Calcium influx following fertilization of Urechis caupo eggs

Johnston, Randal N.; Paul, Miles

doi:10.1016/0012-1606(77)90221-4

Cited by 49 publications

(15 citation statements)

References 27 publications

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“…Several lines of evidence from a variety of species indicate that an intracellular Ca 2+ increase is required for oocyte maturation. During oocyte maturation an intracellular Ca 2+ release has been observed in starfish (Dorée et al 1978;Moreau et al 1978), bivalves (Guerrier et al 1993;Osanai 1994, 1995), echiuroids (Johnston and Paul 1977), amphibians (O'Connor et al 1977;Moreau et al 1980;Vilain et al 1980;Wasserman et al 1980), and mouse and hamster oocytes (Carroll et al 1994;Fujiwara et al 1993). Also, inhibiting intracellular Ca 2+ activities inhibits oocyte maturation in starfish , bivalves (Dubé and Guerrier 1982;Guerrier et al 1993), echiuroids (Johnston and Paul 1977), amphibians (Moreau et al 1976;Masui et al 1977), hamsters (Racowsky 1986) and polychaete worms (Meijer 1980).…”

Section: Introductionmentioning

confidence: 99%

“…During oocyte maturation an intracellular Ca 2+ release has been observed in starfish (Dorée et al 1978;Moreau et al 1978), bivalves (Guerrier et al 1993;Osanai 1994, 1995), echiuroids (Johnston and Paul 1977), amphibians (O'Connor et al 1977;Moreau et al 1980;Vilain et al 1980;Wasserman et al 1980), and mouse and hamster oocytes (Carroll et al 1994;Fujiwara et al 1993). Also, inhibiting intracellular Ca 2+ activities inhibits oocyte maturation in starfish , bivalves (Dubé and Guerrier 1982;Guerrier et al 1993), echiuroids (Johnston and Paul 1977), amphibians (Moreau et al 1976;Masui et al 1977), hamsters (Racowsky 1986) and polychaete worms (Meijer 1980). Conversely, agents that stimulate intracellular Ca 2+ mobilization have been reported to induce oocyte maturation in starfish , bivalves (Schuetz 1975;Guerrier et al 1993) amphibians (Wasserman and Masui 1975b;Moreau et al 1976;Schorderet-Slatkine et al 1976, and polychaete worms (Meijer 1980).…”

Section: Introductionmentioning

confidence: 99%

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The role of Ca 2+ in progesterone-induced germinal vesicle breakdown of Xenopus laevis oocytes: the synergic effects of microtubule depolymerization and Ca 2+

Duesbery

Masui

1996

Development Genes and Evolution

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By monitoring (45)Ca(2+) influx and efflux from oocytes a transient increase followed by a transient decrease in the Ca(2+)-content of progesterone-treated oocytes was observed. Chelation of intracellular Ca(2+) with EGTA or BAPTA-type buffers inhibited progesterone-induced GVBD. Buffers with a mid-range Kd (∼1.5 μM) were most effective in inhibiting GVBD whereas buffers with a Kd above or below this value were less effective. These observations indicate that intracellular Ca(2+), probably in the form of a localized release, is required for progesterone-induced oocyte maturation. However, Ca(2+) alone was insufficient to induce GVBD. When the effects of nocodazole and taxol upon this Ca(2+)-requirement were tested, we observed that taxol-induced microtubule polymerization not only delayed progesterone-induced GVBD but also completely inhibited it in combination with BAPTA-AM. Conversely, nocodazole-induced microtubule depolymerization in combination with ionophore A23187 not only accelerated progesterone-induced GVBD, but also induced GVBD in the absence of progesterone. The combined treatment of oocytes with nocodazole and InsP3, or with cold treatment and ionophore A23187 also induced GVBD in the absence of progesterone. Thus, Ca(2+) and microtubule depolymerization synergistically promote GVBD. In both nocodazole- and cold-treated oocytes, the GV was displaced to the periphery of the oocyte and underwent GVBD when treated with A23187. However, when the GV was displaced to the cortex by a centrifugal force under conditions that would not cause microtubule depolymerization and the oocyte was treated with A23187, oocytes did not undergo GVBD.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The role of Ca 2+ in progesterone-induced germinal vesicle breakdown of Xenopus laevis oocytes: the synergic effects of microtubule depolymerization and Ca 2+

Duesbery

Masui

1996

Development Genes and Evolution

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“…Furthermore, a role of intracellular calcium (Ca;') in the egg has been generally postulated concerning the mechanism of the electrical response, since it has been well established that the spermatozoon, at fertilization, drastically increases (Ca;+). That increase is usually mediated by Ca+ + release either from intracellular stores exclusively Steinhardt et al, , 1977Ridgway et al, 1977;Cuthberson et al, 1981;and review in Jaffe, 19831 or from both intra and extracellular sources [Johnston and Paul, 1977;Finkel and Wolf, 1980;Dub6 and Guerrier, 1982;and review in Jaffe, 19831. In this context, we should outline the case of the "hamster model," which is particularly interesting since it associates the participation of both intra and extracellular Ca + + sources with an electrical response of the fertilized egg membrane somewhat comparable to that we detected in crabs. Indeed, the transient periodic hyperpolarizations of the hamster egg are due to Ca+ + activated K+ conductance and are superimposed on a gradual hyperpolarizing shift of the resting potential.…”

Section: Discussionmentioning

confidence: 99%

Fertilization in crabs: IV. The fertilization potential consists of a sustained egg membrane hyperpolarization

Goudeau

1985

Gamete Research

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The electrophysiological properties of immature and mature oocytes of two crabs were analyzed. Growing immature oocytes of Carcinus maenas and fully grown immature oocytes of Maia squinado had essentially K+ dependent resting potentials, Em, of −61 ∓ 1 mV, n=19, and −67.3 ± 0.5 mV, n=68, respectively. Fully grown immature oocytes of Carcinus maenas showed an Em of −40 ± 1.5 mV, n=19, that was k+ and Cl− dependent. In mature oocytes of both species, the plasma membrane became exclusively permeable to cl− and the Em attained–41 ± 1 mV, n=49 and −34 ± 1.5 mV, n=27 for Carcinus maenas and Maia squinado, respectively. After in vitro insemination, a dramatic increase in egg membrane permeability to K+ was observed. This instantaneously caused a sustained hyperpolarization constituting, for these crabs, the fertilization potential. We observed that concurrently with this electrical response to fertilization, sperm reinitiated the oocyte meiotic maturation previously arrested at the first metaphase. The triggering mechanism of the fertilization potential as well as the possible occurrence of a physiological polyspermy are discussed.

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“…In the case of protostomes, Ca 2+ influx from the outside of the mollusk oocyte has a major contribution to the increase of intracellular Ca 2+ in the fertilized eggs [53][54][55][56]. On the other hand, the Ca 2+ increase in the deuterostome eggs at fertilization has two components: (i) the Ca 2+ release from the internal stores and (ii) the Ca 2+ influx at the entire cortex as 'cortical flash' shortly after the arrival of the fertilizing spermatozoon on the surface.…”

Section: Plural Ca 2+ Changes Proceed In An Oocyte During Fertilizationmentioning

confidence: 99%

“…The Ca 2+ increase at fertilization in Annelida has been studied in C. pergamentaceus, Urechis caupo, and Pseudopotamilla occelata [34,53,[60][61][62]. In C. pergamentaceus and P. occelata, fertilization takes place at metaphase I of the meiotic division.…”

Section: A Unique Ca 2+ Change In the Fertilized Eggs Of Annelidamentioning

confidence: 99%