Calcium Receptor Binding of Cisplatin and Terbium in Human Breast Tumor Cells after Hyperthermia

Canada, Robert G.

doi:10.2307/3578353

Cited by 7 publications

(7 citation statements)

References 19 publications

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“…In addition, 90Y is easily separated by column chromatography from 90Sr, which has a very long half-life (28.8 years). It has been shown that Tm3+, Tb3+, and Yb3+ have a high affinity for tumor cells (24)(25)(26)(27). It is interesting to note that Tb3+ was temperature-dependently taken up by tumor cells (MCF-7), and cisplatin, a well-known anticancer drug, reduced the binding of Tb3+ to those tumor cells (25).…”

Section: Exposure To Rare Earthsmentioning

confidence: 99%

“…It has been shown that Tm3+, Tb3+, and Yb3+ have a high affinity for tumor cells (24)(25)(26)(27). It is interesting to note that Tb3+ was temperature-dependently taken up by tumor cells (MCF-7), and cisplatin, a well-known anticancer drug, reduced the binding of Tb3+ to those tumor cells (25). However, there is a contradictory report that has shown that La concentration in malignant laryngeal tissue was lower than in nonmalignant adjacent tissues, although serum La concentrations of laryngeal cancer patients were significantly higher than those of normal subjects (28).…”

Section: Exposure To Rare Earthsmentioning

confidence: 99%

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Exposure, metabolism, and toxicity of rare earths and related compounds.

Hirano

Suzuki

1996

Environ Health Perspect

344

120

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For the past three decades, most attention in heavy metal toxicology has been paid to cadmium, mercury, lead, chromium, nickel, vanadium, and tin because these metals widely polluted the environment. However, with the development of new materials in the last decade, the need for toxicological studies on those new materials has been increasing. A group of rare earths (RE) is a good example. Although some RE have been used for superconductors, plastic magnets, and ceramics, few toxicological data are available compared to other heavy metals described above. Because chemical properties of RE are very similar, it is plausible that their binding affinities to biomolecules, metabolism, and toxicity in the living system are also very similar. In this report, we present an overview of the metabolism and health hazards of RE and related compounds, including our recent studies.ImagesFigure 1. AFigure 1. BFigure 1. C

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Section: Exposure To Rare Earthsmentioning

confidence: 99%

Section: Exposure To Rare Earthsmentioning

confidence: 99%

Exposure, metabolism, and toxicity of rare earths and related compounds.

Hirano

Suzuki

1996

Environ Health Perspect

344

120

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“…The Yb 3+ -transferrin complex inhibited the uptake of the fluorescein labeled ferric-saturated transferrin (Fe 2 -transferrin) complex into K562 cells. The results demonstrate that the complex of Yb 3+ -transferrin complex was recognized by the transferrin receptor and that the transferrin-receptor-mediated mechanism is a possible pathway for Yb 3+ accumulation in cells.Keywords: K562 cells; recognition; transferrin; ytterbium.Lanthanides have been suggested for the treatment of a series of diseases and for diagnosis by magnetic resonance imaging [1,2]. Recent studies also show that they could act as scavengers of free radicals [3] and therefore protect cells and tissues from oxidative stress-induced injury.…”

mentioning

confidence: 99%

“…Lanthanides have been suggested for the treatment of a series of diseases and for diagnosis by magnetic resonance imaging [1,2]. Recent studies also show that they could act as scavengers of free radicals [3] and therefore protect cells and tissues from oxidative stress-induced injury.…”

mentioning

confidence: 99%

Complexation of ytterbium to human transferrin and its uptake by K562 cells

Zhang

Liu

et al. 2002

European Journal of Biochemistry

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There is an increasing interest in the use of lanthanides in medicine. However, the mechanism of their accumulation in cells is not well understood. Lanthanide cations are similar to ferric ions with regard to transferrin binding, suggesting transferrin-receptor mediated transport is possible; however, this has not yet been confirmed. In order to clarify this mechanism, we investigated the binding of Yb 3+ to apotransferrin by UV-Vis spectroscopy and stopped-flow spectrophotometry, and found that Yb 3+ binds to apotransferrin at the specific iron sites in the presence of bicarbonate. The apparent binding constants of these sites showed that the affinity of Yb 3+ is lower than that of Fe 3+ and binding of Yb 3+ in the N-lobe is kinetically favored while the C-lobe is thermodynamically favored. The first Yb 3+ bound to the C-lobe quantitatively with a Yb/ apotransferrin molar ratio of < 1, whereas the binding to the other site is weaker and approaches completeness by a higher molar ratio only. As demonstrated by 1 H NMR spectra, Yb 3+ binding disturbed the conformation of apotransferrin in a manner similar to Fe 3+ . Flow cytometric studies on the uptake of fluorescein isothiocyanate labeled Yb 3+ -bound transferrin species by K562 cells showed that they bind to the cell receptors. Laser scanning confocal microscopic studies with fluorescein isothiocyanate labeled Yb 3+ -bound transferrin and propidium iodide labeled DNA and RNA in cells indicated that the Yb 3+ entered the cells. The Yb 3+ -transferrin complex inhibited the uptake of the fluorescein labeled ferric-saturated transferrin (Fe 2 -transferrin) complex into K562 cells. The results demonstrate that the complex of Yb 3+ -transferrin complex was recognized by the transferrin receptor and that the transferrin-receptor-mediated mechanism is a possible pathway for Yb 3+ accumulation in cells.Keywords: K562 cells; recognition; transferrin; ytterbium.Lanthanides have been suggested for the treatment of a series of diseases and for diagnosis by magnetic resonance imaging [1,2]. Recent studies also show that they could act as scavengers of free radicals [3] and therefore protect cells and tissues from oxidative stress-induced injury. Some lanthanides nuclides were also suggested for palliative therapy. 169 Yb (c-emission, t ½ % 32 days) was reported to provide comparable tumor control and has been considered as a potential replacement for 125 I and 103 Pd in permanent implants [4,5]. Evidently, the intracellular accumulation is very important in these cases, but its mechanism still remains unclear.It was suggested that the particulate-and protein-bound Ln enters the cells by endocytosis [6]; the anionic lowmolecular-mass complexes, via anion channels [7], whereas free Ln 3+ is transported by ionophores [8], Na + /Ca 2+ exchange [9] and self-facilitated diffusion [10]. It is known that Ln 3+ is mainly bound to proteins in the extracellular media (e.g. plasma). Various studies have demonstrated that considerable amounts of Ln are bound to the iron transport prote...

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“…Além das diversas aplicações descritas anteriormente, terras raras há muito tempo têm sido investigados para o tratamento de doenças e para diagnóstico por imagem na técnica de ressonância magnética 6,7 . Estudos mais recentes mostram que poderiam agir como capturadores de radicais livres e, portanto proteger células e tecidos de dano induzido por fadiga oxidativa, com potenciais ações farmacológicas 8 .…”

Section: Segundo O Centro De Gestão E Estudos Estratégicosunclassified

Desenvolvimento de sistemas híbridos luminescentes - Polímero:európio(III) com estabilidade térmica e fotoluminescente

Forster¹

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Ao meu filho, Murilo Ribeiro Forster, por me dar a força mesmo quando ela não existe.Aos meus Pais, Vilma e Rubens, e minha irmã, Carla, pela educação base para minha vida e apoio nos meus estudos, e por tudo, sem vocês não sou ninguém.A minha orientadora, Prof a . Dr a . Duclerc Fernandes Parra, pelo tempo, confiança, por todos esses anos de IPEN, IC, Mestrado e Doutorado, por seus pelos "puxões" de orelha quando necessário e, sobretudo, por sua dedicação e infinita paciência comigo, por compartilhar seus conhecimentos comigo, pelo interesse e sugestões valiosas para o desenvolvimento desta Tese, Obrigado!

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Calcium Receptor Binding of Cisplatin and Terbium in Human Breast Tumor Cells after Hyperthermia

Cited by 7 publications

References 19 publications

Exposure, metabolism, and toxicity of rare earths and related compounds.

Exposure, metabolism, and toxicity of rare earths and related compounds.

Complexation of ytterbium to human transferrin and its uptake by K562 cells

Desenvolvimento de sistemas híbridos luminescentes - Polímero:európio(III) com estabilidade térmica e fotoluminescente

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