Calcium mediated DNA binding in non-lamellar structures formed by DOPG/glycerol monooleate

Kanjaková, Nina Královič; Hubčík, Lukáš; Búcsi, Alexander; Klacsová, Mária; Combet, Sophie; Teixeira, J.; Martínez, Juan Carlos; Uhrı́ková, Daniela

doi:10.1016/j.chemphyslip.2021.105118

Cited by 1 publication

(3 citation statements)

References 95 publications

(121 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Polarised light microscopy (Figure S4A,B) and SAXS identify predominantly multilamellar structures in Curosurf ® . The repeat distance d~7.8 nm at 40 • C is less than previously reported d~8.4-9.4 nm [4,25,32], which we attribute to the natural origin of the surfactant. However, it is markedly higher than the d values~6.5-6.7 nm reported for phosphatidylcholines in the fluid L α phase [39,54].…”

Section: Discussioncontrasting

confidence: 80%

“…The temperature Tm of the gel-to-fluid phase transition was derived from the position of the maximum, Tm = 28.1 ± 0.3 • C. The enthalpy ∆H = 18.0 ± 1.5 J/g and the phase transition width FWHM~12.5 • C were assessed from the values of the first and third scans (heating-cooling-heating regimen). The data obtained correspond well to the reported values [31][32][33]. The effect of BUD on the thermal behaviour of Curosurf®includes lowering the transition temperature (Tm) and decreasing the latent heat of the transition from gel to fluid.…”

Section: Thermodynamic Parameterssupporting

confidence: 87%

“…In fact, the presence of double bonds in the lipid acyl chains results in a significant drop of Tm, for example, −2.5 ± 2.4 • C for POPC (16:0/18:1PC) or ~−18 • C for PLPC (16:0/18:2PC) [50]. The phase transition temperature Tm = 28.1 ± 0.3 • C and the enthalpy ∆H = 18.0 ± 1.5 J/g derived from DSC thermograms of Curosurf ® ULVs are in accordance with values of Tm = 27.3 ± 0.5 • C and the enthalpy ∆H = 21.1 ± 3.0 J/g determined for MLVs from five independent samples of Curosurf ® (different batches, not expired drug) [32] and the literature [31,33,52]. Differences in thermodynamic parameters (Tm, DH and the width, FWHM) were reported for ULVs and MLVs of saturated diacylphosphatidylcholines [50,53], in general, with lower DH detected for ULVs.…”

Section: Discussionsupporting

confidence: 74%

See 2 more Smart Citations

Thermodynamic and Structural Study of Budesonide—Exogenous Lung Surfactant System

Keshavarzi,

Asi Shirazi,

Korfanta

et al. 2024

IJMS

View full text Add to dashboard Cite

The clinical benefits of using exogenous pulmonary surfactant (EPS) as a carrier of budesonide (BUD), a non-halogenated corticosteroid with a broad anti-inflammatory effect, have been established. Using various experimental techniques (differential scanning calorimetry DSC, small- and wide- angle X-ray scattering SAXS/WAXS, small- angle neutron scattering SANS, fluorescence spectroscopy, dynamic light scattering DLS, and zeta potential), we investigated the effect of BUD on the thermodynamics and structure of the clinically used EPS, Curosurf®. We show that BUD facilitates the Curosurf® phase transition from the gel to the fluid state, resulting in a decrease in the temperature of the main phase transition (Tm) and enthalpy (ΔH). The morphology of the Curosurf® dispersion is maintained for BUD < 10 wt% of the Curosurf® mass; BUD slightly increases the repeat distance d of the fluid lamellar phase in multilamellar vesicles (MLVs) resulting from the thickening of the lipid bilayer. The bilayer thickening (~0.23 nm) was derived from SANS data. The presence of ~2 mmol/L of Ca2+ maintains the effect and structure of the MLVs. The changes in the lateral pressure of the Curosurf® bilayer revealed that the intercalated BUD between the acyl chains of the surfactant’s lipid molecules resides deeper in the hydrophobic region when its content exceeds ~6 wt%. Our studies support the concept of a combined therapy utilising budesonide—enriched Curosurf®.

show abstract