2003
DOI: 10.1074/jbc.m211271200
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Calcium Modulates Promoter Occupancy by the Entamoeba histolytica Ca2+-binding Transcription Factor URE3-BP

Abstract: The Entamoeba histolytica upstream regulatory element 3-binding protein (URE3-BP) binds to the URE3 sequence of the Gal/GalNAc-inhibitable lectin hgl5 and ferredoxin 1 (fdx) gene promoters. This binding can be inhibited in vitro by addition of calcium. Two EF-hand motifs, which are associated with the ability to bind calcium, are present in the amino acid sequence of URE3-BP. Mutation of the second EF-hand motif in URE3-BP resulted in the loss of calcium inhibition of DNA binding as monitored by electrophoreti… Show more

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Cited by 31 publications
(27 citation statements)
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“…Intracellular Ca 2ϩ fluxes have been observed in E. histolytica upon contact with fibronectin (6) and increased extracellular Ca 2ϩ concentrations (18). The precise Ca 2ϩ concentration of fluxes induced by these extracellular cues has not been determined.…”
Section: Discussionmentioning
confidence: 99%
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“…Intracellular Ca 2ϩ fluxes have been observed in E. histolytica upon contact with fibronectin (6) and increased extracellular Ca 2ϩ concentrations (18). The precise Ca 2ϩ concentration of fluxes induced by these extracellular cues has not been determined.…”
Section: Discussionmentioning
confidence: 99%
“…URE3-BP localized to both the nucleus and the cytoplasm of trophozoites and was also found to be enriched in the plasma membrane. Ca 2ϩ induced the release of binding of URE3-BP from the URE3 motif in vitro and in vivo (18). Mutation of the second EF-hand motif at residues critical for Ca 2ϩ coordination abrogated this effect and allowed for the genome-wide identification of targets of URE3-BP in parasites overexpressing the mutant protein (16).…”
mentioning
confidence: 99%
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“…The differences were noticed beyond 670 nucleotides upstream of the translation initiation codon ATG. Sequence conservation of the upstream 670 nucleotides was surprising as most of the amoebic promoters have been found very close to the gene (11). It is likely that either the specific promoters or the controlling elements are beyond 670 nucleotides or that an epigenetic mechanism may be involved in determining the expression status of these genes (6).…”
Section: Discussionmentioning
confidence: 99%
“…Signal transduction pathways involving calcium have also been identified (27,28). These pathways are mediated through a number of calcium binding proteins, including calmodulin (24), a novel calcium binding transcription regulator (11), and a novel protein involved in actin cytoskeletal dynamics (30).…”
mentioning
confidence: 99%