2014
DOI: 10.1038/nn.3906
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Calcium dynamics in astrocyte processes during neurovascular coupling

Abstract: Enhanced neuronal activity in the brain triggers a local increase in blood flow, termed functional hyperemia, via several mechanisms, including calcium (Ca2+) signaling in astrocytes. However, recent in vivo studies have questioned the role of astrocytes in functional hyperemia because of the slow and sparse dynamics of their somatic Ca2+ signals and the absence of glutamate metabotropic receptor 5 in adults. Here, we reexamined their role in neurovascular coupling by selectively expressing a genetically encod… Show more

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Cited by 245 publications
(256 citation statements)
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“…1E) and a greater number of signals ( Fig. 1D) than endfeet or somata, comparable to previous reports with other calcium indicators and in other brain regions (Shigetomi et al 2013;Gee et al 2014;Kanemaru et al 2014;Otsu et al 2015;Srinivasan et al 2015;Tang et al 2015). A previous study has also detected three different populations of GCaMP6s peaks in astrocytes: single peaks, multi peaks, and plateaus (Bonder and McCarthy 2014).…”
Section: Discussionsupporting
confidence: 91%
“…1E) and a greater number of signals ( Fig. 1D) than endfeet or somata, comparable to previous reports with other calcium indicators and in other brain regions (Shigetomi et al 2013;Gee et al 2014;Kanemaru et al 2014;Otsu et al 2015;Srinivasan et al 2015;Tang et al 2015). A previous study has also detected three different populations of GCaMP6s peaks in astrocytes: single peaks, multi peaks, and plateaus (Bonder and McCarthy 2014).…”
Section: Discussionsupporting
confidence: 91%
“…Ca 2+ fluorescence signals and 2-NBDG fluorescence signals were analyzed in regions of interest (ROIs) covering the somata of oligodendroglial cells. Normalized changes of Rhod-2 fluorescence intensities were calculated as ΔF/F=(F−F0)/F0 (F, fluorescence intensity; F0, baseline intensity) (Otsu et al, 2015). And the relative changes of 2-NBDG fluorescence intensities were calculated as the (F−F0)/S [F, 2-NBDG fluorescence intensity at end time point; F0, baseline 2-NBDG fluorescence intensity; S, time (seconds) from start point of treatment to the end time point of experiment].…”
Section: Intracellular Camentioning
confidence: 99%
“…102 Further recent studies have confirmed that astrocytic, fast Ca 2þ transients that occur in the cell soma, fine processes, and endfeet reliably precede functional hyperemia in adult mice in vivo. 103,104 Thus, it appears that astrocytes from IP 3 R2-knockout mice do continue to engage in Ca 2þ signaling, perhaps due to compensatory upregulation of other IP 3 R isoforms, likely on a spatial and temporal scale that previous studies were unable to detect.…”
Section: Ip 3 Rsmentioning
confidence: 99%