Calcium Localized in Odontogenic Cells of Rat Mandibular Teeth by the Glyoxal Bis (2-Hydroxyanil) Method

Abstract

SYNOPSIS IN INTERLINGUA LE LOCALISATION DE CALCIUM IN CELLULAS ODONTOGENE DE DENTES MANDIBULAR DEL RATTO PER MEDIo DEL METHODO A GLYOXAL-BIS(2-HYDROXYANIL).-Blocos fresc de mandibula, de un spissitate de 1 mm, esseva obtenite ab rattos sugente e tincturate durante 16 horas in un solution de glyoxal-bis(2-hydroxyanil) (GBHA) e postea dishydratate in ethanol, acclarate in xyleno, includite in paraffin, sectionate a un spissitate de 7ju, e affixate per pression digital a albuminisate lamellas de vitro. Le lamellas esseva immergite in un solution de Na2CO3 e KCN pro assecurar specificitate pro calcium e contratincturate con blau methylenic. Granulos rubie de Ca-GBHA esseva localisate in le cytoplasma de adamantoblastos e cementoblastos e in le processes odontoblastic. Calcium in apatite non esseva tincturate.A method for localizing intracellular calcium using glyoxal bis(2-hydroxyanil) (GBHA) was recently developed in this laboratory.' By staining fresh osseous tissues with GBHA, calcium was demonstrable in cytoplasm and processes of osteoblasts, osteocytes, as well as in cytoplasm of periosteal cells and epiphyseal chondrocytes. Using the same GBHA staining procedure, an attempt was made in this investigation to localize calcium in cells of unfixed developing rat teeth.No reports were found that describe calcium in cytoplasm of ameloblasts, odontoblasts, and cementoblasts. However, Watt2 has proposed that calcium may be secreted by the odontogenic cells during tooth formation and by the osteogenic cells during bone formation. His secretary hypothesis was not based on actual observation of calcium in these cells, but merely deduced from his observations on the proximity of the early intercellular calcium deposits to cells.The present report shows calcium as red Ca-GBHA granules in cells that are responsible for formation of enamel, cementum, and dentin.

A model for transcellular transport of calcium based on membrane fluidity and movement of calcium carriers within the more fluid microdomains of the plasma membrane

“…Histochemical studies have not added much to our knowledge of calcium localization: in fact, they have yielded inconclusive and sometimes contradictory findings. The results of the widely cited paper by Kashiwa and Sigman [13] on the presence of calcium in ameloblasts and other cells are difficult to interpret. These authors reported the calcium demonstrated by their methods to be in granular form.…”

“…It was stated by Munhoz and Leblond [! l], in alluding to the paper by Kashiwa and Sigman [13], that if calcium were present in the cells but not detectable in radiographs after 45Ca injection, the likely explanation would be that the cell calcium was in a soluble form and that the soluble form might be the calcium ion. However, the vast body of literature on the role of calcium in cells that has appeared since the Munhoz and Leblond paper (see refs.…”

Histochemical Localization of Calcium in the Enamel Organ of Rat Incisors in Early-Stage Amelogenesis

“…Early histochemical studies by Kashiwa and Sigman [1966] localized granular GBHA reactions in the ameloblast of mandibular molar tooth germs of rats and demonstrated the presence of calcium in these cells. In their studies, however, the drastic treatment of tissues did not allow an exact correlation of GBHA reactions with his tology at the cellular level.…”

“…1985: Chen el al.. 1986: Kogaya and Furuhashi. 1986]: the glyoxal bis(2-hydroxyanil) (GBHA) staining method [Kashiwa and Sigman. 1966], and X-ray microanalysis [Boyde and Reith.…”

Banding Patterns in rat incisor enamel stained by histochemical complexing methods for calcium

“…Glyoxal bis(2-hydroxyanil) (GBHA) has been used to demonstrate soluble and insoluble caIcium salts in tissue sections (Kashiwa and Atkinson, 1962;Kashiwa and House, 1964;Kashiwa, 1966;Kashiwa and Sigman, 1966). More recently, GBHA was used to demonstrate effectively the enamel maturation pattern on entire, intact teeth (Takano et al, 1982b).…”