Role of Membrane Potential in the Regulation of Cell Proliferation and Differentiation

Sundelacruz

Levin

Kaplan

2009

Stem Cell Rev and Rep

414

386

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“…These Ca 2+ signals are thought to regulate a variety of developmental events, including fertilization, cell polarization, cell division, patterning along the dorsal-ventral axis, cell migration, development of the heart, neural induction, and patterning of the brain (Jaffe, 1995;Webb and Miller, 2003;Whitaker, 2006;Parrington et al, 2007;Slusarski and Pelegri, 2007). Ca 2+ well-suited to coordinate dynamic developmental events, since Ca 2+ signals can trigger a broad spectrum of effects and can spread rapidly over long distances within an embryo.…”

Section: Introductionmentioning

confidence: 99%

Suppression of the endoplasmic reticulum calcium pump during zebrafish gastrulation affects left–right asymmetry of the heart and brain

Kreiling

Balantac

Crawford

et al. 2008

Mechanisms of Development

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Vertebrate embryos generate striking Ca(2+) patterns, which are unique regulators of dynamic developmental events. In the present study, we used zebrafish embryos as a model system to examine the developmental roles of Ca(2+) during gastrulation. We found that gastrula stage embryos maintain a distinct pattern of cytosolic Ca(2+) along the dorsal-ventral axis, with higher Ca(2+) concentrations in the ventral margin and lower Ca(2+) concentrations in the dorsal margin and dorsal forerunner cells. Suppression of the endoplasmic reticulum Ca(2+) pump with 0.5 microM thapsigargin elevates cytosolic Ca(2+) in all embryonic regions and induces a randomization of laterality in the heart and brain. Affected hearts, visualized in living embryos by a subtractive imaging technique, displayed either a reversal or loss of left-right asymmetry. Brain defects include a left-right reversal of pitx2 expression in the dorsal diencephalon and a left-right reversal of the prominent habenular nucleus in the brain. Embryos are sensitive to inhibition of the endoplasmic reticulum Ca(2+) pump during early and mid gastrulation and lose their sensitivity during late gastrulation and early segmentation. Suppression of the endoplasmic reticulum Ca(2+) pump during gastrulation inhibits expression of no tail (ntl) and left-right dynein related (lrdr) in the dorsal forerunner cells and affects development of Kupffer's vesicle, a ciliated organ that generates a counter-clockwise flow of fluid. Previous studies have shown that Ca(2+) plays a role in Kupffer's vesicle function, influencing ciliary motility and translating the vesicle's counter-clockwise flow into asymmetric patterns of gene expression. The present results suggest that Ca(2+) plays an additional role in the formation of Kupffer's vesicle.

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“…Later, the development of confocal fluorescence imaging and the increasing availability of fluorescent calcium probes defined the characteristics of the echinoderm fertilization calcium wave as we know them today: they originate at the point of sperm entry and cross the egg with a spherical wave front. Differently from ascidians and mammalian eggs (that produce repetitive calcium transients), echinoderm eggs produce one single wave after fertilization, which takes about 20 s to cross the egg (Hafner et al, 1988; Jaffe, 1995) with a constant velocity of 5-50 μm/s (Jaffe and Creton, 1998). …”

Section: Introductionmentioning

confidence: 99%

“…They can be triggered in vitro by microinjection of calcium messengers, ionophores or even by a needle prick (Jaffe, 1995; Whitaker et al, 2006). However, it is accepted that the sperm-egg interaction has a role on the triggering of a signal transduction pathway that initiates the calcium transient in vivo .…”

Section: Introductionmentioning

confidence: 99%

Calcium pathway machinery at fertilization in echinoderms

Ramos

Wessel

2013

Cell Calcium

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Calcium signaling in cells directs diverse physiological processes. The calcium waves triggered by fertilization is a highly conserved calcium signaling event essential for egg activation, and has been documented in every egg tested. This activity is one of the few highly conserved events of egg activation through the course of evolution. Echinoderm eggs, as well as many other cell types, have three main intracellular Ca2+ mobilizing messengers – IP3, cADPR and NAADP. Both cADPR and NAADP were identified as Ca2+ mobilizing messengers using the sea urchin egg homogenate, and this experimental system, along with the intact urchin and starfish oocyte/egg, continues to be a vital tool for investigating the mechanism of action of calcium signals. While many of the major regulatory steps of the IP3 pathway are well resolved, both cADPR and NAADP remain understudied in terms of our understanding of the fundamental process of egg activation at fertilization. Recently, NAADP has been shown to trigger Ca2+ release from acidic vesicles, separately from the ER, and a new class of calcium channels, the two-pore channels (TPCs), was identified as the likely targets for this messenger. Moreover, it was found that both cADPR and NAADP can be synthesized by the same family of enzymes, the ADP-rybosyl cyclases (ARCs). In this context of increasing amount of information, the potential coupling and functional roles of different messengers, intracellular stores and channels in the formation of the fertilization calcium wave in echinoderms will be critically evaluated.

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Calcium Waves and Development

Cited by 13 publications

References 44 publications

Role of Membrane Potential in the Regulation of Cell Proliferation and Differentiation

Role of Membrane Potential in the Regulation of Cell Proliferation and Differentiation

Suppression of the endoplasmic reticulum calcium pump during zebrafish gastrulation affects left–right asymmetry of the heart and brain

Calcium pathway machinery at fertilization in echinoderms

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