Calcium ionophoretic and apoptotic effects of ferutinin in the human Jurkat T-cell line

Natural daucane esters induces apoptosis in leukaemic cells through ROS production

“…Following treatment of SEM cells with DE-17 for 24 or 48 h, we observed activation of caspase-3 and of the caspase-3 substrate poly (ADP-ribose) polymerase (PARP) (Fig. 6, panel A) in well agreement with the data of Macho et al (2004). Consistent with the Dw mt results described above, DE17 treatment induced activation of caspase-9, the major initiator caspase of the intrinsic (mitochondrial) apoptosis pathway (Fig.…”

“…S9, Supporting information) suggesting a common mechanism for the antiproliferative effects shared by daucane esters. In this context our findings are different from that of Macho et al (2004) who found an increase of ROS (measured with HE) after 6 h of incubation with ferutinin in Jurkat cells. In fact, this effect occurs concomitantly with mitochondrial depolarization indicating that ROS generation are only a consequence of mitochondrial collapse.…”

“…More importantly, despite many papers describing the antiproliferative effects of daucane esters, only one report address in detail the potential mechanism(s) responsible of this activity. In this paper, Macho et al (2004) showed that ferutinin act as a calcium ionophore in the T-leukemia cell line Jurkat and this event lead to a collapse of the mitochondrial membrane potential with consequent http://dx.doi.org/10.1016/j.phytochem.2014.09.001 0031-9422/Ó 2014 Elsevier Ltd. All rights reserved.…”

Sesquiterpenes from aerial parts of Ferula vesceritensis

“…Its chemistry was largely studied. Daucane esters from F. communis and Ferula arrigonii showed antiproliferative activity on human colon cancer lines (Poli et al, 2005) and calcium ionophoretic and apoptotic effects in the human jurkat T-cell line (Macho et al, 2004).…”

Ferutinin stability in human plasma and interaction with human serum albumin

“…It exhibits an antiproliferative effect on estrogen-dependent MCF-7 cells [7] and induces apoptosis in Jurkat cells through a loss in mitochondrial transmembrane potential and an increase in intracellular reactive oxygen species. The calcium mobilization and apoptosis process mediated by ferutinin requires the p-hydroxylation of the benzoyl moiety [8].…”