Calcium uptake by reconstituted vesicles is mediated by a chymotrypsin‐sensitive peptide associated with cytochrome oxidase

Saltzgaber-Müller, J; Douglas, Michael G.; Racker, Efraim

doi:10.1016/0014-5793(80)81043-x

FEBS Letters

1980

DOI: 10.1016/0014-5793(80)81043-x

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Calcium uptake by reconstituted vesicles is mediated by a chymotrypsin‐sensitive peptide associated with cytochrome oxidase

J Saltzgaber-Müller

Michael G. Douglas

Efraim Racker

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1981

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Cited by 12 publications

References 19 publications

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Conformational coupling in H+-pumps and ATP synthesis ? its analysis with anisotropic inhibitors of energy transduction in oxidative phosphorylation

Higuti

1984

Mol Cell Biochem

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The analysis of anisotropic inhibitor-induced phenomena in mitochondria revealed that two kinds of negative charges are generated near surface of the C-side of mitochondrial inner membranes in the energized state, on the redox complexes (I, III & IV) and F0, respectively, and that positively charged anisotropic inhibitors (AI+) inhibit energy transduction in oxidative phosphorylation by binding to these negative charges. Thus, AI+ have two different inhibition sites in oxidative phosphorylation, the redox complexes and F0. The membrane components generating the negative charges in energized mitochondria were examined by the technique of photoaffinity labeling with monoazide ethidium, which is an AI+. Results showed that monoazide ethidium specifically binds to two kinds of hydrophobic protein (of 8 K and 13 K daltons) of mitochondria energized with succinate, and these proteins were named chargerin I and II, respectively. Chargerin I and II, which may be components of the redox complexes and Fo, seem to generate the negative charges described above, and these may be essential for H+-pumps in the redox complexes and F1 X F0. AI+ seem to inhibit ATP synthesis by binding to negatively charged sites of chargerin I and II. Based on these findings and the salient results on energy-transducing membranes obtained recently in other laboratories, a conformational model of H+-pumps and ATP synthesis in mitochondria is proposed, which is also applicable to ATP synthesis in other energy-transducing membranes and ATP-linked active transport of ions.

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Conformational coupling in H+-pumps and ATP synthesis ? its analysis with anisotropic inhibitors of energy transduction in oxidative phosphorylation

Higuti

1984

Mol Cell Biochem

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Some effects of dibutylchloromethyltin chloride and other reagents on mitochondrial K+ flux

Diwan

1982

J Bioenerg Biomembr

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Respiration-dependent K+ fluxes across the limiting membranes of isolated rat liver mitochondria, measured by means of 42K, are stimulated by the oxidative phosphorylation inhibitor dibutylchloromethyltin chloride (DBCT). A lack of effect of Cl- concentration indicates that the stimulation of K+ flux by DBCT is not attributable to Cl-/OH- exchange activity. The mercurial mersalyl was previously shown to stimulate respiration-dependent K+ influx. The combined presence of mersalyl plus DBCT results in a greater stimulation of K4 influx than is caused by either DBCT or mersalyl alone. The oxidative phosphorylation inhibitor oligomycin, which alone has no effect on respiration-dependent K+ influx, enhances the stimulatory effect of mersalyl on K+ influx. The data are consistent with, although not proof of, a direct interaction of the K+ transport mechanism with the mitochondrial energy transduction apparatus.

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Calcium transport sensitive to ruthenium red in cytochrome oxidase vesicles reconstituted with mitochondrial proteins

Zazueta

Holguín

Ramı́rez

1991

J Bioenerg Biomembr

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We describe a calcium transport that is sensitive to ruthenium red in liposomes reconstituted with mitochondrial extracts. This system is able to build an internally negative membrane potential, which allows the electrogenic influx of Ca2+ and Sr2+. Proteins with molecular weights higher than 35 kDa were incorporated to the vesicles, and enhanced the accumulation of the cation in an energy-dependent fashion.

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Calcium uptake by reconstituted vesicles is mediated by a chymotrypsin‐sensitive peptide associated with cytochrome oxidase

Cited by 12 publications

References 19 publications

Conformational coupling in H+-pumps and ATP synthesis ? its analysis with anisotropic inhibitors of energy transduction in oxidative phosphorylation

Conformational coupling in H+-pumps and ATP synthesis ? its analysis with anisotropic inhibitors of energy transduction in oxidative phosphorylation

Some effects of dibutylchloromethyltin chloride and other reagents on mitochondrial K+ flux

Calcium transport sensitive to ruthenium red in cytochrome oxidase vesicles reconstituted with mitochondrial proteins

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