Calcium phosphate mineralization through homogenous enzymatic catalysis: Investigation of the early stages

Colaço, Elodie; Brouri, Dalil; Méthivier, Christophe; Valentin, Laetitia; Oudet, F.; Kirat, Karim El; Guibert, Clément de; Landoulsi, Jessem

doi:10.1016/j.jcis.2019.12.097

Cited by 32 publications

(36 citation statements)

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“…Thus, control mechanisms such as enzymatic kinetics need to also be involved. Our first results in homogeneous phase, from [ 124 ], already showed that, while ACP forms initially as in classic in vitro experiments, the crystallization pathway is different compared to abiotic procedures when no enzyme is involved.…”

Section: Prospects/future Challengesmentioning

confidence: 99%

“…In an earlier study [ 124 ], we demonstrated that CaP mineralization could be initiated through a homogeneous enzymatic catalysis. Calcium (II) ions and an orthophosphate precursor, namely α-glycerol phosphate magnesium salt, were mixed together with the alkaline phosphatase (ALP) enzyme.…”

Section: Enzymatic Approachmentioning

confidence: 99%

“…This induction time reflects the ability of the solution to maintain a metastable equilibrium state [ 125 , 126 ]. On the contrary, when the Pi is generated through the enzymatic consumption of a substrate S, such as glycerol phosphate [ 124 ], σ is initially not defined ( Ez , Figure 4 A). When the substrate is added, σ starts to increase, following the enzymatic kinetic release of Pi until the critical value required to start the mineralization is achieved (blue arrow pointing up).…”

Section: Enzymatic Approachmentioning

confidence: 99%

“…Thus, in [ 124 ], various mineralization conditions were explored, and several original results concerning the effect of the enzymatic process were analyzed:…”

Section: Enzymatic Approachmentioning

confidence: 99%

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Enzymatic Approach in Calcium Phosphate Biomineralization: A Contribution to Reconcile the Physicochemical with the Physiological View

Guibert

Landoulsi

2021

IJMS

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Biomineralization is the process by which organisms produce hard inorganic matter from soft tissues with outstanding control of mineral deposition in time and space. For this purpose, organisms deploy a sophisticated “toolkit” that has resulted in significant evolutionary innovations, for which calcium phosphate (CaP) is the biomineral selected for the skeleton of vertebrates. While CaP mineral formation in aqueous media can be investigated by studying thermodynamics and kinetics of phase transitions in supersaturated solutions, biogenic mineralization requires coping with the inherent complexity of biological systems. This mainly includes compartmentalization and homeostatic processes used by organisms to regulate key physiological factors, including temperature, pH and ion concentration. A detailed analysis of the literature shows the emergence of two main views describing the mechanism of CaP biomineralization. The first one, more dedicated to the study of in vivo systems and supported by researchers in physiology, often involves matrix vesicles (MVs). The second one, more investigated by the physicochemistry community, involves collagen intrafibrillar mineralization particularly through in vitro acellular models. Herein, we show that there is an obvious need in the biological systems to control both where and when the mineral forms through an in-depth survey of the mechanism of CaP mineralization. This necessity could gather both communities of physiologists and physicochemists under a common interest for an enzymatic approach to better describe CaP biomineralization. Both homogeneous and heterogeneous enzymatic catalyses are conceivable for these systems, and a few preliminary promising results on CaP mineralization for both types of enzymatic catalysis are reported in this work. Through them, we aim to describe the relevance of our point of view and the likely findings that could be obtained when adding an enzymatic approach to the already rich and creative research field dealing with CaP mineralization. This complementary approach could lead to a better understanding of the biomineralization mechanism and inspire the biomimetic design of new materials.

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Section: Prospects/future Challengesmentioning

confidence: 99%

Section: Enzymatic Approachmentioning

confidence: 99%

Section: Enzymatic Approachmentioning

confidence: 99%

“…Thus, in [ 124 ], various mineralization conditions were explored, and several original results concerning the effect of the enzymatic process were analyzed:…”

Section: Enzymatic Approachmentioning

confidence: 99%

See 2 more Smart Citations

Enzymatic Approach in Calcium Phosphate Biomineralization: A Contribution to Reconcile the Physicochemical with the Physiological View

Guibert

Landoulsi

2021

IJMS

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“…In addition, the concomitant carbonate tends to be massively incorporated in solids, as in the case of layered double hydroxides (LDHs), [24] giving birth to non-stoichiometric phases bearing variable calcium to phosphate ratios. [25] Enzymatically-driven growth at room temperature, employing phosphatases [26,27] or ureases, [28][29][30] has been employed as an alternative to overcome this limitation. However, these approaches are expensive and hardly reproducible, limiting the possibilities of scaled up synthesis.…”

Section: Introductionmentioning

confidence: 99%

Amorphous Calcium Phosphates: Solvent‐Controlled Growth and Stabilization through the Epoxide Route

Borovik

Oestreicher

Huck‐Iriart

et al. 2021

Chemistry A European J

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Calcium phosphates stand among the most promising nanobiomaterials in key biomedical applications, such as bone repairment, signalling or drug/gene delivery. Their intrinsic properties as crystalline structure, composition, particle shape and size define their successful use. Among these compounds, metastable amorphous calcium phosphate (ACP) is currently gaining particular attention due to its inherently high reactivity in solution, which is crucial in bone development mechanisms. However, the preparation of this highly desired (bio)material with control over its shape, size and phase purity remains as a synthetic challenge. In this work, the epoxide route was adapted for the synthesis of pure and stable ACP colloids. By using biocompatible solvents, such as ethylene glycol and/or glycerine, it was possible to avoid the natural tendency of ACP to maturate into more stable and crystalline apatites. Moreover, this procedure offers size control, ranging from small nanoparticles (60 nm) to micrometric spheroids (>500 nm). The eventual fractalization of the internal mesostructured can be tuned, by simply adjusting the composition of the ethylene glycol:glycerine solvent mixture. These findings introduce the use of green solvents as a new tool to control crystallinity and/or particle size in the synthesis of nanomaterials, avoiding the use of capping agents and preserving the natural chemical reactivity of the pristine surface.

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Self‐Activating Phosphorus‐Rich Substrate Import Enzyme Catalytic Domains for Bone Regeneration

Zhao,

Chen,

Tang

et al. 2024

Adv Funct Materials

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Enzymes play a key role in natural mineralization, but their function is disabling in bone metabolism when patients suffer severe degenerations like hypophosphatasia. To regulate bone homeostasis, a self‐activating phosphorus‐rich system is proposed on calcium glycerophosphate (CaGP) modified polyurethane (PU) fibrous substrate, wherein ample enzyme catalytic domains are imported with intestinal alkaline phosphatase (IALP) chelating and activating by calcium in situ that is testified by chemical potential evaluation. Therefore, Ca‐bonding IALP catalyzes organophosphate to phosphate locally to achieve a calcified symbiotic state, regulating mineralization and accelerating osteoinduction in vitro and in vivo. The self‐activating enzyme system promotes early osteogenic differentiation and angiogenesis, resulting in rapid healing of cranial defect with a “long‐tail” effect compared to an enzyme‐assisted system. Transcriptomic and metabolomic analyses demonstrate positive associations with neovascularization and calcium/phosphorus metabolism, specifically upregulated glycerophospholipids and calcium ions in favor of osteogenic differentiation under cytokine‐free conditions. These results provide a potential enzyme‐activating therapy in bone homeostasis and regeneration.

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Calcium phosphate mineralization through homogenous enzymatic catalysis: Investigation of the early stages

Cited by 32 publications

References 50 publications

Enzymatic Approach in Calcium Phosphate Biomineralization: A Contribution to Reconcile the Physicochemical with the Physiological View

Enzymatic Approach in Calcium Phosphate Biomineralization: A Contribution to Reconcile the Physicochemical with the Physiological View

Amorphous Calcium Phosphates: Solvent‐Controlled Growth and Stabilization through the Epoxide Route

Self‐Activating Phosphorus‐Rich Substrate Import Enzyme Catalytic Domains for Bone Regeneration

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