2011
DOI: 10.4025/actascianimsci.v33i1.10686
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Calcium-free and low-calcium water production for aquaculture research

Abstract: ABSTRACT. The present work aimed to obtain a mathematical expression to calculate the amount of EDTA required to produce calcium-free and low-calcium water ([Ca 2+ ] < 5 mg L -1 ). Initially, a pilot test was carried out to confirm the effectiveness of EDTA as a chelating agent of waterborne calcium and to evaluate its effect on water pH. Five application rates of Na-EDTA (25, 50, 75, 100 and 125 mg L -1 ) were tested. The concentration of calcium in water was determined initially (before the application … Show more

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