Calcium-Modulated Proteins Change Their Immunoreactivity in the Presence of Ca2+: A Study of Antibody Recognition in a Dot Immunoassay for Calmodulin, Calcineurin (β-subunit), and S100B

A simple, sensitive and widely applicable ELISA for S100B: Methodological features of the measurement of this glial protein

“…S100B is a calcium-binding protein and its calcium-binding status greatly affects its antibody recognition (Gonçalves et al, 1997). Previously, we showed that the monoclonal antibody (SH-B1, from Sigma) used for capture in this assay is not affected by the presence of calcium (Gonçalves et al, 1997), but the secondary peroxidase-conjugated anti-S100 antibody (from DAKO), used for detection, had its recognition activity improved by Ca 2+ addition (Green et al, 1997;Tramontina et al, 2000). Conversely, calcium-chelators improve the antigen recognition in the immunoassay with Sangtec antibodies (Tort et al, 2003).…”

Biological and methodological features of the measurement of S100B, a putative marker of brain injury

“…The calcium-binding status of the S100B greatly affects its antibody recognition [106]. Monoclonal antibody (SH-B1, from Sigma) used for capture in our and other assays is not affected by the presence of calcium [106], but the recognition activity of the secondary peroxidase-conjugated anti-S100 antibody (from DAKO), used for detection, is improved by Ca 2+ addition [37,93]. However, the S100B recognition by the non-conjugated polyclonal anti-S100 antibody from DAKO was not affected by the presence or absence of calcium [54].…”

Circulating Biomarkers in Malignant Melanoma

“…However, it is unclear if these antibodies react equally with each complex. In addition, calcium-binding status could also alter S100B recognition immunologically [179]. Unfortunately, methods differ in analytic sensitivity which limits inter-laboratory comparison [180,181].…”