Calcium deficiency modifies polyunsaturated fatty acid metabolism in growing rats

Marra, Carlos Alberto; Alaniz, María J. T. de

doi:10.1007/s11745-000-0609-8

Cited by 15 publications

(23 citation statements)

References 57 publications

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“…An aliquot of 20 ll was taken for protein measurement [10] and the remainder was treated with Folch reactive [11]. After processing the samples according to Folch methodology, an aliquot of the final extract was taken to determine total lipid content gravimetrically [12] on a XP56-Delta Range Mettler Toledo balance. The evaporated residue was used for quantitative phosphorus determination according to Chen et al [13].…”

Section: Lipid Analysesmentioning

confidence: 99%

A Reliable Biomarker Derived from Plasmalogens to Evaluate Malignancy and Metastatic Capacity of Human Cancers

Smith

Lespi

Luca

et al. 2007

Lipids

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Antigen tumor markers employed in monitoring therapeutical approaches are limited by their specificity (Sp) and sensitivity (Se). The aim of this study was to investigate the suitability of a lipid tumor marker derived from ether-linked phospholipids and to compare it with others usually assayed in clinical practice. Complex lipids from normal and pathological breast, lung, and prostate tissue were isolated and analyzed by TLC and c-GLC methods. Results were compared as pooled samples, or by means of the averaged percent changes with respect to the composition observed in the normal tissue of the same patient. Sp, Se, negative-predictive (NPV) and positive- predictive values (PPV) were established for conventional markers and for the proposed lipid-derived marker. Results demonstrated that the content of monoenoic fatty acyl chains was significantly increased in total lipids, phosphatidylethanolamine, and especially in ethanolamine-containing ether lipids of neoplastic tissues with respect to their corresponding normal ones. Major changes were observed in the plasmalogen sub-fraction where the ratio monoenoic/saturated fatty acids can distinguish with high Se normal tissues from either benign or neoplastic tissues from breast, lung, or prostate lesions. Analyses of fatty acyl chains from ethanolamine-containing plasmalogens provided a reliable tumor marker that correlated with high Se and linearity with metastases spreading. This fact may be useful in prognosis of the most frequently observed human cancers.

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Section: Lipid Analysesmentioning

confidence: 99%

A Reliable Biomarker Derived from Plasmalogens to Evaluate Malignancy and Metastatic Capacity of Human Cancers

Smith

Lespi

Luca

et al. 2007

Lipids

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“…Administration of E 2 enhances the VLDL secretion [26,184]. In rats, Ca deficiency modifies the polyunsaturated fatty acid metabolism by reducing liver desaturase activity [107].…”

Section: Fatty Liver-related Diseasesmentioning

confidence: 99%

“…It appears that the elevation of the level of this steroid hormone during the peripartum period has bifunctional effects on fatty liver development and immunosuppression. Hypocalcemia has also been suggested to act as an immunosuppressor by decreasing the production of polyunsaturated fatty acids [107], the precursors for prostaglandins.…”

Section: Fatty Liver-related Diseasesmentioning

confidence: 99%

Relevance of Apolipoproteins in the Development of Fatty Liver and Fatty Liver-Releted Peripartum Diseases in Dairy Cows.

2002

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ABSTRACT. Most metabolic diseases in dairy cows occur during the peripartum period and are suggested to be derived from fatty liver initially developed during the nonlactating stage. Fatty liver is induced by hepatic uptake of nonesterified fatty acids that are released in excess by adipose tissues attributable to negative energy balance. The fatty accumulation leads to impairment of lipoprotein metabolism in the liver, and the impairment in turn influences other metabolic pathways in extrahepatic tissues such as the steroid hormone production by the corpus luteum. Detailed understanding of the impaired lipoprotein metabolism is crucial for elucidation of the mechanistic bases of the development of fatty liver and fatty liver-related peripartum diseases. This review summarizes results on evaluation of lipoprotein lipid and protein concentrations and enzyme activity in cows with fatty liver and those with ketosis, left displacement of the abomasum, milk fever, downer syndrome and retained placenta. Obtained data strongly suggest that decreases in serum concentrations of apolipoprotein B-100, apolipoprotein A-I and apolipoprotein C-III, a reduction in activity of lecithin:cholesterol acyltransferase and induction of haptoglobin and serum amyloid A are intimately related to the development of fatty liver and fatty liver-related diseases. Moreover, determination of the apolipoprotein concentrations and enzyme activity during the peripartum period is useful for early diagnoses of these diseases. KEY WORDS: apolipoprotein, bovine, estrogen receptor, fatty liver, protein kinase C.J. Vet. Med. Sci. 64(4): 293-307, 2002 PREFACE AND SCOPE OF THIS REVIEW Control of metabolic diseases, including ketosis, left displacement of the abomasum (LDA), milk fever and downer cow syndrome are crucially important for modern dairy husbandry, because their occurrence is intimately related to extensive feeding and management of dairy cows and also because high-yielding cows are particularly susceptible to such diseases. Affected cows, even after recovery from those metabolic diseases, have high incidences of reproductive and infectious diseases. Most metabolic diseases occur during the peripartum period (2 to 4 weeks from parturition) and are suggested to be caused by fatty liver (hepatic lipidosis) initially developed during the nonlactating stage. Fatty liver is a condition in which triglycerides (TG) accumulate in the liver. As a logical consequence, the TG accumulation results in impaired metabolism of lipoproteins, most of which are produced by the liver. Lipoproteins have a role in the transport of lipids between the liver and extrahepatic tissues. Impairment of lipoprotein metabolism is linked to disturbances of other metabolic pathways in extrahepatic tissues, for example, the steroid hormone production in the corpus luteum. Studies on impaired lipoprotein metabolism are therefore prerequisite to elucidate the mechanistic bases of the development of fatty liver and fatty liver-related diseases. Lipoproteins consist of lipids a...

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“…Inc., Cambridge, MA, USA) as described elsewhere [37][38][39]. Protein content was determined by the micromethod of Bradford [72] with crystalline BSA as standard.…”

Section: Other Methodologiesmentioning

confidence: 99%

“…Samples were individually dispensed into heparinized tubes and fractionated by centrifugation at 500 g (10 min). Plasmas were immediately processed for calcium determination following the method described elsewhere [37,38] using the procedure of Tomlinson and Dhalla [39]. Nitrite plus nitrate levels were measured according to Verdon et al [40].…”

Section: Sample Collection and Subcellular Fraction Preparationmentioning

confidence: 99%

Lipid Metabolism in Rats is Modified by Nitric Oxide Availability Through a Ca⁺⁺‐Dependent Mechanism

Marra

Nella

Manti

et al. 2007

Lipids

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We studied lipid metabolism and the antioxidant defense system in plasma and liver of rats fed diets supplemented with L(omega)-nitro-L-arginine methyl ester (L-NAME), isosorbide dinitrate (DIS), L-arginine (Arg), or the associations of these drugs. Liver hydroperoxide and thiobarbituric-acid-reactive substance (TBARS) levels were decreased by Arg and increased by L-NAME or DIS treatments. Oxidized glutathione and conjugated dienes were increased by DIS. Nitrate + nitrite levels and serum calcium ([Ca(++)]) were incremented by Arg or DIS and reduced by L-NAME. Superoxide dismutase and catalase activities decreased under Arg treatment, while L-NAME or DIS caused stimulation. Liver high-density lipoprotein (HDL) cholesterol was increased by DIS or NAME (alone or associated with Arg). Free fatty acids and neutral and polar lipids were increased by Arg, L: -NAME, and DIS. However, predominating phospholipid synthesis increased the neutral/polar ratio. Decreased levels of nitric oxide (NO) (low [Ca(++)]) was directly associated with increased fatty acid synthetase, decreased phospholipase A(2), carnitine-palmitoyl transferase, and fatty acid desaturase activities. Raised NO (high [Ca(++)]) inversely correlated with increased phospholipase-A(2) and acyl-coenzyme A (CoA) synthetase and decreased fatty acid synthetase and beta-oxidation rate. Arg or DIS produced changes that were partially reverted by association with L-NAME. Based on these observations, prolonged therapeutical approaches using drugs that modify NO availability should be carefully considered.

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Calcium deficiency modifies polyunsaturated fatty acid metabolism in growing rats

Cited by 15 publications

References 57 publications

A Reliable Biomarker Derived from Plasmalogens to Evaluate Malignancy and Metastatic Capacity of Human Cancers

A Reliable Biomarker Derived from Plasmalogens to Evaluate Malignancy and Metastatic Capacity of Human Cancers

Relevance of Apolipoproteins in the Development of Fatty Liver and Fatty Liver-Releted Peripartum Diseases in Dairy Cows.

Lipid Metabolism in Rats is Modified by Nitric Oxide Availability Through a Ca⁺⁺‐Dependent Mechanism

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Calcium deficiency modifies polyunsaturated fatty acid metabolism in growing rats

Cited by 15 publications

References 57 publications

A Reliable Biomarker Derived from Plasmalogens to Evaluate Malignancy and Metastatic Capacity of Human Cancers

A Reliable Biomarker Derived from Plasmalogens to Evaluate Malignancy and Metastatic Capacity of Human Cancers

Relevance of Apolipoproteins in the Development of Fatty Liver and Fatty Liver-Releted Peripartum Diseases in Dairy Cows.

Lipid Metabolism in Rats is Modified by Nitric Oxide Availability Through a Ca++‐Dependent Mechanism

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Lipid Metabolism in Rats is Modified by Nitric Oxide Availability Through a Ca⁺⁺‐Dependent Mechanism