Calcium signatures and signaling in cytosol and organelles of tobacco cells induced by plant defense elicitors

Manzoor, Hamid; Chiltz, Annick; Madani, Siham; Vatsa, Parul; Schoefs, Benoı̂t; Pugin, Alain; Garcia-Brugger, Angela

doi:10.1016/j.ceca.2012.02.006

Cited by 51 publications

(50 citation statements)

References 68 publications

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“…This is in agreement with early work based on aequorin and rhod2 measurements in Arabidopsis seedlings and maize suspensioncultured cells (Subbaiah et al, 1998;Logan and Knight, 2003). By contrast, higher intracellular Ca 2+ concentrations, with less clearly defined subcellular differences, have been reported from tobacco suspension cells (Manzoor et al, 2012). Our observations further refine the understanding of the relationship of Ca 2+ dynamics in the cytosol and the mitochondrial matrix.…”

Section: Toward An Understanding Of Subcellular Ca 2+ Dynamicssupporting

confidence: 81%

“…that, similar to mammalian systems, an increased Ca 2+ concentration in the matrix can coincide with stimulated mitochondrial respiration in plant cells (Manzoor et al, 2012). In addition, stressinducible membrane potential pulses in single Arabidopsis mitochondria have been associated with mitochondrial Ca 2+ dynamics.…”

Section: Phenotypes and The Regulatory Impact Of Micu In The Context mentioning

confidence: 98%

“…Increased mitochondrial DNA copy numbers have been associated with dysfunction-induced mitochondrial biogenesis in animals. Tubular, as opposed to saccular cristae, which are typical for root mitochondria, suggest limited bioenergetic activity, consistent with ongoing synthesis of the respiratory machinery and/or acclimation to respiratory induction by constitutive elevation of matrix Ca 2+ (Manzoor et al, 2012).…”

Section: Phenotypes and The Regulatory Impact Of Micu In The Context mentioning

confidence: 99%

“…Furthermore, the EF-hand protein LETM1 and the calmodulin-like protein CML30 have recently been found to be localized in mitochondria, and additional proteins with Ca 2+ binding motifs have been detected in proteomic data sets of plant mitochondria (Chigri et al, 2012;Zhang et al, 2012;Salvato et al, 2014). Moreover, there is evidence for Ca 2+ dependence of mitochondrial permeability transition, protein import, and respiration in plants (Miernyk et al, 1987;Arpagaus et al, 2002;Kuhn et al, 2009;Manzoor et al, 2012). Nevertheless, it remains unclear if metabolic flux through pyruvate dehydrogenase and the tricarboxylic acid cycle can be regulated by Ca 2+ in vivo (Møller and Rasmusson, 1998).…”

Section: Introductionmentioning

confidence: 99%

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The EF-Hand Ca²⁺ Binding Protein MICU Choreographs Mitochondrial Ca²⁺ Dynamics in Arabidopsis

et al. 2015

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Plant organelle function must constantly adjust to environmental conditions, which requires dynamic coordination. Ca 2+ signaling may play a central role in this process. Free Ca 2+ dynamics are tightly regulated and differ markedly between the cytosol, plastid stroma, and mitochondrial matrix. The mechanistic basis of compartment-specific Ca 2+ dynamics is poorly understood. Here, we studied the function of At-MICU, an EF-hand protein of Arabidopsis thaliana with homology to constituents of the mitochondrial Ca 2+ uniporter machinery in mammals. MICU binds Ca 2+ and localizes to the mitochondria in Arabidopsis. In vivo imaging of roots expressing a genetically encoded Ca 2+ sensor in the mitochondrial matrix revealed that lack of MICU increased resting concentrations of free Ca 2+ in the matrix. Furthermore, Ca 2+ elevations triggered by auxin and extracellular ATP occurred more rapidly and reached higher maximal concentrations in the mitochondria of micu mutants, whereas cytosolic Ca 2+ signatures remained unchanged. These findings support the idea that a conserved uniporter system, with composition and regulation distinct from the mammalian machinery, mediates mitochondrial Ca 2+ uptake in plants under in vivo conditions. They further suggest that MICU acts as a throttle that controls Ca 2+ uptake by moderating influx, thereby shaping Ca 2+ signatures in the matrix and preserving mitochondrial homeostasis. Our results open the door to genetic dissection of mitochondrial Ca 2+ signaling in plants.

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Section: Toward An Understanding Of Subcellular Ca 2+ Dynamicssupporting

confidence: 81%

Section: Phenotypes and The Regulatory Impact Of Micu In The Context mentioning

confidence: 98%

Section: Phenotypes and The Regulatory Impact Of Micu In The Context mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The EF-Hand Ca²⁺ Binding Protein MICU Choreographs Mitochondrial Ca²⁺ Dynamics in Arabidopsis

et al. 2015

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“…Calcium ions are very important second messengers in plants and its level in intracellular compartments is determinant for the kind of physiological response. In tobacco cells OGAs induced different patterns of Ca 2+ influx into cytosol, mitochondria and chloroplasts [54]. The increase in cytosolic free Ca 2+ has been proposed to mediate the regulation of stomatal aperture and production of hydrogen peroxide in the guard cells of tomato (Licopersicon esculentum L.) and Commelina communis L. [55].…”

Section: Biological Activity Pd Organism Referencementioning

confidence: 99%

Plant Cell Wall Polymers: Function, Structure and Biological Activity of Their Derivatives

et al. 2012

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Extracellular Alkalinization Assay for the Detection of Early Defense Response

2017

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Plant recognition of invading organisms occurs through identification of foreign molecules associated with attackers and of self‐derived, damage‐associated molecules. Perception of these molecules activates signaling processes including dynamic changes in ion balance, production of second messengers such as reactive oxygen species and nitric oxide, increased levels of plant hormones, and map kinase cascade activation. Together these signaling events stimulate transcriptional changes to initiate plant defense responses. Among the earliest detectable signaling events is a rapid increase in apoplastic pH, i.e., extracellular alkalinization. Here, an assay for quantification of this alkalinization response using suspension‐cultured cell lines for Arabidopsis, potato, and maize is described. This assay is an inexpensive, fast, simple, and reproducible method to quantify defense signaling output, providing a powerful tool for evaluating early plant responses to elicitors and pathogens. Results from the alkalinization assay are comparable to other more costly and time‐consuming methods for assessing defense signaling, such as measurement of the oxidative burst, calcium influx, and marker gene expression. This bioassay is a quantitative and robust method for evaluation of plant defense output. © 2017 by John Wiley & Sons, Inc.

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Calcium signatures and signaling in cytosol and organelles of tobacco cells induced by plant defense elicitors

Cited by 51 publications

References 68 publications

The EF-Hand Ca²⁺ Binding Protein MICU Choreographs Mitochondrial Ca²⁺ Dynamics in Arabidopsis

The EF-Hand Ca²⁺ Binding Protein MICU Choreographs Mitochondrial Ca²⁺ Dynamics in Arabidopsis

Plant Cell Wall Polymers: Function, Structure and Biological Activity of Their Derivatives

Extracellular Alkalinization Assay for the Detection of Early Defense Response

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Calcium signatures and signaling in cytosol and organelles of tobacco cells induced by plant defense elicitors

Cited by 51 publications

References 68 publications

The EF-Hand Ca2+ Binding Protein MICU Choreographs Mitochondrial Ca2+ Dynamics in Arabidopsis

The EF-Hand Ca2+ Binding Protein MICU Choreographs Mitochondrial Ca2+ Dynamics in Arabidopsis

Plant Cell Wall Polymers: Function, Structure and Biological Activity of Their Derivatives

Extracellular Alkalinization Assay for the Detection of Early Defense Response

Contact Info

Product

Resources

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The EF-Hand Ca²⁺ Binding Protein MICU Choreographs Mitochondrial Ca²⁺ Dynamics in Arabidopsis

The EF-Hand Ca²⁺ Binding Protein MICU Choreographs Mitochondrial Ca²⁺ Dynamics in Arabidopsis