Calcium uptake during the cell cycle of <i>Saccharomyces cerevisiae</i>

Saavedra‐Molina, Alfredo; Villalobos, Rafael; Borbolla, Miguel

doi:10.1016/0014-5793(83)80965-x

Cited by 22 publications

(8 citation statements)

References 17 publications

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“…Thus, a rise of the biomass calcium directly after pitching into fresh broth can be due to the starvation that the cells undergo at the end of propagation or a previous fermentation. An earlier report by Saavedra-Molina et al 12 suggested that yeast cells exhibit an increased calcium uptake when they start to bud, then the uptake returns to basal values, which are maintained until the end of the cell cycle. They concluded that after the starting point of cell division, calcium must reach its minimum level to ensure continuous budding.…”

Section: Resultsmentioning

confidence: 97%

Accumulation and Release of Metal Ions by Brewer's Yeast During Successive Fermentations

Poreda¹,

Antkiewicz

Tuszyński

et al. 2009

Journal of the Institute of Brewing

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J. Inst. Brew. 115(1), 78-83, 2009Magnesium, zinc and calcium are the elements that are involved in the regulation of metabolic activity of yeast and/or other processes such as flocculation and cell division. The requirements for specific ions may be subject to changes in successive fermentations performed by the same biomass. The aim of this work was to analyze the changes in calcium, magnesium and zinc concentration in malt broth (9°P) and in the yeast over four successive fermentations. For all ionic species analysed, the qualitative uptake and release patterns observed during subsequent fermentations were similar. In the lag phase of the fermentation, yeast cells accumulated the metal ions under investigation, in order to liberate them later to an extent that depended on the specific metal and fermentation number. Some differences were noted when qualitative comparison of these processes was performed. During the first fermentation with the yeast culture, the maximum content of each ion in the yeast biomass was twoto threefold higher than in the subsequent fermentations. Calcium, magnesium and zinc levels in the biomass did not exceed 1, 6 and 0.6 mg/g yeast dry weight respectively.

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Section: Resultsmentioning

confidence: 97%

Accumulation and Release of Metal Ions by Brewer's Yeast During Successive Fermentations

Poreda¹,

Antkiewicz

Tuszyński

et al. 2009

Journal of the Institute of Brewing

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“…The first is that permeability to the drug as well as Ca2+ permeability was changed in these mutants. The second is that TFP affects the intracellular Ca2+ concentration by inactivating calmodulin or causing nonspecific damage of the membrane, There are two recent reports (Saavedra-Molina et al, 1983;Eilam, 1983) that TFP stimulates Ca2+ uptake activity in intact yeast cells. We observed that TFP also increased the calcium content of yeast cells (unpublished result).…”

Section: Discussionmentioning

confidence: 97%

Isolation and Characterization of Ca2+-sensitive Mutants of Saccharomyces cerevisiae

1986

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Thirty Ca2+-sensitive (cls: calcium sensitive) mutants of Saccharomyces cerevisiae were isolated by replica-plating. These mutants, which each had a single recessive chromosomal mutation, were divided into 18 complementation groups. Some cls mutants showed a phenotype of specific sensitivity to Ca2+, while others showed phenotypes of sensitivities to several divalent cations. From measurements of the calcium contents and initial rates of Ca2+ uptake of the cls mutants, 16 of the 18 cls complementation groups were classified into four types: type I mutants (cls5, cls6, cls13, cls14, cls15, cls16, cls17, and cls18) had both elevated calcium contents and increased uptake activities. A type II mutant (cls4) had a normal calcium content and normal uptake activity; type III mutants (cls1, cls2 and cls3) had elevated calcium contents but normal initial rates of Ca2+ uptake; type IV mutants (cls8, cls9, cls10 and cls11) had normal calcium contents but increased initial rates of Ca2+ uptake. Two of the mutants (cls7 and cls12) had intermediate biochemical properties. The primary defects of these four types of cls mutants were considered in terms of the Ca2+ transport system(s). Both type I and type III mutants, which had elevated calcium contents, simultaneously showed a trifluoperazine-sensitive phenotype, suggesting a close correlation of this phenotype with elevated calcium content. In addition, all type IV mutants were unable to utilize nonfermentable sugars. One CLS gene, CLS7, was located on the left arm of chromosome V.

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“…Therefore, a pool of Ca2+ ion or Mg2+ ion may be essential for nuclear division. Moreover, in synchronous cultures of S. cerevisiae, Ca2+ uptake was found to increase when the cells started budding and then to return to the basal value, which was maintained until the end of the first cell cycle (26). This observation suggested some unknown but crucial role of Ca2+ in the early stage of the cell cycle.…”

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confidence: 82%

Calcium-sensitive cls4 mutant of Saccharomyces cerevisiae with a defect in bud formation

Ohya¹,

Miyamoto²,

Ohsumi³

et al. 1986

J Bacteriol

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A calcium-sensitive cls4 mutant of Saccharomyces cerevisiae ceased dividing in the presence of 100 mM CaC12, producing large, round, unbudded cells. Since its DNA replication and nuclear division still continued after interruption of normal budding, the cls4 mutant had a defect in bud formation in Ca2+-rich medium. Its calcium content and calcium uptake activity were the same as those of the wild-type strain, suggesting that the primary defect of the mutation was not in a Ca2+ transport system. Genetic analysis showed that the cls4 mutation did not complement the cdc24-1 mutation, which is known to be a temperature-sensitive mutation affecting bud formation and localized cell surface growth at a restrictive temperature. Moreover, cls4 was tightly linked to cdc24, and a yeast 3.4-kilobase-pair DNA fragment carrying both the CLS4 and CDC24 genes was obtained. These results suggest that the cls4 mutation is allelic to the cdc24 mutation. Thus, Ca2+ ion seems to control bud formation and bud-localized cell surface growth.

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Calcium uptake during the cell cycle of Saccharomyces cerevisiae

Cited by 22 publications

References 17 publications

Accumulation and Release of Metal Ions by Brewer's Yeast During Successive Fermentations

Accumulation and Release of Metal Ions by Brewer's Yeast During Successive Fermentations

Isolation and Characterization of Ca2+-sensitive Mutants of Saccharomyces cerevisiae

Calcium-sensitive cls4 mutant of Saccharomyces cerevisiae with a defect in bud formation

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