Calcium restores prepenetration morphogenesis abolished by polyamines in<i>Colletotrichum gloeosporioides</i>infecting red pepper

Ahn, Il-Pyung; Park, Jae Hyeon; Choi, Won San; Lee, Yong-Hwan

doi:10.1016/s0378-1097(03)00686-4

Cited by 18 publications

(14 citation statements)

References 21 publications

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“…Therefore, the degree of CmCaM expression in addition to its activation through Ca 2+ binding should also affect appressorium formation. Increased calmodulin transcript accumulation during cell development has been shown in M. grisea (Liu and Kolattukudy 1999), in C. gloeosporioides virulent on avocado and red pepper (Ahn et al 2003a;Kim et al 1998), and in C. trifolii (Warwar et al 2000).…”

Section: Discussionmentioning

confidence: 93%

“…Extensive studies using a wide range of pharmacological agents, which disrupt Ca 2+ infl uxes or interfere with the calcium-binding protein, have strongly implicated the involvement of Ca 2+ -signaling systems in appressorium formation by Colletotrichum species (Ahn et al 2003a, b;Uhm et al 2003) and the rice blast pathogen, Magnaporthe grisea (Lee and Lee 1998). However, the signaling pathways involved in infection-related morphogenesis in C. miyabeanus pathogenic on rice plant have not been characterized.…”

Section: Introductionmentioning

confidence: 99%

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Calcium/calmodulin-dependent signaling for prepenetration development in Cochliobolus miyabeanus infecting rice

Ahn

Suh

2007

J Gen Plant Pathol

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Cochliobolus miyabeanus forms a specialized infection structure, an appressorium, to infect rice. Contacting a hard surface induces appressorium formation in C. miyabeanus, while the hydrophobicity of the substratum does not affect this morphogenic infection event. To determine whether the calcium/calmodulin-dependent signaling system is involved in prepenetration morphogenesis in C. miyabeanus, the effects of a calcium chelator (ethylene glycol tetraacetic acid; EGTA), phospholipase C inhibitor (neomycin), intracellular calcium channel blocker (TMB-8), calmodulin antagonists (chlorpromazine, phenoxybenzamine, and W-7), and calcineurin inhibitor (cyclosporin A) on morphogenesis and infection were examined. Addition of Ca 2+ and the calcium ionophore A23187 did not affect conidial germination, while the number of appressoria decreased with higher concentrations. EGTA inhibited conidial germination and appressorium formation. The calcium channel blocker did not affect appressorium formation at any concentration; however, calmodulin antagonists and the calcineurin inhibitor specifi cally reduced appressorium formation at the micromolar level. One of the calmodulin antagonists, W-7, also inhibited accumulation of mRNA of the calmodulin gene within germinating conidia and/or appressorium-forming germ tubes. Thus, biochemical processes controlled by the calcium/calmodulin signaling system seem to be involved in the induction of prepenetration morphogenesis on rice.

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Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Calcium/calmodulin-dependent signaling for prepenetration development in Cochliobolus miyabeanus infecting rice

Ahn

Suh

2007

J Gen Plant Pathol

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“…These include a putative P-type Ca 2þ -ATPase, a calmodulin, a leucine zipper EF-hand protein, and a Ca 2þ -induced Ras inactivator (CAPRI). Several lines of evidence have demonstrated the involvement of calcium and calmodulin-dependent signaling during appressorium formation in several plant-interacting fungi (Ahn et al, 2003;Lee and Lee, 1998;Liu and Kolattukudy, 1999;Shaw and Hoch, 2000;Warwar et al, 2000). In M. grisea the use of Ca 2þ -inhibitors and chelators has shown to inhibit appressoria formation (Lee and Lee, 1998).…”

Section: Discussionmentioning

confidence: 99%

Recognition events in AM symbiosis: analysis of fungal gene expression at the early appressorium stage

Breuninger

Requena

2004

Fungal Genetics and Biology

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“…In addition, the Samdc inhibitor MGBG also affected both biological processes [47]. Interestingly, this effect was overcome by the addition of calcium, suggesting the involvement of the calmodulin/calcineurin signaling during the plant invasion process [47].…”

Section: Inhibition Of Polyamine Metabolismmentioning

confidence: 99%

Polyamine Metabolism in Fungi with Emphasis on Phytopathogenic Species

Valdés-Santiago

Cervantes-Chávez

León-Ramírez

et al. 2012

Journal of Amino Acids

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Polyamines are essential metabolites present in all living organisms, and this subject has attracted the attention of researchers worldwide interested in defining their mode of action in the variable cell functions in which they are involved, from growth to development and differentiation. Although the mechanism of polyamine synthesis is almost universal, different biological groups show interesting differences in this aspect that require to be further analyzed. For these studies, fungi represent interesting models because of their characteristics and facility of analysis. During the last decades fungi have contributed to the understanding of polyamine metabolism. The use of specific inhibitors and the isolation of mutants have allowed the manipulation of the pathway providing information on its regulation. During host-fungus interaction polyamine metabolism suffers striking changes in response to infection, which requires examination. Additionally the role of polyamine transporter is getting importance because of its role in polyamine regulation. In this paper we analyze the metabolism of polyamines in fungi, and the difference of this process with other biological groups. Of particular importance is the difference of polyamine biosynthesis between fungi and plants, which makes this process an attractive target for the control of phytopathogenic fungi.

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Calcium restores prepenetration morphogenesis abolished by polyamines inColletotrichum gloeosporioidesinfecting red pepper

Cited by 18 publications

References 21 publications

Calcium/calmodulin-dependent signaling for prepenetration development in Cochliobolus miyabeanus infecting rice

Calcium/calmodulin-dependent signaling for prepenetration development in Cochliobolus miyabeanus infecting rice

Recognition events in AM symbiosis: analysis of fungal gene expression at the early appressorium stage

Polyamine Metabolism in Fungi with Emphasis on Phytopathogenic Species

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