Calcium, Phosphorus and Oxygen Around Implant at Early Osseointegration in Hyperlipidemic Rats

Abstract

The study was to observe dental implant osseointegration at early stage and analysis the influencement of the ratio of calcium and phosphorus variation on osseointegration in hyperlipidemic rats. Wistar rats were randomly divided into control group with normal diet and experimental group with high fat diet. 8 weeks later, serum lipid levels was detected and titanium implants were placed into bilateral femurs. At day1, 3, 5 after implant, rats were sacrificed and 5 mm bone with implants was obtained. hard tissue slices and methylene blue-acid fuchsin staining were performed to observe the interface of bone-implant. And energy disperse X-ray spectrometer (EDS) was used to measure the percentage of oxygen and the ratio of calcium and phosphorus. All rats in experimental group were successfully induced into hyperlipidemic status. Histological results revealed less osteoblasts and trabecular bone, but more osteoclasts and trabecular space in experimental group than those in control group. Higher content of oxygen and lower ration of calcium and phosphorus were also observed in experimental group than those in control group (P<0.05). Hyperlipidemia could hinder implant osseointegration at early stage after implantation. This inhibition might be closely related to higher content of oxygen and lower ratio of calcium and phosphorus.