Calcium-sensing receptor-mediated activation of phospholipase C-γ1 is downstream of phospholipase C-β and protein kinase C in MC3T3-E1 osteoblasts

The extracellular calcium sensing receptor is expressed in mouse mesangial cells and modulates cell proliferation

“…This value showed to be slightly higher than the previously reported values. Bai et al (Bai et al,1998) and Godwin (Godwin and Soltoff, 2002) demonstrated values of 4.0 mM in the CaSR transfected kidney cells and 4.6 mM in the MC3T3-E1 osteoblasts, respectively.…”

Extracellular Calcium as a Candidate Mediator of Prostate Cancer Skeletal Metastasis

“…5B). Pertussis toxin, a G-protein inhibitor that restricts CaSR signaling (39,40), effectively decreased the attachment of (Fig. 6B).…”

The calcium-sensing receptor in bone metabolism: from bench to bedside and back

“…In MC3T3-E1 osteoblasts, the activation of CaSR resulted in an activation of K+-dependent channels, demonstrating that CaSR in osteoblasts is also functionally active [13]. Further studies using this cell system demonstrated that the chemotaxis of osteoblasts observed in the presence of elevated [Ca 2+ o ] was dependent upon phosphorylation of phospholipase C-γ1 (PLC-γ1) downstream of PLC-β in a protein kinase C (PKC)-dependent fashion [33]. The mitogenic action of CaSR and the consequent effect on osteoblast proliferation were paralleled by a concomitant activation of the Jun-terminal kinase (JNK) pathway and upregulation of several cell-cycle-associated genes (cyclin D1, D2, and D3 genes) and oncogenes in primary osteoblast cultures obtained from rat calvaria [34].…”