Calcium Uncaging with Visible Light

Agarwal, Hitesh K.; Janíček, Radoslav; Chi, San‐Hui; Perry, Joseph W.; Niggli, Ernst; Ellis‐Davies, Graham C. R.

doi:10.1021/jacs.5b11606

Cited by 71 publications

(85 citation statements)

References 59 publications

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“…[58] Recently, the calcium chelator 19 was developed and was activated with 810 nm (two-photon) or 405 nm light (Figure 13a). [59] When cardiac myocytes bearing a fluorescent Ca 2+ reporter were treated with 19 , no change in calcium levels was observed. However, upon illumination, a calcium wave was detected in both directions from the light source (Figure 13b,c).…”

Section: Optical Control Of Small Moleculesmentioning

confidence: 99%

Optochemical Control of Biological Processes in Cells and Animals

et al. 2018

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Biological processes are naturally regulated with high spatial and temporal control, as is perhaps most evident in metazoan embryogenesis. Chemical tools have been extensively utilized in cell and developmental biology to investigate cellular processes, and conditional control methods have expanded applications of these technologies toward resolving complex biological questions. Light represents an excellent external trigger since it can be controlled with very high spatial and temporal precision. To this end, several optically regulated tools have been developed and applied to living systems. In this review we discuss recent developments of optochemical tools, including small molecules, peptides, proteins, and nucleic acids that can be irreversibly or reversibly controlled through light irradiation, with a focus on applications in cells and animals.

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Section: Optical Control Of Small Moleculesmentioning

confidence: 99%

Optochemical Control of Biological Processes in Cells and Animals

et al. 2018

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“…They can be used for physiological processes such as Ca 2+ signaling with good spatiotemporal control. 69,226 …”

Section: Other Applications Of Light In Ddssmentioning

confidence: 99%

Smart Nanostructures for Cargo Delivery: Uncaging and Activating by Light

et al. 2017

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Nanotechnology has begun to play a remarkable role in various fields of science and technology. In biomedical applications, nanoparticles have opened new horizons, especially for biosensing, targeted delivery of therapeutics, and so forth. Among drug delivery systems (DDSs), smart nanocarriers that respond to specific stimuli in their environment represent a growing field. Nanoplatforms that can be activated by an external application of light can be used for a wide variety of photoactivated therapies, especially light-triggered DDSs, relying on photoisomerization, photo-cross-linking/un-cross-linking, photoreduction, and so forth. In addition, light activation has potential in photodynamic therapy, photothermal therapy, radiotherapy, protected delivery of bioactive moieties, anticancer drug delivery systems, and theranostics (i.e., real-time monitoring and tracking combined with a therapeutic action to different diseases sites and organs). Combinations of these approaches can lead to enhanced and synergistic therapies, employing light as a trigger or for activation. Nonlinear light absorption mechanisms such as two-photon absorption and photon upconversion have been employed in the design of light-responsive DDSs. The integration of a light stimulus into dual/multiresponsive nanocarriers can provide spatiotemporal controlled delivery and release of therapeutic agents, targeted and controlled nanosystems, combined delivery of two or more agents, their on-demand release under specific conditions, and so forth. Overall, light-activated nanomedicines and DDSs are expected to provide more effective therapies against serious diseases such as cancers, inflammation, infections, and cardiovascular disease with reduced side effects and will open new doors toward the treatment of patients worldwide.

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“…Since then, photocages have proven useful tools for various biological applications . Recent examples include local activation of antimiR‐92a in living mice, which improves wound healing, manipulation of a protein function in living zebrafish embryos, and induction of calcium signaling events in mouse cardiac myocytes . To utilize biologically acceptable and penetrable irradiation for complex scenarios of light control, further development of efficient, visible‐light‐absorbing photocages is desired.…”

Section: Introductionmentioning

confidence: 99%

A New Photocage Derived from Fluorene

Reinfelds

Cosel

Falahati

et al. 2018

Chemistry A European J

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Photolabile protecting groups (PPGs or photocages) are increasingly subject to molecular design to meet requirements such as absorbance in the visible spectral range, high molar absorption coefficients, and high quantum yields of leaving group release. Improvements in these properties for the promising 3-diethylaminobenzyl (DEAMb) photocage, the photoactivity of which is based on the Zimmerman meta effect, are reported. Expansion of the aromatic system with a second aromatic ring resulted in improved spectral properties. A systematic trend relating the electronic (π-donor or acceptor) properties of the new aryl substituent and its position in the DEAMb ring to changes in the spectral properties could be observed. Conclusions from the experimental results were supported by computations obtained by using time-dependent DFT. A second generation of DEAMb-based photocages was designed. A rigid linker was introduced to ensure more efficient conjugation of the aromatic ring π systems by limiting rotational freedom. The resulting fluorenol (9-hydroxyfluorene)-based photocages had superior spectral properties to those of simple biphenyl systems. The best uncaging cross section achieved was 5320 m cm (ϵΦ ).

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Calcium Uncaging with Visible Light

Cited by 71 publications

References 59 publications

Optochemical Control of Biological Processes in Cells and Animals

Optochemical Control of Biological Processes in Cells and Animals

Smart Nanostructures for Cargo Delivery: Uncaging and Activating by Light

A New Photocage Derived from Fluorene

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