Calcium signaling in prostate cancer cells: Evidence for multiple receptors and enhanced sensitivity to bombesin/GRP

Wasilenko, William J.; Cooper, Joel; Palad, Ann J.; Somers, Kenneth D.; Blackmore, Peter F.; Rhim, Johng S.; Wright, George L.; Schellhammer, Paul F.

doi:10.1002/(sici)1097-0045(19970215)30:3<167::aid-pros4>3.0.co;2-j

Cited by 52 publications

(30 citation statements)

References 38 publications

(43 reference statements)

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“…41 Some of the other peptides having effects in human prostate include oxytocin and vasopressin. 42 Although purinoceptor mechanisms have been investigated briefly in human prostate tissue, 43 their role in human prostatic contractility is yet to be determined. In rats and guinea-pigs, post-junctional P2 and prejunctional adenosine receptors modulate contractility to varying degrees at different frequencies in different species.…”

Section: Animal Prostate Models Of Human Prostate Contractilitymentioning

confidence: 56%

Current models of human prostate contractility

Haynes

Ventura

2005

Clin Exp Pharma Physio

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SUMMARY1. The human prostate is a compact gland contributing to seminal fluid. With increasing age, most humans will develop benign prostatic hyperplasia, a condition of prostatic enlargement and contractility that leads to occlusion of the urethra. Over many years, investigators have used a variety of animal and cell culture models to elucidate some of the contractile and proliferative mechanisms that may be associated with the development of this condition.2. This review briefly assesses the current state of knowledge of the mechanisms underlying human prostatic contractility and compares it with that of animal and cell culture models. It is not intended as a comprehensive methodological review, nor is it intended to indicate our preferences for either model. Our aim is to correlate findings from animal and cell culture models with the current understanding of human prostate contractility.3. We hope that the present review will increase awareness of the suitability of the current models in developing our understanding of benign prostatic hyperplasia.

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Section: Animal Prostate Models Of Human Prostate Contractilitymentioning

confidence: 56%

Current models of human prostate contractility

Haynes

Ventura

2005

Clin Exp Pharma Physio

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“…However, we did not measure an increase in intracellular calcium levels following bFGF treatment. Interestingly, Wasilenko and associates recently reported that neither of the calcium agonists tested was able to induce an elevation of intracellular calcium in LNCaP cells (Wasilenko et al, 1997). Thus, other signalling pathways must be considered for inhibition of AR expression by bFGF.…”

Section: Discussionmentioning

confidence: 99%

Androgen receptor protein is down-regulated by basic fibroblast growth factor in prostate cancer cells

et al. 2000

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Interactions between polypeptide growth factors and the androgen receptor (AR) are important for regulation of cellular events in carcinoma of the prostate. Basic fibroblast growth factor (bFGF), the prototype of heparin-binding growth factors, and the AR are commonly expressed in prostate cancer. bFGF diminished prostate-specific antigen protein in the supernatants of androgen-stimulated human prostate cancer cells LNCaP by 80%. In the present study, we asked whether the bFGF effect on prostate-specific antigen is preceded by action on AR expression. LNCaP cells were treated with bFGF and AR protein expression was determined by immunoblotting and ligand binding assay. bFGF down-regulated AR protein in a dose-dependent manner showing a maximal effect at 50 ng ml −1 both in the presence or absence of dihydrotestosterone. Down-regulation of AR protein expression occurred already after 8 h of bFGF treatment and a maximal inhibition was observed 24 h after addition of bFGF to culture media. As AR expression can be reduced by an increase in intracellular calcium levels, we investigated whether the bFGF effect on AR protein is mediated by this mechanism. Calcium release from intracellular stores and store-operated calcium influx after treatment with either bFGF or calcium ionophore A 23187 were measured by single cell fluorescence technique. The ionophore A 23187 was able to induce calcium influx and an increase in cytoplasmic calcium concentration in LNCaP cells. In contrast, bFGF was incapable of eliciting a similar effect. In contrast to AR protein, AR mRNA levels were not affected by bFGF as shown by semiquantitative reverse transcription polymerase chain reaction. In summary, these studies show that bFGF is a potent negative regulator of AR protein expression in the human prostate cancer cell line LNCaP. © 2000 Cancer Research Campaign

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“…Multiple P1 and P2 receptors are expressed by prostate cancer cells [78,180,363,428]. For a detailed coverage of the involvement of purinergic signalling in malignant prostatic hyperplasia see [62].…”

Section: Prostatementioning

confidence: 99%

Purinergic signalling in the reproductive system in health and disease

Burnstock

2013

Purinergic Signalling

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There are multiple roles for purinergic signalling in both male and female reproductive organs. ATP, released as a cotransmitter with noradrenaline from sympathetic nerves, contracts smooth muscle via P2X1 receptors in vas deferens, seminal vesicles, prostate and uterus, as well as in blood vessels. Male infertility occurs in P2X1 receptor knockout mice. Both short-and long-term trophic purinergic signalling occurs in reproductive organs. Purinergic signalling is involved in hormone secretion, penile erection, sperm motility and capacitation, and mucous production. Changes in purinoceptor expression occur in pathophysiological conditions, including pre-eclampsia, cancer and pain.

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Calcium signaling in prostate cancer cells: Evidence for multiple receptors and enhanced sensitivity to bombesin/GRP

Cited by 52 publications

References 38 publications

Current models of human prostate contractility

Current models of human prostate contractility

Androgen receptor protein is down-regulated by basic fibroblast growth factor in prostate cancer cells

Purinergic signalling in the reproductive system in health and disease

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