Calcium‐dependent chloride currents in isolated cells from rat lacrimal glands.

Tmem16A Encodes the Ca2+-activated Cl− Channel in Mouse Submandibular Salivary Gland Acinar Cells

“…Previous reports demonstrate that members of the TMEM16 (4 -7) and BEST (8,(11)(12)(13) gene families encode Ca 2ϩ -activated Cl Ϫ channels with properties much like those found in native tissues (1,(15)(16)(17)(18). TMEM16A was previously detected in a proteomics screen of exosomal proteins isolated from human parotid saliva (32) and in the apical membrane of mouse salivary acinar cells (6), whereas mouse salivary gland acinar cells express transcripts for Best2 (9).…”

“…Both bestrophin and TMEM16 channels have been shown to generate Ca 2ϩ -activated Cl Ϫ currents in vitro that are dependent on physiological intracellular Ca 2ϩ concentrations (4 -6, 8 -10). TMEM16 and BEST Ca 2ϩ -activated Cl Ϫ channels (4 -6, 8, 11-14) share many of the functional and pharmacological properties of the CaCC expressed in native secretory cells (15)(16)(17)(18), unlike the other putative Ca 2ϩ -activated Cl Ϫ channels, i.e. CLCA, CLC3, and TWEETY (19 -22).…”

Microelectrode study on the ionic mechanisms which contribute to the noradrenaline‐induced depolarization in isolated cells of the rabbit portal vein

“…However, the shift of the reversal potential of the noradrenaline-induced responses to positive potentials when Nal and NaNO3 were used in the microelectrodes indicates that our conclusions are likely to be valid. The order of halide permeability (I-> Br-> Cl-) has also been found in rat lacrimal glands cells (Evans & Marty, 1986), cultured Schwann cells (Gray et al, 1984) and in Xenopus laevis oocyte membranes reconstituted into planar bilayers (Young et al, 1984). Moreover the same sequence is found for the channel opened by glycine and y-aminobutyric acid (GABA) in mouse cultured spinal neurones (Bormann et al, 1987).…”

“…In a previous publication we noted the similarity between the calcium-activated chloride conductance increase in rat lacrimal glands (Marty et al, 1984) and the rat anococcygeus muscle (Byrne & Large, 1987b). Interestingly in rat lacrimal gland cells the chloride channel appears to be more permeable to NO3-than to Br- (Evans & Marty, 1986) (Shoemaker et al, 1985), in the ctenophore Mnemiopsis (Stein et al, 1985), rat anococcygeus muscle (Byrne & Large, 1987a), guinea-pig uterus (Coleman & Parkington, 1987), rabbit portal vein (Byrne & Large, 1988b) and the guinea-pig mesenteric vein (van Helden, 1988). In the guinea-pig vas deferens it has been demonstrated that the chloride equilibrium potential is about -25mV (Aickin & Brading, 1982).…”

Effects of excitatory neurotransmitters on Ca2+ channel current in smooth muscle cells isolated from guinea‐pig urinary bladder

“…This suggests that the large outward current observed is due to ion channels which possess both Ca2+-and voltage-sensitivities, e.g. Ca2"-activated Cl--channels (Amedee et al, 1990;Evans & Marty, 1986), Ca2"-activated non-selective cation channels (Inoue & Isenberg, 1990a,b;Loirand et al, 1991). Outward Cs' current through Ca2"-activated K+ channels may also have some contribution.…”