Calcium hydroxide root canal sealers: evaluation of pH, calcium ion concentration and conductivity

Silva, L. A. Bezerra da; Leonardo, Mário Roberto; Silva, R. S. da; Assed, Sada; Guimarães, Luiz Fernando

doi:10.1046/j.1365-2591.1997.00079.x

Cited by 27 publications

(20 citation statements)

References 18 publications

(14 reference statements)

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“…The antimicrobial activity of calcium hydroxide-based materials such as Sealapex and Sealer 26 may be related to ionization with subsequent release of hydroxide ions and an increase of pH levels, creating an unfavorable environment for microbial growth (10,27).…”

Section: Discussionmentioning

confidence: 99%

In vitro antimicrobial activity of endodontic sealers, MTA-based cements and Portland cement

et al. 2007

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Section: Discussionmentioning

confidence: 99%

In vitro antimicrobial activity of endodontic sealers, MTA-based cements and Portland cement

et al. 2007

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“…The antimicrobial activity of calcium hydroxide has been reported by the ability of ionization and releasing hydroxyl ions, raising the pH levels, creating an unfavorable environment for microbial growth [19][20] .…”

Section: Discussionmentioning

confidence: 99%

Evaluation of cariogenic antibacterial activity of mineral trioxide aggregate and Portland cement

Júnior

Sobral

Sampaio

et al. 2015

RGO, Rev. Gaúch. Odontol.

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ObjectiveThe objective of this study was to evaluate the antimicrobial activity of Mineral trioxide aggregate e Portland cement against some selected cariogenic bacteria. Methods Wells were made of approximately 0.5 mm in diameter, in solid culture media and immediately filled with cement. Twelve samples of each material were obtained for the realization of the agar diffusion method. These samples were tested with Streptococcus mutans (ATCC 700610), Enterococcus faecalis (ATCC 29212), Lactobacillus acidophilus (UFV) and Lactobacillus casei (UNICAMP). Petri plates containing Tripticase soy Agar (TSA) were used for the growth of Streptococcus mutans and Enterococcus faecalis, and plates of Man Rogosa & Sharpe Agar (MRS) for the Lactobacillus acidophilus and Lactobacillus casei. After 48 hours of incubation, it was made the measurement of inhibition halos with the aid of manual caliper. ResultsThe diameters obtained to MTA (2.50 ± 0.00;2,92 ± 0.63; 2.58 ± 0.38), PC (1.17 ± 0.29; 2.00 ± 0.43; 1.33 ± 0.14) and calcium hydroxide cement (3.83 ± 0.29; 3.00 ± 0.00 ± 2.58 ± 0.72) ResultadosOs diâmetros obtidos do MTA (2.50 ± 0.00; 2,92 ± 0.63; 2.58 ± 0.38), CP (1.17 ± 0.29; 2.00 ± 0.43; 1.33 ± 0.14) e cimento de hidróxido de cálcio (3.83 ± 0.29; 3.00 ± 0.00 ± 2.58 ± 0.72) foram expressos em milímetros e submetidos ao teste de Tukey (p<0.05). Conclusão METHODSThe tested materials were: MTA (Angelus Soluções Odontológicas, Londrina, Brasil), Portland cement (CP II -F32, ITAPESSOCA AGRO-INDUSTRIAL S.A., Goiana, Brasil) and calcium hydroxide paste (Reagen TM).The antimicrobial activity of the materials was evaluated by the Agar diffusion method against four different strains.The strains used for analysis were Streptococcus mutans (ATCC 700610), Enterococcus faecalis (ATCC 29212), Lactobacillus acidophilus and Lactobacillus casei isolated by the Laboratory of Microbiologic Science (Federal University of Viçosa -MG (UFV) and University State of Campinas -UNICAMP, SP, Brazil), respectively.Of each bacterial culture tested, it was held a concentration suspension of approximately 5x108 of colonies per mL, corresponding to 0.5 of Mc Farland em range of sterile medium Trypticase Soy Browth (TSBMerck). The Streptococcus mutans and Enterococcus faecalis suspensions were inoculated with spread plate in the middle of Sodium Tioglicolate (Merck) culture and Lactobacillus and Lactobacillus acidophilus casei were inoculated in the culture medium Man Rogosa & Sharpe Agar (MRS-HIMEDIA). Subsequently, the plates were incubated at 35 ± 1°C for 24/48h and the halos of inhibition were expressed in millimeters, using a caliper manual.A total of 12 plates were employed, each microorganism was tested in triplicate. Three cavities, measuring 4mm in diameter were made in each agar plate using a copper puncher and then completely filled with freshly manipulated product to be tested.Positive (CaOH) and negative controls (sterile growing medium) were tested using the same methodology and under the same conditions of the experiment.All cult...

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“…The pH of Apexit (Ivoclar Vivadent, Schaan, Liechtenstein), a commercial CH-based endodontic sealer paste, is reported to be 10.5 (21). However, its pH did not seem to be adequate to provide in vitro inhibition of Candida albicans, Staphylococcus aureus, and S. mutans (22).…”

Section: Discussionmentioning

confidence: 99%

Effects of a Novel Hydration Accelerant on the Biological and Mechanical Properties of White Mineral Trioxide Aggregate

Ji¹,

Wu²,

Hsieh³

et al. 2011

Journal of Endodontics

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Calcium hydroxide root canal sealers: evaluation of pH, calcium ion concentration and conductivity

Cited by 27 publications

References 18 publications

In vitro antimicrobial activity of endodontic sealers, MTA-based cements and Portland cement

In vitro antimicrobial activity of endodontic sealers, MTA-based cements and Portland cement

Evaluation of cariogenic antibacterial activity of mineral trioxide aggregate and Portland cement

Effects of a Novel Hydration Accelerant on the Biological and Mechanical Properties of White Mineral Trioxide Aggregate

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