Calcium-induced inhibition of taurine transport in brush-border membrane vesicles from rabbit small intestine

Miyamoto, Y.; Kulanthaivel, Palaniappan; Ganapathy, Vadivel; Whitford, Gary M.; Leibach, Frederick H.

doi:10.1016/0005-2736(90)90294-x

Cited by 11 publications

(4 citation statements)

References 23 publications

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“…Indeed, permeabilizing duodenal cells to calcium using the ionophore A23187 dramatically reduced Jms and Jnet, without altering Jsm, revealing that an important fraction of total Lalanine transport was depressed by a rise in cytosolic calcium. Although the literature on the regulation of amino acid transport by calcium is scarce, the inhibition of L-alanine and glucose uptake across brush-border vesicles from rabbit small intestine by intravesicular calcium loading has been reported by Miyamoto et al (1990). Similar findings have been observed for several Na + -dependent carriers in rat small intestine vesicles preloaded with a high calcium concentration (Fondacaro and Madden, 1984).…”

Section: Discussionsupporting

confidence: 52%

Regulation of L-alanine transport systems A and ASC by cyclic AMP and calcium in a reptilian duodenal model

Gómez

Medina

Ramírez

et al. 2003

Journal of Experimental Biology

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SUMMARYThe regulation of neutral amino acid transport by cyclic AMP (cAMP) and calcium across the isolated duodenum of the lizard Gallotia gallotihas been studied under short-circuit conditions. Active L-alanine transport was stimulated by forskolin, theophylline and dibutyryl cyclic AMP (db-cAMP). All these agents increased transmural potential difference (PD) and short-circuit current (Isc) in a manner consistent with the activation of a chloride secretory pathway. Both forskolin and theophylline increased intracellular cAMP levels in the lizard duodenal mucosa. Addition of calcium ionophore A23187 rapidly reduced mucosa-to-serosa L-alanine fluxes and diminished net L-alanine transport. Despite the reduction of alanine fluxes by A23187, transepithelial PD and Iscvalues were increased by the ionophore. Analyses of the responses of isolated transport pathways indicated that the Na+-independent L-alanine transport system was unaffected by db-cAMP or calcium ionophore. By contrast,Na+-dependent transport activities were profoundly modified by these agents. Thus, while system A [α-methylamino-isobutiric acid(MeAIB)-transporting pathway] was stimulated by increased calcium, system ASC activity was nearly abolished. Calcium ionophore also potentiated the electrogenic response of system A. Forskolin strongly stimulated system ASC activity but left system A activity unchanged. Activation of system ASC by forskolin was clearly electroneutral, as pre-incubation of the tissues with the chloride channel blocker diphenylamine-2-carboxilic acid (DPC) completely prevented forskolin-induced transepithelial electrical responses. It is concluded that intracellular messengers cAMP and calcium oppositely modulate active Na+-dependent L-alanine transport in the lizard intestine. The different sensitivity exhibited by individual transport pathways may well account for the changes observed in overall alanine transport.

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Section: Discussionsupporting

confidence: 52%

Regulation of L-alanine transport systems A and ASC by cyclic AMP and calcium in a reptilian duodenal model

Gómez

Medina

Ramírez

et al. 2003

Journal of Experimental Biology

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“…The peptide transporter1 (PEPT1) uses a proton electrochemical gradient as the driving force, and its acid‐loading activity requires an apical Na + /H + exchangers or an anion exchangers to reduce epithelial acidification 3,4 . In addition, as an important intracellular secondary messenger, researchers have confirmed that Ca 2+ regulates many intracellular physiological activities and plays an important role in regulating intestinal nutrient absorption through perfusion techniques in the small intestine of rabbits and mice 5–7 . Intracellular Ca 2+ ([Ca 2+ ] i ) homeostasis is finely controlled by Ca 2+ channels and transporters which may ultimately affect nutrient absorption (Table 1).…”

Section: Introductionmentioning

confidence: 99%

“… 3 , 4 In addition, as an important intracellular secondary messenger, researchers have confirmed that Ca 2+ regulates many intracellular physiological activities and plays an important role in regulating intestinal nutrient absorption through perfusion techniques in the small intestine of rabbits and mice. 5 , 6 , 7 Intracellular Ca 2+ ([Ca 2+ ] i ) homeostasis is finely controlled by Ca 2+ channels and transporters which may ultimately affect nutrient absorption (Table 1 ).…”

Section: Introductionmentioning

confidence: 99%

Ion channels and transporters regulate nutrient absorption in health and disease

Lu,

Luo,

et al. 2023

J Cellular Molecular Medi

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Ion channels and transporters are ubiquitously expressed on cell membrane, which involve in a plethora of physiological process such as contraction, neurotransmission, secretion and so on. Ion channels and transporters is of great importance to maintaining membrane potential homeostasis, which is essential to absorption of nutrients in gastrointestinal tract. Most of nutrients are electrogenic and require ion channels and transporters to absorb. This review summarizes the latest research on the role of ion channels and transporters in regulating nutrient uptake such as K+ channels, Ca2+ channels and ion exchangers. Revealing the mechanism of ion channels and transporters associated with nutrient uptake will be helpful to provide new methods to diagnosis and find potential targets for diseases like diabetes, inflammatory bowel diseases, etc. Even though some of study still remain ambiguous and in early stage, we believe that ion channels and transporters will be novel therapeutic targets in the future.

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“…Absorption of taurine in the intestine occurs via a transport system that is specific for taurine and other ,/-amino acids (23). The characteristics of the taurine transporter expressed in the intestinal brush border membrane have been studied in detail (24)(25)(26)(27)(28).…”

Section: Introductionmentioning

confidence: 99%

Regulation of taurine transport by Escherichia coli heat-stable enterotoxin and guanylin in human intestinal cell lines.

Brandsch¹,

Ramamoorthy²,

Marczin³

et al. 1995

J. Clin. Invest.

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The human colon carcinoma cell lines Caco-2 and HT-29 take up on taurine uptake. In accordance with this suggestion, treatment of Caco-2 cells with cholera toxin, which elevated intracellular cAMP levels, was found to inhibit taurine uptake. The steady state levels of the taurine transporter mRNA transcripts were not altered as a result of STa treatment.Studies with Caco-2 cells grown on permeable filters revealed that STa acts from the apical side. The taurine uptake from the apical side was inhibited by STa, but the taurine uptake from the basolateral side remained unaffected. It is suggested that the activity of the intestinal taurine transporter may be regulated by protein kinase A at a posttranslational level and that the intestinal absorption of taurine may be impaired during infection with enterotoxigenic strains of E. coli. (J. Clin. Invest. 1995. 96:361-369.)

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Calcium-induced inhibition of taurine transport in brush-border membrane vesicles from rabbit small intestine

Cited by 11 publications

References 23 publications

Regulation of L-alanine transport systems A and ASC by cyclic AMP and calcium in a reptilian duodenal model

Regulation of L-alanine transport systems A and ASC by cyclic AMP and calcium in a reptilian duodenal model

Ion channels and transporters regulate nutrient absorption in health and disease

Regulation of taurine transport by Escherichia coli heat-stable enterotoxin and guanylin in human intestinal cell lines.

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