Calcium ions as a mediator in GnRH action on gonadotropin release in the common carp (Cyprinus carpio L)

Mikołajczyk, T.; Weil, Claudine; Epler, P.; Breton, Bernard

doi:10.1051/rnd:19900403

Cited by 15 publications

(3 citation statements)

References 18 publications

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“…The enzymatic dispersion of the glands was performed with collagenase H (Boehringer Mannheim, Germany) according to the method of Weil et al (1986) for trout and adapted to carp by Mikolajczyk et al (1990), as described elsewhere (SokolowskaMikolajczyk et al, 2005). After dispersion the cells were resuspended in a pre-incubation medium and transferred into two 96-well microplates (Nunc A/S Denmark), approximately 5 × 10 4 cells in each well.…”

Section: Methodsmentioning

confidence: 99%

Steroid effects on the in vitro LH secretion from pituitary cells of sexually immature carp (Cyprinus carpio L.) under the influence of naltrexone and morphine

Sokolowska-Mikolajczyk¹,

Socha²,

Epler³

2010

CZECH J ANIM SCI

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Dispersed cells from sexually immature carp (footlings) pituitaries were exposed to estradiol (E2) or testosterone (T) (both at 3 × 10–8 M) in the presence of opioid receptor antagonist naltrexone (10–8 or 10–6 M) and/or agonist – morphine (10–8 or 10–6 M). Naltrexone alone at 10–6 M increased the LH level as compared with the control. Morphine (10–8 or 10–6 M), T and E2 had no influence on LH levels. The combination of T with naltrexone (10–8 M) stimulated LH release if compared with the control or with T alone. Morphine (both concentrations) with T caused significantly higher LH secretion than the control medium and T alone. Estradiol with naltrexone (10–8 and 10–6 M) had no influence on LH concentration. In media with E2 and morphine (10–8 M) LH levels were higher than in the control and estradiol alone. The results show that in common carp sex steroids affect the response of pituitary cells to opioid agonist or antagonist giving an evidence on the role of steroids in LH release mediated by the opioid system.

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Section: Methodsmentioning

confidence: 99%

Steroid effects on the in vitro LH secretion from pituitary cells of sexually immature carp (Cyprinus carpio L.) under the influence of naltrexone and morphine

Sokolowska-Mikolajczyk¹,

Socha²,

Epler³

2010

CZECH J ANIM SCI

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“…In vivo experiments in tilapia also showed that GnRHa given at doses higher than 10 pg/kg resulted in a reduced response after 3h (Gissis et al 1991). Diminished response to sGnRH at doses higher than 100 nM was reported in perifused carp pituitary cells (Mikolajczyk et al 1990a). Refractoriness of goldfish pituitary fragments was achieved by prolonged exposure to 100 nM sGnRH, but could be circumvented by Ca 2 + ionophore.…”

Section: Discussionmentioning

confidence: 92%

Intracellular mediation of GnRH action on GTH release in tilapia

Levavi‐Sivan

Yaron

1993

Fish Physiol Biochem

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The objective of the present study was to confirm previous results on the mediation of GnRH signal in tilapia by providing evidence from experiments in cultured pituitary cells and from perifusion experiments using a GnRH-antagonist. After 4 days in culture under identical conditions, cells taken from pituitaries of fish maintained at 26°C were more sensitive to GnRHa ([D-Ala(6), Pro(9)-NEt]-LHRH) than those taken from fish maintained at 19°C. Cells from female pituitaries were more responsive than those from males. taGTH release in culture was augmented by Ca(2+) ionophore (A23187; 1-100 μM) or ionomycin (0.02-10 μM). The response of perifused pituitary to GnRH was reduced by nimodipine (1-10 μM) indicating that Ca(2+) influx via voltage-sensitive Ca(2+) channels is involved in the stimulation of GTH release. Activation of protein kinase C by OAG (1-oleyl-2-acetyl glycerol; 0.16-160 μM) or TPA (1-O-tetra-decanoyl phorbol-13-acetate; 1.25-125 nM) resulted in a dose-dependent stimulation of taGTH release from cultured cells. Arachidonic acid (0.33-330 μM) also augmented the release of taGTH from the culture. Four sequential pulses of sGnRH (100 nM) at 2h intervals resulted in surges of taGTH release from perifused pituitary fragments; the surges were similar in magnitude with no signs of desensitization. Sequential stimulation with graded doses of sGnRH (0.1 nM to 1 μM) in the presence of GnRH-antagonist ([Pro(2,6), Trp(3)]-GnRH) resulted in an attenuation of taGTH release. However, the GnRH-antagonist did not alter the pattern of forskolin-stimulated GTH release, indicating that forskolin stimulation is exerted at the level of the adenohypophyseal cells. It is concluded that, as in other vertebrates, the transduction of GnRH stimulation of GTH release involves Ca(2+) influx through voltage-sensitive Ca(2+) channels, mobilization of the ion from intracellular sources, arachidonic acid and activation of PKC. Adenylate cyclase-cAMP system us also involved in the mediation but its relationship with other transduction cascades requires further investigations.

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“…The enzymatic dispersion of the pituitary glands and the technique of cell culture were described in detail elsewhere (Mikolajczyk, Weil, Epler, & Breton, 1990;Sokolowska-Mikolajczyk, Socha, Szczerbik, & Epler, 2009). In brief, collected glands were chopped into small pieces and subjected to dispersion for 6-8 h at 20C in the medium containing 0.1% (w/v) collagenase H (Boehringer Mannheim, Germany) and 1% BSA (Sigma-Aldrich, USA).…”

Section: Culture Techniquementioning

confidence: 99%

Interaction between Kisspetin and Dopamine in the Regulation of in vitro LH Release in Prussian carp (Carassius gibelio Bloch, 1782) Females at the Time of Gonad Recrudescence and Spawning Period

Sokolowska-Mikolajczyk¹,

Gosiewski²,

Chyb³

et al. 2020

Turk. J. Fish. Aquat. Sci.

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The aim of the present study was to demonstrate the in vitro response (LH release) of Prussian carp pituitary cells to mammalian kisspeptin in static incubations. Pituitary glands were collected at two periods of the season (at the time of gonad recrudescence or spawning period) from untreated fish or those with dopaminergic system blocked in vivo by injection of pimozide-a dopamine receptor antagonist. LH measurement (by ELISA method) have shown that there was no significant impact of kisspeptin on the level of this gonadotropin at both investigated seasons of the year if pituitary glands for the study were collected from untreated females. In case of fish pre-treated with pimozide there was a significant stimulation of LH release in both investigated seasons. The results demonstrate the direct effect of kisspeptin on LH release from the Prussian carp gonadotropic cells, dependent on the strength of dopamine inhibition on LH secretion.

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Calcium ions as a mediator in GnRH action on gonadotropin release in the common carp (Cyprinus carpio L)

Abstract: Summary ― Collagenase-dispersed carp pituitary cells in a perifusion system were used to study the role of calcium ions in the mechanism of GnRH action on the release of maturational gonadotropin (GtH)

Cited by 15 publications

References 18 publications

Steroid effects on the in vitro LH secretion from pituitary cells of sexually immature carp (Cyprinus carpio L.) under the influence of naltrexone and morphine

Steroid effects on the in vitro LH secretion from pituitary cells of sexually immature carp (Cyprinus carpio L.) under the influence of naltrexone and morphine

Intracellular mediation of GnRH action on GTH release in tilapia

Interaction between Kisspetin and Dopamine in the Regulation of in vitro LH Release in Prussian carp (Carassius gibelio Bloch, 1782) Females at the Time of Gonad Recrudescence and Spawning Period

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