Calcium Phosphate Ceramics as Substrate for Cartilage Cultivation

Janßen, Rolf; Nagel-Heyer, Stephanie; Goepfert, Christine; Pörtner, Ralf; Toykan, D.; Krummhauer, O.; Morlock, Michael M.; Adamietz, Peter; Meenen, N. M.; Kriven, Waltraud M.; Kim, Dong Kyu; Tampieri, Anna; Celotti, G.

doi:10.1002/9780470291191.ch79

Cited by 7 publications

(7 citation statements)

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“…The solid carrier was made of cured bone cement (Calcibon, diameter 4.55 mm, height 2 mm, donated by Biomet Deutschland, Darmstadt, Germany) which consisted of carbonated calcium deficient hydroxyapatite [13]. Calcibon was shown to be an osteoconductive material in trabecular bone [19].…”

Section: Cells and Culture Conditionsmentioning

confidence: 99%

Bioreactor cultivation of three-dimensional cartilage-carrier-constructs

Nagel-Heyer

Goepfert

Feyerabend

et al. 2005

Bioprocess Biosyst Eng

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A flow-chamber bioreactor was designed for generation of three-dimensional cartilage-carrier-constructs. A specific attribute of the flow-chamber is a very thin medium layer for improved oxygen supply and a counter current flow of medium and gas. Three-dimensional cartilage-carrier-constructs were produced according to a standard protocol from chondrocytes of an adult mini-pig. The final step of this protocol was performed either in the bioreactor or in 12-well plates. The bioreactor experiments showed a significantly higher matrix thickness but a lower ratio of glycosaminoglycan to DNA. For both culture methods the constructs contained a high amount of collagen II. Appearance of the cartilage obtained in the bioreactor seemed to be closer to native cartilage with respect to distribution of the cells within the matrix, smoothness of the surface etc. All results considered the flow-chamber bioreactor is a very useful tool for generation of three dimensional cartilage-carrier constructs.

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Section: Cells and Culture Conditionsmentioning

confidence: 99%

Bioreactor cultivation of three-dimensional cartilage-carrier-constructs

Nagel-Heyer

Goepfert

Feyerabend

et al. 2005

Bioprocess Biosyst Eng

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“…6. As can be seen, the as‐attrition‐milled powders still retained their amorphous state as is usual for many other kinds of powders prepared by the same technique 34–42 . For the sample “sintered” at 900°C, the original, wide, broad peak between 25° and 35° in 2θ disappeared.…”

Section: Resultsmentioning

confidence: 63%

“…The material fabrication technique includes powder synthesis by using the organic steric entrapment (PVA) method invented in our laboratory and used for preparation of many kinds of oxide powders, 36–44 followed by a powder attrition milling treatment, cold isostatic pressing, and pressureless sintering. The processing flowchart for synthesizing the porous gehlenite ceramic is shown in Fig.…”

Section: Methodsmentioning

confidence: 99%

Sintering Behavior of Gehlenite. Part I: Self‐Forming, Macro‐/Mesoporous Gehlenite—Pore‐Forming Mechanism, Microstructure, Mechanical, and Physical Properties

Jia

Kim

Kriven

2007

Journal of the American Ceramic Society

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A novel kind of pore self-forming macro-/mesoporous gehlenite (2CaO . Al 2 O 3 . SiO 2 ) ceramic (abbreviated C 2 AS) having a highest porosity of 80% corresponding to a volume expansion of 134% during sintering has been developed. The pore selfforming ability, microstructure, mechanical, and thermal physical properties of the porous ceramic are related to the sintering temperature. The gehlenite ceramic shows a very good pore selfforming ability over a very wide range of temperature from 9001 to 14501C. No vesicant is required and no hydrothermal treatment is needed, as is generally the case for other kinds of porous ceramics or glasses. The pore self-forming ability of the C 2 AS porous ceramic can be attributed to the escape of the adsorbed water vapor during the sintering process, due to automatic hydration of the fine, amorphous, flakey-shaped starting C 2 AS powder particles synthesized by the organic steric entrapment (PVA) method, as well as to their fine, porous microstructure. The pores of the ceramics can be either open or closed, and the average pore size ranges from 0.6 to 1.1 lm, corresponding to a porosity of 75%-80%, respectively. The porous ceramic can preserve nanometer-sized (26-50 nm) crystallites up to 10001C. Sintered or thermally treated under different conditions, the porous ceramics exhibit relatively high flexural strengths ranging from 9.1 to 15.4 MPa, with a standard deviation of 0.3 and 4.2 MPa, respectively. Thermal properties of the porous ceramic up to 10001C, including thermal expansion coefficient, thermal diffusivity, specific heat, and thermal conductivity, were investigated, and the stability of the porous ceramic in boiling water was also studied.

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“…T he organic steric entrapment (PVA and PEG) method was invented in our laboratory 1 and used to fabricate a variety of one‐, two‐, and three‐component oxides including alumina, cristabolite, mullite, YAG, xenotime, calcium mono‐ and tri‐aluminates, as well as mono‐, di‐, and tri‐calcium silicates, cordierite, leucite, zircon, the components of Portland cement, and various other titanates and phosphates 2–15 . The technique is highly recommended because it has higher powder yield than do other chemical synthesis methods and it can even be extended to chemicals that decompose in water 2 .…”

Section: Introductionmentioning

confidence: 99%

“…T HE organic steric entrapment (PVA and PEG) method was invented in our laboratory 1 and used to fabricate a variety of one-, two-, and three-component oxides including alumina, cristabolite, mullite, YAG, xenotime, calcium mono-and tri-aluminates, as well as mono-, di-, and tri-calcium silicates, cordierite, leucite, zircon, the components of Portland cement, and various other titanates and phosphates. [2][3][4][5][6][7][8][9][10][11][12][13][14][15] The technique is highly recommended because it has higher powder yield than do other chemical synthesis methods and it can even be extended to chemicals that decompose in water. 2 In a recent paper by us, the PVA method was used to prepare macro-/mesoporous gehlenite (2CaO Á Al 2 O 3 Á SiO 2 or C 2 AS) ceramic with a very good pore self-forming ability, but our original target was to prepare a dense gehlenite ceramic to find a ceramic matrix candidate more compatible with dicalcium silicate, which could exhibit a phase transformation toughening effect.…”

Section: Introductionmentioning

confidence: 99%

Sintering Behavior of Gehlenite, Part II. Microstructure and Mechanical Properties

Jia

Kriven

2007

Journal of the American Ceramic Society

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A self‐toughened gehlenite (2CaO·Al2O3·SiO2 or “C2AS”) ceramic with randomly distributed platelet grains was prepared by the organic steric entrapment (PVA) route. The gehlenite ceramic had a density of 2.698–2.875 g/cm3, corresponding to a relative density of 90%–96%. The platelet gehlenite grains had an average thickness of 3.6±0.8 μm and a width of 12.9±3.7 μm, respectively, with an average aspect ratio of 3.6. The three‐point bending strength, fracture toughness, and Young's modulus attained were 142.1±12.1 MPa, 2.32±0.12 MPa·m1/2, and 108±6.8 GPa, respectively. Fractography as well as Vickers indentation crack propagation profiles showed that crack deflection, crack blunting, and pinning effects due to the randomly distributed platelet grains were considered to be responsible for the good mechanical properties of the gehlenite ceramic.

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Calcium Phosphate Ceramics as Substrate for Cartilage Cultivation

Cited by 7 publications

References 0 publications

Bioreactor cultivation of three-dimensional cartilage-carrier-constructs

Bioreactor cultivation of three-dimensional cartilage-carrier-constructs

Sintering Behavior of Gehlenite. Part I: Self‐Forming, Macro‐/Mesoporous Gehlenite—Pore‐Forming Mechanism, Microstructure, Mechanical, and Physical Properties

Sintering Behavior of Gehlenite, Part II. Microstructure and Mechanical Properties

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