Calcium-dependent effect of Tricolorin A on intestinal and arterial smooth muscle contractility

Sedative, vasorelaxant, and cytotoxic effects of convolvulin from Ipomoea tyrianthina

“…Only at high concentrations (50 g/mL) the response is poorly visualized in endotheliumdenuded aortic rings. Our result is similar to that reported for tricolorin A, which showed a concentration-dependent vasorelaxation of the isolated intact rat aorta, this vascular relaxation was endothelium-dependent and significantly decreased in the presence of nitric oxide synthase and a NO scavenger (Hernández et al, 2008). IT-EM seems to produce their vasorelaxant effect through endothelium derived factors as nitric oxide synthase pathways.…”

“…The jalapin of Ipomoea tyrianthina inhibited contractions on rat ileum (Mirón-López et al, 2007). Tricolorin A, a glycolipid isolated from the jalapin of Ipomoea tricolor, provoked spontaneous contractions of guinea pig ileum (Hernández et al, 2008). The fact that convolvulin (IT-EM) did not inhibit significantly contractions on rat ileum, jejune, and colon can be due to the major polarity of their glycolipids.…”

Cytotoxic Resin Glycosides from Ipomoea aquatica and Their Effects on Intracellular Ca2+ Concentrations

“…The 13 C NMR spectrum ( Table 2) showed signals that could be ascribed to three carboxyl carbons (δ C 175.9, 173.9, 173.6) and five anomeric carbons (δ C 104. 8 14 This result was confirmed by the alkaline hydrolysis of 1, which afforded a water-soluble glycoside acid in the aqueous phase and 2-methylbutyric acid and n-hexanoic acid in the CHCl 3 layer. 11,12 The configuration of 2-methylbutyric acid was determined as S by comparison of its optical rotation value ([α] 27 D +17.6) with that of an authentic sample.…”

“…Chemical investigations on these resin glycosides were initiated in the middle of the 19th century. To date, hundreds of resin glycosides have been reported, some of which exhibit cytotoxic, neuroprotective, sedative, and vasorelaxant activities, and reversal effects on multidrug resistance in both microbial pathogens and mammalian cancer cells. − Notably, several resin glycosides have shown ion-transport activities on Na + , K + , and Ca 2+ ions, , and Ca 2+ influx was proved to be the basis of the contractile effect of tricolorin A, a typical resin glycoside, on the guinea pig ileum . Thus, the effects of resin glycosides on cellular Ca 2+ homeostasis deserve further investigation.…”

Different effects on the tonus of colon and ileum isolated from mouse by resin glycoside (pharbitin) of Pharbitidis Semen

“…Resin glycosides are characteristic ingredients of certain laxative crude drugs such as Mexican Scammony Radix, Orizabae Tuber, and Jalapae Tuber, all of which originate from Convolvulaceae plants [ 1 ]. A resin glycoside of jalapin type from Ipomoea tricolor , provoked spontaneous contractions of guinea pig ileum [ 8 ], however, those from I. tyrianthina resulted in relaxant effects on spontaneous contractions in isolated rat ileum [ 9 ]. The convolvulin type isolated from the Convolvulaceae family induced peristalsis in the small intestine [ 10 ], and that from I. tyrianthina did not inhibit significantly contractions on rat ileum and colon [ 11 ].…”