Calcium-Sensitive Particulate Guanylyl Cyclase as a Modulator of cAMP in Olfactory Receptor Neurons

Moon, Cheil; Jaberi, Parham; Otto‐Bruc, Annie; Baehr, Wolfgang; Palczewski, Krzysztof; Ronnett, Gabriele V.

doi:10.1523/jneurosci.18-09-03195.1998

Cited by 62 publications

(92 citation statements)

References 83 publications

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“…Although GC-D is expressed exclusively in the CO 2 -responsive OSNs, other membrane GCs and soluble (s)GCs are known to function in the olfactory epithelium (23). We tested whether several other mammalian membrane GCs could be activated by bicarbonate.…”

Section: Resultsmentioning

confidence: 99%

Guanylyl cyclase-D in the olfactory CO ₂ neurons is activated by bicarbonate

Sun

Wang

Jian

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

113

110

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Atmospheric CO2 is an important environmental cue that regulates several types of animal behavior. In mice, CO 2 responses of the olfactory sensory neurons (OSNs) require the activity of carbonic anhydrase to catalyze the conversion of CO 2 to bicarbonate and the opening of cGMP-sensitive ion channels. However, it remains unknown how the enhancement of bicarbonate levels results in cGMP production. Here, we show that bicarbonate activates cGMP-producing ability of guanylyl cyclase-D (GC-D), a membrane GC exclusively expressed in the CO 2-responsive OSNs, by directly acting on the intracellular cyclase domain of GC-D. Also, the molecular mechanism for GC-D activation is distinct from the commonly believed model of ''release from repression'' for other membrane GCs. Our results contribute to our understanding of the molecular mechanisms of CO 2 sensing and suggest diverse mechanisms of molecular activation among membrane GCs.carbonic anhydrase ͉ CNG channels ͉ necklace glomeruli ͉ transduction

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Section: Resultsmentioning

confidence: 99%

Guanylyl cyclase-D in the olfactory CO ₂ neurons is activated by bicarbonate

Sun

Wang

Jian

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

113

110

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“…PKG serine/threonine kinases have numerous targets that could affect neuronal function and growth, such as ion channels (Stockand and Sansom 1996;Carrier et al 1997;Taguchi et al 1997;Alioua et al 1998;Han et al 1998;Vaandrager et al 1998;Wexler et al 1998), ATPases (e.g., Uneyama et al 1998), and regulators of gene expression (Gudi et al 1997;Idriss et al 1999). PKG may interact with other second messenger systems such as PKA, either by regulating such other systems (Moon et al 1998) or by phosphorylating common targets (Lengyel et al 1999). It is interesting that mutations of dunce that increase cAMP abundance lead to more rapid stimulus-dependent response decrement (Engel and Wu 1996), opposite to the effect of increased PKG activity in foraging rover genotypes.…”

Section: Pkg Affects Response Modification In Drosophilamentioning

confidence: 99%

“…Interest in the cGMP-dependent serine/threonine kinase, or PKG, has grown with the awareness of the diversity of biochemical pathways that involve cGMP (Koesling et al 1991;Garbers 1992;Sheth et al 1997;Wang and Robinson 1997;Moon et al 1998;Simpson et al 1999). PKG has been shown to influence characteristics involved in both functional and developmental plasticity of neural circuits (Zhuo et al 1994(Zhuo et al , 1999Lev-Ram et al 1997;Wu et al 1998b;Calabresi et al 1999;Lewin and Walters 1999;Renger et al 1999;Yawo 1999).…”

mentioning

confidence: 99%

A cGMP-Dependent Protein Kinase Gene, foraging, Modifies Habituation-Like Response Decrement of the Giant Fiber Escape Circuit in Drosophila

Engel¹,

Xie²,

Sokolowski³

et al. 2000

Learn. Mem.

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The Drosophila giant fiber jump-and-flight escape response is a model for genetic analysis of both the physiology and the plasticity of a sensorimotor behavioral pathway. We previously established the electrically induced giant fiber response in intact tethered flies as a model for habituation, a form of nonassociative learning. Here, we show that the rate of stimulus-dependent response decrement of this neural pathway in a habituation protocol is correlated with PKG (cGMP-Dependent Protein Kinase) activity and foraging behavior. We assayed response decrement for natural and mutant rover and sitter alleles of the foraging (for) gene that encodes a Drosophila PKG. Rover larvae and adults, which have higher PKG activities, travel significantly farther while foraging than sitters with lower PKG activities. Response decrement was most rapid in genotypes previously shown to have low PKG activities and sitter-like foraging behavior. We also found differences in spontaneous recovery (the reversal of response decrement during a rest from stimulation) and a dishabituation-like phenomenon (the reversal of response decrement evoked by a novel stimulus). This electrophysiological study in an intact animal preparation provides one of the first direct demonstrations that PKG can affect plasticity in a simple learning paradigm. It increases our understanding of the complex interplay of factors that can modulate the sensitivity of the giant fiber escape response, and it defines a new adult-stage phenotype of the foraging locus. Finally, these results show that behaviorally relevant neural plasticity in an identified circuit can be influenced by a single-locus genetic polymorphism existing in a natural population of Drosophila.Protein kinases play key roles in the activity-dependent modulation of neuronal activity and morphology. Interest in the cGMP-dependent serine/threonine kinase, or PKG, has grown with the awareness of the diversity of biochemical pathways that involve cGMP (Koesling et al.

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“…Immunoblot analysis was performed using retinal extract preparations to assess expression levels of Bcl-2 as previously reported with some modifications (Moon et al, 1998). Retinal extracts were prepared by homogenizing a single retina in 100 ul of PBS.…”

Section: Immunoblot Analysismentioning

confidence: 99%

Differential Cell Death and Bcl-2 Expression in Mouse Retina after Glutathione Decrease by Systemic D,L-Buthionine Sulphoximine Administration

Park

Kim

Moon

et al. 2013

Molecules and Cells

Self Cite

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Glutathione (GSH) plays a critical role in cellular defense against unregulated oxidative stress in mammalian cells including neurons. We previously demonstrated that GSH decrease using [D, L]-buthionine sulphoximine (BSO) induces retinal cell death, but the underlying mechanisms of this are still unclear. Here, we demonstrated that retinal GSH level is closely related to retinal cell death as well as expression of an anti-apoptotic molecule, Bcl-2, in the retina. We induced differential expression of retinal GSH by single and multiple administrations of BSO, and examined retinal GSH levels and retinal cell death in vivo. Single BSO administration showed a transient decrease in the retinal GSH level, whereas multiple BSO administration showed a persistent decrease in the retinal GSH level. Retinal cell death also showed similar patterns: transient increase of retinal cell death was observed after single BSO administration, whereas persistent increase of retinal cell death was observed after multiple BSO administration. Changes in the retinal GSH level affected Bcl-2 expression in the retina. Immunoblot and immunohistochemical analyses showed that single and multiple administration of BSO induced differential expressions of Bcl-2 in the retina. Taken together, the results of our study suggest that the retinal GSH is important for the survival of retinal cells, and retinal GSH appears to be deeply related to Bcl-2 expression in the retina. Thus, alteration of Bcl-2 expression may provide a therapeutic tool for retinal degenerative diseases caused by retinal oxidative stress such as glaucoma or retinopathy.

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Calcium-Sensitive Particulate Guanylyl Cyclase as a Modulator of cAMP in Olfactory Receptor Neurons

Cited by 62 publications

References 83 publications

Guanylyl cyclase-D in the olfactory CO ₂ neurons is activated by bicarbonate

Guanylyl cyclase-D in the olfactory CO ₂ neurons is activated by bicarbonate

A cGMP-Dependent Protein Kinase Gene, foraging, Modifies Habituation-Like Response Decrement of the Giant Fiber Escape Circuit in Drosophila

Differential Cell Death and Bcl-2 Expression in Mouse Retina after Glutathione Decrease by Systemic D,L-Buthionine Sulphoximine Administration

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Calcium-Sensitive Particulate Guanylyl Cyclase as a Modulator of cAMP in Olfactory Receptor Neurons

Cited by 62 publications

References 83 publications

Guanylyl cyclase-D in the olfactory CO 2 neurons is activated by bicarbonate

Guanylyl cyclase-D in the olfactory CO 2 neurons is activated by bicarbonate

A cGMP-Dependent Protein Kinase Gene, foraging, Modifies Habituation-Like Response Decrement of the Giant Fiber Escape Circuit in Drosophila

Differential Cell Death and Bcl-2 Expression in Mouse Retina after Glutathione Decrease by Systemic D,L-Buthionine Sulphoximine Administration

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Guanylyl cyclase-D in the olfactory CO ₂ neurons is activated by bicarbonate

Guanylyl cyclase-D in the olfactory CO ₂ neurons is activated by bicarbonate