Calcium signaling surrounding fertilization in the nematode Caenorhabditis elegans

Singaravelu, Gunasekaran; Singson, Andrew

doi:10.1016/j.ceca.2012.11.009

Cited by 21 publications

(13 citation statements)

References 53 publications

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“…Basal Ca levels are higher at 48 h than in L1 worms. This is consistent with the need for Ca during fertilization (Singaravelu and Singson 2013). …”

Section: Discussionsupporting

confidence: 89%

Role of Caenorhabditis elegans AKT-1/2 and SGK-1 in Manganese Toxicity

et al. 2018

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Excessive levels of the essential metal manganese (Mn) may cause a syndrome similar to Parkinson's disease. The model organism Caenorhabditis elegans mimics some of Mn effects in mammals, including dopaminergic neurodegeneration, oxidative stress, and increased levels of AKT. The evolutionarily conserved insulin/insulin-like growth factor-1 signaling pathway (IIS) modulates worm longevity, metabolism, and antioxidant responses by antagonizing the transcription factors DAF-16/FOXO and SKN-1/Nrf-2. AKT-1, AKT-2, and SGK-1 act upstream of these transcription factors. To study the role of these proteins in C. elegans response to Mn intoxication, wild-type N2 and loss-of-function mutants were exposed to Mn (2.5 to 100 mM) for 1 h at the L1 larval stage. Strains with loss-of-function in akt-1, akt-2, and sgk-1 had higher resistance to Mn compared to N2 in the survival test. All strains tested accumulated Mn similarly, as shown by ICP-MS. DAF-16 nuclear translocation was observed by fluorescence microscopy in WT and loss-of-function strains exposed to Mn. qRT-PCR data indicate increased expression of γ-glutamyl cysteine synthetase (GCS-1) antioxidant enzyme in akt-1 mutants. The expression of sod-3 (superoxide dismutase homologue) was increased in the akt-1 mutant worms, independent of Mn treatment. However, dopaminergic neurons degenerated even in the more resistant strains. Dopaminergic function was evaluated with the basal slowing response behavioral test and dopaminergic neuron integrity was evaluated using worms expressing green fluorescent protein (GFP) under the dopamine transporter (DAT-1) promoter. These results suggest that AKT-1/2 and SGK-1 play a role in C. elegans response to Mn intoxication. However, tissue-specific responses may occur in dopaminergic neurons, contributing to degeneration.

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“…Basal Ca levels are higher at 48 h than in L1 worms. This is consistent with the need for Ca during fertilization (Singaravelu and Singson 2013). …”

Section: Discussionsupporting

confidence: 89%

Role of Caenorhabditis elegans AKT-1/2 and SGK-1 in Manganese Toxicity

et al. 2018

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“…Similar ndings (reduced fecundity by IVM treatment) were also obtained in sheep (Cooperia curticei) [24], cattle (Cooperia curticei) [25] and pig (Oesophagostomum dentatum) [16] nematodes. A recent study in the free-living nematode Caenorhabditis elegans showed that calcium signaling plays a crucial role in fertilization [26]. Interestingly, another study in larial human parasitic nematode Brugia malayi reported that some genes in the Ca 2+ signaling pathway and a number of other genes involved in fertilization were down-regulated following exposure to IVM, which caused adverse effects on ovulation [27].…”

Section: Discussionmentioning

confidence: 99%

First detection and evaluation of ivermectin resistance in Haemonchus contortus from sheep in Zhaosu, Xinjiang, China

Tuersong¹,

Yan²,

Zhou³

et al. 2020

Preprint

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Background: In 2017 in Zhaosu, Xinjiang, China, a series of sheep death suspected to have reduced the potency of ivermectin (IVM) have raised the vigilance of herdsmen. At the same time, veterinary practitioners warned of the potential risk of the lack of efficacy of IVM in the treatment of Haemonchus contortus. Methods: In this study, the efficacy of IVM against H. contortus was assessed under controlled experimental conditions by using larval developmental assay (LDA) and faecal egg count reduction test (FECRT) to determine whether the reduced efficacy in sheep in Zhaosu was caused by IVM resistance. Results: In total, 1560 adult worms were isolated from the abomasum of post mortem examination of dead sheep which received recent IVM (0.2 mg/kg) treatment before death and eggs were collected from female worms for usage in LDA. The LDA result revealed that significant difference in resistance ratio (RR) was detected between the IVM-susceptible and -resistant isolates (6.09 for Zhaosu strain) of H. contortus. The Zhaosu strain was maintained in laboratory for FECRT, which showed that the efficacy of IVM was 0% at 1, 2 and 3 times dose, but at 4.8 times dose, it reached 87.5% at 14 days post-treatment. Conclusion: The findings suggested that controlling H. contortus of small ruminants in Zhaosu requires an integrated parasite management strategy that should include monitoring of infection and anthelmintic resistance, and increasing the awareness of herdsmen on the importance of using the appropriate drug usage to improve nematode control in sheep.

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“…In addition, the iron channel is also a kind of factor affecting sperm activation. It was reported that triazole fungicides can non-specifically inhibit calcium channels and disturb intracellular Ca 2+ [43,44]. All of these may be mechanisms by which epoxiconazole causes abnormal sperm activation.…”

Section: Discussionmentioning

confidence: 99%

The Use of the Nematode Caenorhabditis elegans to Evaluate the Adverse Effects of Epoxiconazole Exposure on Spermatogenesis

Zhang

et al. 2016

IJERPH

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There is increasing evidence that epoxiconazole exposure can affect reproductive function, but few studies have investigated adverse effects on spermatogenesis. The nematode Caenorhabditis elegans (C. elegans) was used in our study to assess effects of epoxiconazole on spermatogenesis in male nematodes after 48 h of exposure to concentrations of 0.1, 1.0, or 10.0 μg/L. The results demonstrated that epoxiconazole exposure affected spermatogenesis, decreasing the number of total germ cells, mitotic cells, meiotic cells and spermatids, spermatid diameter, and cross-sectional area, and inducing mitotic germ cell proliferation arrest, premature entry into meiosis, and sperm activation inhibition; however, sperm transfer showed no abnormal changes. In addition, the results showed that epoxiconazole activated the transforming growth factor-β (TGFβ) signaling pathway and increased the expression levels of gene daf-1, daf-3, daf-4, daf-5 and daf-7 in nematodes. We therefore propose that epoxiconazole acts by activating the TGFβ signaling pathway, leading to the impairment of spermatogenesis and the consequent decline in male fertility.

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Calcium signaling surrounding fertilization in the nematode Caenorhabditis elegans

Cited by 21 publications

References 53 publications

Role of Caenorhabditis elegans AKT-1/2 and SGK-1 in Manganese Toxicity

Role of Caenorhabditis elegans AKT-1/2 and SGK-1 in Manganese Toxicity

First detection and evaluation of ivermectin resistance in Haemonchus contortus from sheep in Zhaosu, Xinjiang, China

The Use of the Nematode Caenorhabditis elegans to Evaluate the Adverse Effects of Epoxiconazole Exposure on Spermatogenesis

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