Calcium Enhances the Sensitivity of Immunofluorescence for Pemphigus Antibodies

Matis, Wendy L.; Anhalt, Grant J.; Díaz, Luis A.; Rivitti, Evandro A.; Martins, Camila Bertini; Berger, R.

doi:10.1111/1523-1747.ep12471587

Cited by 54 publications

(17 citation statements)

References 12 publications

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“…[1112] It has been shown that a calcium-containing buffer solution enhances the sensitivity of IIF in pemphigus group of patients by two or more doubling dilutions. [13]…”

Section: Immunofluorescence Testsmentioning

confidence: 99%

Advanced diagnostic techniques in autoimmune bullous diseases

2017

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“…[1112] It has been shown that a calcium-containing buffer solution enhances the sensitivity of IIF in pemphigus group of patients by two or more doubling dilutions. [13]…”

Section: Immunofluorescence Testsmentioning

confidence: 99%

Advanced diagnostic techniques in autoimmune bullous diseases

2017

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“…24 Como em outras formas de pênfigo, um elemento essencial no diagnóstico do FS é a detecção de anticorpos intercelulares (AI) circulantes, classicamente detectados por imunofluorescência indireta (IFI). 25 27 através da IFI, utilizando como substrato pele humana normal, realizaram estudo soroepidemiológico em foco endêmico de FS e constataram ausência de autoanticorpos antiepiteliais do FS em familiares consanguíneos, coabitantes e vizinhos dos doentes moradores em áreas endê-micas. Cunha et al, 28 estudando soros de 23 pacientes com FS por seu padrão de fluorescência contra pele humana normal por IFI, constataram que 83% dos pacientes reagiram a antígeno(s) intercelular(es) presente(s) na camada superficial da epiderme, em distribuição idêntica àquela do padrão da Dsg1 por anticorpo monocronal AE23.…”

Section: Imunofluorescênciaunclassified

Dermatoses bolhosas auto-imunes

Cunha

Barraviera²

2009

An. Bras. Dermatol.

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Resumo: Dermatoses bolhosas autoimunes são doenças cuja manifestação cutânea primária e fundamental consiste em vesículas e bolhas. Classificam-se conforme a localização da bolha, em intraepidérmica e subepidérmica. Os pacientes produzem autoanticorpos contra estruturas específicas da pele detectáveis por técnicas de imunofluorescência, immunobloting e Elisa. Os recentes avanços da biologia molecular e celular têm permitido conhecer esses autoantígenos, contra os quais os pacientes se sensibilizam e que estão localizados na epiderme ou na junção dermoepidérmica. São doenças de baixa incidência, porém de elevada morbidade e por vezes letais. O objetivo deste trabalho é revisar e descrever os progressos nos conhecimentos de quatro doenças vésico-bolhosas autoimunes: pênfigo foliáceo endêmico (fogo selvagem), pênfigo vulgar, penfigóide bolhoso e dermatite herpetiforme. Palavras-chave: Alergia e imunologia; Dermatite herpetiforme; Dermatopatias vesiculobolhosas; Penfigo; Penfigóide bolhoso Abstract: Abstract: Autoimmune bullous dermatoses are diseases in which blisters and vesicles are the primary and fundamental types of skin lesion. Their classification is based on the location of the blister: intraepidermal and subepidermal. Patients produce autoantibodies against self-specific structures of the skin detectable by immunofluorescence techniques, immunoblotting and ELISA. Recent advances in molecular and cellular biology have brought to knowledge these self-antigens, against which patients are sensitized, and which are found in epidermis or in the dermo-epidermal junction. These are low incidence, but high morbidity diseases that may be fatal. The aim of this article is to review and describe the progress of four autoimmune vesiculobullous disorders: endemic pemphigus foliaceous (wild fire), pemphigus vulgaris, bullous pemphigoid and dermatitis herpetiformis. Keyword: Allergy and immunology; Bullous pemphigoid; Dermatitis herpetiformis; Pemphigus; Skin diseases vesiculobullous histológicas comuns e ambos apresentam autoanticorpos da subclasse IgG4, cujo antígeno-alvo é a desmogleína 1, antígeno desmossomal de 160kd.O FS acomete mais frequentemente crianças, adolescentes e adultos jovens que vivem nas áreas rurais de regiões endêmicas, e é comum a ocorrência

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“…3a, b Pemphigus foliaceus. a Indirect immunoperoxidase staining (applying 5 mM CaCl 2 [96]) of human epidermis detects circulating IgG4 autoantibodies with typical, suprabasally enhanced intercellular staining pattern (Brazilian endemic form). ×700.…”

Section: Desmogleinmentioning

confidence: 99%

“…immunofluorescence microscopy, a procedure that is most effective when a calcium-ion-containing buffer is used [96]; indirect immunoperoxidase microscopy can be used to obtain comparable results (Fig. 4a).…”

Section: Desmogleinmentioning

confidence: 99%

Epidermal adhesion molecules and basement membrane components as target structures of autoimmunity

Moll

1998

Virchows Archiv

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Intraepidermal and dermal-epidermal cohesion are of paramount importance for the integrity of the skin. Some constituent molecules of keratinocyte adhesion complexes and basement membrane-associated structures are the targets of antibody-mediated autoimmune reactions that give rise to various (muco-)cutaneous blistering diseases. The current state of our knowledge about these molecules--along with the main clinical, histological, and immunohistochemical features of the corresponding autoimmune diseases and their pathogenetic mechanisms--comprise the subjects surveyed in this review. Among the desmosomal cadherins (desmogleins and desmocollins) that mediate epidermal cell-cell adhesion, it has been demonstrated that desmoglein 1 and desmoglein 3 are the autoantigens of pemphigus foliaceus and pemphigus vulgaris, respectively, both diseases that result in intraepidermal blistering. Further, desmocollin autoantibodies may be involved in IgA pemphigus. Paraneoplastic pemphigus is associated with autoantibodies directed against the desmosomal plaque protein, desmoplakin. Of the constituents of hemidesmosomes, the plaque protein, BP230 (BPAG1), and the collagen-like transmembrane protein, BP180 (BPAG2), are the autoantigens of bullous pemphigoid and pemphigoid gestationis, the manifestations of both of which include subepidermal blistering. Several diseases arise from autoimmune reactions against certain proteins associated with the basement membrane located beneath hemidesmosomes, for example laminin 5 (cicatricial pemphigoid), ladinin (LAD-1; linear IgA disease), uncein, and collagen VII (epidermolysis bullosa acquisita), the last of which is the constituent protein of the anchoring fibrils. Such recent advances in the elucidation of the molecular nature of autoantigens may serve as the basis for the development of novel molecule-based therapeutic strategies.

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Calcium Enhances the Sensitivity of Immunofluorescence for Pemphigus Antibodies

Cited by 54 publications

References 12 publications

Advanced diagnostic techniques in autoimmune bullous diseases

Advanced diagnostic techniques in autoimmune bullous diseases

Dermatoses bolhosas auto-imunes

Epidermal adhesion molecules and basement membrane components as target structures of autoimmunity

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