Calcium salicylate‐mediated apoptosis in human HT‐1080 fibrosarcoma cells

Mahdi, Jassem G.; Alkarrawi, M. A.; Mahdi, Ali Jassem; Bowen, I. D.; Humam, D.

doi:10.1111/j.1365-2184.2006.00390.x

Cited by 41 publications

(44 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The results suggest the correlations among the components present in MTA Fillapex formulation, like salicylate resin, diluting resin and silica with the cytotoxic effects. Salycilate resin has stimulated the process of apoptosis in human fibrosarcoma and has caused the fragmentation of cell genetic material, determining its precipitation in the cytoplasm (17). Arsenic, a heavy metal found as a possible contaminant in MTA (18) could be also related to MTA Fillapex cytotoxicity.…”

Section: Resultsmentioning

confidence: 99%

Long-Term Cytotoxicity, pH and Dissolution Rate of AH Plus and MTA Fillapex

et al. 2016

View full text Add to dashboard Cite

The aim of the present study was to verify the long-term cytotoxic effects of the MTA Fillapex and to compare them with AH Plus. Dissolution rate and pH were also evaluated. Human osteoblast cells were incubated with elutes of fresh specimens from AH Plus and MTA Fillapex, and with elutes of the same specimens for 4 successive weeks. Elute's pH was evaluated at each time point. A multiparametric cell viability assay was performed. For dissolution rate, ISO methodology was used. The results were analyzed by one-way analysis of variance, complemented with the Tukey post-test (p<0.05). No significant difference was found among the materials when fresh mixed (p>0.05). After 1 week, AH Plus became non-cytotoxic on all three evaluated parameters. Conversely, MTA Fillapex remained severely and mildly cytotoxic over the entire experimental period (p<0.05). The dissolution rate of AH Plus was significantly lower than MTA Fillapex at all time points (p>0.05). The pH of AH Plus was significantly lower than MTA Fillapex at the second and third week (p<0.05). In the other tested time points no statistical difference was observed.In conclusion, MTA Fillapex remained cytotoxic after 4 weeks and its cytotoxicity may be related to the high dissolution rate of this material.

show abstract

Section: Resultsmentioning

confidence: 99%

Long-Term Cytotoxicity, pH and Dissolution Rate of AH Plus and MTA Fillapex

et al. 2016

View full text Add to dashboard Cite

show abstract

“…For example, earlier work has highlighted the potential for 4-HBZn to induce apoptosis, particularly in primary CLL and other cancer cells [4,16] . HBCa compounds have also shown their apoptotic potential in human fibrosarcoma HT-1080 cells [10] . Metal ions including calcium (Ca ) showed improved efficacy to various organic compounds against cancer cells.…”

Section: Discussionmentioning

confidence: 99%

“…Metal ions including calcium (Ca ) showed improved efficacy to various organic compounds against cancer cells. For example, the incorporation of Zn 2+ or Ca 2+ ions improved the apoptotic potentials of HBA compounds [4,10,16] . In addition, platinum-based anticancer drugs have proven to exert highly effective therapeutic potentials in various cancer types [17] .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The effect of hydroxybenzoate calcium compounds in inducing cell death in epithelial breast cancer cells

Merghani¹,

Hazzaa²,

Mahdi³

et al. 2015

Adv Mod Oncol Res

View full text Add to dashboard Cite

Hydroxybenzoate (HB) compounds have shown their significance in inducing apoptosis in primary chronic lymphocytic leukemia (CLL) and cancer cell lines, including HT-1080. The current study focuses on assessing the effects of 2-, 3-and 4-hydroxybenzoate calcium (HBCa) compounds on MCF-10A, MDA-MB231 and MCF-7 epithelial breast cell lines. The HBCa-treated cells were examined using annexin V, to measure apoptosis in the three epithelial breast cell lines, after 48 h of treatment. The results indicated that 0.5 and 2.5 mmol/L of HBCa induced cell death in a dose-dependent manner. The induction of cell death in normal MCF-10A cells was found to be significantly less (p = 0.0003-0.0068), in comparison to the malignant cell lines (MDA-MB231 and MCF-7). HBCa compounds were also found to cause cell cycle arrest in the epithelial breast cells at G1/G0. Furthermore, HBCa compounds induced the upregulation of apoptotic proteins (p53, p21, Bax and caspase-3), as well as the downregulation of the anti-apoptotic protein Bcl-2, which may suggest that apoptosis is induced via the intrinsic pathway. reast cancer is a major global health issue that mainly affects women of all age groups. It is the most common cancer worldwide, with more cases in developing countries than in developed countries [1] . Breast cancer is the second leading cause of cancer death after lung cancer in developed countries (198,000 cases, 15.4%). Incidence rates continue to increase globally except in a few high-income countries. The estimated breast cancer incidents of less developed cases in 2012 were 883,000 (52.8%) and 788,000 more developed cancer cases, with mortality rates of 324,000 (62.1%) and 198,000 (37.9%), respectively [2] . In contrast, more than 60% of breast cancer patients survive in developed countries. Lower survival rates occur in developing countries due to the lack of early detection schemes and diagnosis [3] . Statistics related to breast cancer cases have attracted the attention of various researchers to effectively treat the cancer with chemotherapy. As part of this effort, a large number of compounds have been assessed for their anticancer potential in dif-B

show abstract

“…[20][21] ASA activates different signal pathways and causes apoptosis. 22 ASA used in hepatocellular carcinoma (HCC), prevents lung metastasis by decreasing the quantities of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). 23 There are various studies in respect of the usefulness of etoposide in HCC treatment.…”

mentioning

confidence: 99%

The Role of Acetylsalicylic Acid as an Assistant Drug in the Management of Hepatocellular Carcinoma

Alaçam¹

2012

UHOD

View full text Add to dashboard Cite

Glucose regulated protein 78 (Grp78) is a chaperon which acts in the protein folding. Cell-surface Grp78 is present on the surface of different type cancer cells. There are evidences that acetylsalicylic acid (ASA) provides advantages when combined with other chemotherapeutic agents in cancer patients. Resistance is an important problem in cancer treatment and etoposide is a drug which develops resistance against itself. Our purpose in this study is to see how the ASA, either used alone or combined with etoposide, affect on the HepG2 cell viability and to investigate the relationship of this viability with the cell-surface Grp78. Thus 12 different groups were assigned. Control group, 0.5 µg/ml etoposide group, 2.5, 5, 10 mM ASA groups, 10 mM 2-deoxyglucose (2-DG) + 0.5 µg/ml etoposide group and the groups added to this group with 2.5, 5 and 10 mM ASA respectively, 10 mM 2-DG +2.5 mM ASA group, 10 mM 2-DG + 5 mM ASA group, 10 mM 2-DG + 10 mM ASA group. MTT test was applied for the follow up of the viability of the cells. When the viability in etoposite group was %84.5, the viability in 2-DG+etoposit group was %75.4. This shows that 2-DG decreases the viability of the cells a little. Also, when ASA is applied alone or combined with 2-DG, it caused decrease in the cell viability but this effect was more significant when combined with 2-DG. When etoposide and ASA were combined, the decrease in high-dose viability was more significant. Cell surface Grp78 levels were higher in the group in which 2-DG and ASA were combined each other than the group in which ASA was applied alone. All these results show that cell-surface Grp78 is important for ASA showing its effect. New treatment protocols focused on the cell-surface Grp78 in cancer treatment can be revealed by further studies.Keywords: Grp78, Cell-surface Grp78, HepG2, Etoposide, Acetylsalicylic acid ÖZET Hepatosellüler Karsinoma Tedavisinde Yard›mc› bir ‹laç Olarak Asetilsalisilik Asit'in RolüGlucose regulated protein 78 (Grp78) protein katlanmas›nda rol oynayan bir flaperondur. Cell-surface Grp78 farkl› türde kanser hüc-relerinin yüzeyinde bulunmaktad›r. Acetylsalicylic acid (ASA)'n›n kanser hastalar›nda kullan›m›n›n fayda sa¤lad›¤›na dair kan›tlar vard›r. Kanser tedavisinde direnç önemli bir problemdir ve etoposide kendisine direnç geliflen bir ilaçt›r. Bizim bu çal›flmadaki amac›m›z etoposide ile kombine olarak ya da tek bafl›na kullan›lan ASA'n›n HepG2 hücre viabilitesi üzerine nas›l etki etti¤ini görmek ve bu canl›l›-¤›n cell-surface Grp78 ile iliflkisini araflt›rmakt›r. Bu amaçla 12 farkl› grup oluflturuldu. Kontrol grubu, 0.5 µg/ml etoposid grubu, 2.5, 5, 10 mM ASA gruplar›, 10 mM 2-deoxyglucose (2-DG) + 0.5 µg/ml etoposid içeren grup ve bu gruba s›ras›yla 2.5, 5 ve 10 mM ASA eklenen gruplar, 10 mM 2-DG +2.5 mM ASA grubu, 10 mM 2-DG + 5 mM ASA grubu, 10 mM 2-DG + 10 mM ASA grubu. Hücre-lerin canl›l›klar›n›n izlenmesi içinde MTT testi yap›ld›. Sadece etoposit verilen grupta canl›l›k %84.5 iken, 2-DG+etoposit verilen grupta canl›l›k %75.4 kadard›. 2-DG'n...

show abstract

Calcium salicylate‐mediated apoptosis in human HT‐1080 fibrosarcoma cells

Cited by 41 publications

References 50 publications

Long-Term Cytotoxicity, pH and Dissolution Rate of AH Plus and MTA Fillapex

Long-Term Cytotoxicity, pH and Dissolution Rate of AH Plus and MTA Fillapex

The effect of hydroxybenzoate calcium compounds in inducing cell death in epithelial breast cancer cells

The Role of Acetylsalicylic Acid as an Assistant Drug in the Management of Hepatocellular Carcinoma

Contact Info

Product

Resources

About