Calcium Protects Differentiating Neuroblastoma Cells during 50Hz Electromagnetic Radiation

Tonini, Raffaella; Baroni, Maurizio D.; Masala, Erico; Micheletti, Martina; Ferroni, A; Mazzanti, Michele

doi:10.1016/s0006-3495(01)75902-4

Cited by 40 publications

(20 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been reported that calcium ion content, calcium ion efflux, and insulin secretion during glucose stimulation was reduced when isolated rabbit islets were exposed to low frequency pulsed magnetic fields [Joelly et al, 1983]. It has also been reported that exposure to ELFMF affected neurite growth via voltage gated calcium channels [Morgado-Valle et al, 1998] and affected the differentiation of neuroblastoma cells by antagonizing the shift in cell membrane surface charges and increasing intracellular calcium levels [Tonini et al, 2001]. Our present findings are in agreement with these previous reports.…”

Section: Discussionsupporting

confidence: 92%

An extremely low frequency magnetic field attenuates insulin secretion from the insulinoma cell line, RIN‐m

Sakurai

Satake

Sumi

et al. 2004

Bioelectromagnetics

View full text Add to dashboard Cite

In this study, we investigated the effects of exposure to an extremely low frequency magnetic field (ELFMF) on hormone secretion from an islet derived insulinoma cell line, RIN-m. We stimulated RIN-m cells to secrete insulin under exposure to an ELFMF, using our established system for the exposure of cultured cells to an ELFMF at 5 mT and 60 Hz, or under sham exposure conditions for 1 h and observed the effects. In the presence of a depolarizing concentration of potassium (45 mM KCl), exposure to ELFMF significantly attenuated insulin release from RIN-m cells, compared to sham exposed cells. Treatment with nifedipine reduced the difference in insulin secretion between cells exposed to an ELFMF and sham exposed cells. The expression of mRNA encoding synaptosomal associated protein of 25 kDa (SNAP-25) and synaptotagmin 1, which play a role in exocytosis in hormone secretion and influx of calcium ions, decreased with exposure to an ELFMF in the presence of 45 mM KCl. These results suggest that exposure to ELFMF attenuates insulin secretion from RIN-m cells by affecting calcium influx through calcium channels.

show abstract

Section: Discussionsupporting

confidence: 92%

An extremely low frequency magnetic field attenuates insulin secretion from the insulinoma cell line, RIN‐m

Sakurai

Satake

Sumi

et al. 2004

Bioelectromagnetics

View full text Add to dashboard Cite

show abstract

“…Therefore, a potential involvement of different signal transduction pathways, including Ca 2+ signaling, in the proliferative response induced by the MF cannot be ruled out. In fact, calcium signaling has been described as a protecting factor against MF-induced damage in neuroblastoma cells [48]. The importance of Ca 2+ in the cellular response to ELF fields has been i nvestigated and reviewed by a number of authors [49][50][51][52][53].…”

Section: Discusionmentioning

confidence: 99%

The Proliferative Response of NB69 Human Neuroblastoma Cells to a 50 Hz Magnetic Field is mediated by ERK1/2 Signaling

Martínez

Úbeda

Cid³

et al. 2012

Cell Physiol Biochem

View full text Add to dashboard Cite

A number of studies have reported that extremely low frequency magnetic fields (ELF-MF) can modulate proliferative processes in vitro; however, the transduction mechanisms implicated in such phenomena remain to be identified. The present study was aimed to determine whether a 50 Hz, 100 µT MF can induce cell proliferation in the human neuroblastoma line NB69, and whether the signaling pathway MAPK-ERK1/2 (Mitogen-Activated Protein Kinase - Extracellular-Signal-Regulated Kinase 1 and 2) is involved in that proliferative response. The cultures were exposed intermittently or continuously to the MF for a 63-hour duration. The continuous treatment did not induce significant changes in cell proliferation. In contrast, intermittent exposure caused statistically significant increase in the percent of cells in phase S of the cell cycle, followed by a significant increase in cell number. The intermittent treatment also induced an early, transient and repetitive activation of ERK1/2 that could be involved in the proliferative effects. In fact, both the proliferative response and the repeated activation of ERK1/2 were blocked by PD98059, the specific inhibitor of MEK (ERK kinases 1 and 2). Taken together, the described results indicate that a 50 Hz, 100 µT MF can stimulate proliferation in NB69 cells by triggering MAPK-ERK1/ 2 signaling at each of the “On” periods of an intermittent exposure.

show abstract

“…Results of experimental investigations that demonstrated the EMF effect on enzymes (Ding et al, 2001) and alterations in the structure and function of cellular membranes (Lisi et al, 2000;Bordiushkov et al, 2000) which are appointed as primary targets of EMF action on biological systems (Tenforde;Kaune, 1987;Goodman;Greenebaum, Marron, 1995), implicate the possible aspect of a direct EMF influence on the thyroid gland (MATAVULJ et al, 1998) Apud Rajkovic et al (2003. Furthermore Tonini et al, (2001) in their study demonstrated that a 50/60-Hz magnetic field interacts with cell differentiation through two opposing mechanisms. ELF-EMF is able to prevent the shift in surface charges potential promoted by differentiating agents.…”

Section: Discussionmentioning

confidence: 99%

Effects of the Electromagnetic field, 60 Hz, 3 µT, on the hormonal and metabolic regulation of undernourished pregnant rats

2009

View full text Add to dashboard Cite

Epidemiological studies have implicated maternal protein-calorie deficiency as an important public health problem in developing countries. Over the last decades, a remarkable diffusion of electricity and an increased level of the electromagnetic field (EMF) in the environment have characterized modern societies. Therefore, researchers are concerned with the biological effects of 50-60 Hz, EMF. The aim of this paper is to show the effects of EMF of 60 Hz, 3 µT, exposure for two hours per day in the regulation of the hormonal and metabolic concentrations in pregnant rats, which were fed by Regional Basic Diet (RBD) during their pregnancy as compared with pregnant rats fed a standard diet. Pregnant rats exposed to EMF of 60 Hz, 3 µT, over the pregnancy and fed with RBD presented an increase in glucose release when compared with the Group subjected only to the RBD ration. Rats fed RBD presented a decrease in their insulin and cortisol serum levels when compared with the Group fed with casein. The T 3 and T 4 concentrations presented the greatest variation among the Groups. The relation T 4 :T 3 was much exaggerated in the Group subjected to RDB and exposed to EMF when compared to the others. In conclusion, the group subjected to the association of EMF and undernutrition suffered a decrease in its serum concentration of T 4 and T 3 when compared to the well-nourished group and the relationship T 4 :T 3 in the former group was almost eighteen-fold the later one.Keywords: casein, electromagnetic field, metabolism, hormone, regional basic diet, undernourished pregnant rats. Efeitos do campo eletromagnético, 60 Hz, 3µT, na regulação hormonal e metabólica de ratas prenhes desnutridas ResumoEstudos epidemiológicos têm mostrado que a deficiência proteico-calórica é um importante problema nos países em desenvolvimento. Durante as últimas décadas, a sociedade moderna tem sido caracterizada pelo aumento no número de equipamentos elétricos e como consequência um aumento no nível do campo eletromagnético (CEM) no ambiente. No entanto, os pesquisadores estão preocupados com os efeitos biológicos dos CEM de 50-60 Hz. O objetivo deste artigo é mostrar os efeitos do CEM de 60 Hz, 3 µT, nas concentrações hormonais e metabólicas de ratas prenhes, expostas duas horas por dia ao CEM, alimentadas pela Dieta Básica Regional (DBR) comparando com ratas submetidas às mesmas condições, mas alimentas com dieta padrão. Ratas prenhes expostas ao CEM de 60 Hz, 3 µT, durante a prenhez e alimentadas com a DBR apresentaram um aumento na liberação de glicose quando comparadas com o grupo alimentado pela DBR sem CEM. As ratas alimentadas pela DBR apresentaram uma diminuição nos níveis de insulina e cortisol quando comparadas com o grupo alimentado pela caseína. As concentrações de T 3 e T 4 apresentaram a maior variação entre os grupos. A relação T 4 :T 3 foi muito exagerada no grupo alimentado pela DBR e exposto ao CEM quando comparado com os outros. Conclusão, os animais que foram submetidos à desnutrição e ao CEM sofreram uma diminuição na ...

show abstract

Calcium Protects Differentiating Neuroblastoma Cells during 50Hz Electromagnetic Radiation

Cited by 40 publications

References 35 publications

An extremely low frequency magnetic field attenuates insulin secretion from the insulinoma cell line, RIN‐m

An extremely low frequency magnetic field attenuates insulin secretion from the insulinoma cell line, RIN‐m

The Proliferative Response of NB69 Human Neuroblastoma Cells to a 50 Hz Magnetic Field is mediated by ERK1/2 Signaling

Effects of the Electromagnetic field, 60 Hz, 3 µT, on the hormonal and metabolic regulation of undernourished pregnant rats

Contact Info

Product

Resources

About