1990
DOI: 10.1161/01.res.67.1.199
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Calcium current in single cells isolated from normal and hypertrophied rat heart. Effects of beta-adrenergic stimulation.

Abstract: The L-type calcium current was investigated in normal and hypertrophied rat ventricular myocytes as a possible cause of the action potential lengthening that has been reported during hypertrophy. Regulation of the calcium current (lCa) by a ,B-adrenergic agonist (isoproterenol) was also analyzed since P-agonist-induced positive inotropy is less marked in hypertrophied

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Cited by 128 publications
(78 citation statements)
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“…The E max of isoproterenol obtained for sham myocytes was similar to values previously reported for adult rat hearts (36), while the present study is the first report of E max and EC 50 in postinfarction rat myocytes. The maintenance of EC 50 and the decrease in E max in postinfarction myocytes suggest that the reduced effect of isoproterenol on these cells is more likely to be related to a decreased ß-adrenergic receptor density rather than to a change in affinity of the ß-adrenergic receptor.…”
Section: Depressed ß-Adrenergic Response In Postinfarction Myocytessupporting
confidence: 91%
“…The E max of isoproterenol obtained for sham myocytes was similar to values previously reported for adult rat hearts (36), while the present study is the first report of E max and EC 50 in postinfarction rat myocytes. The maintenance of EC 50 and the decrease in E max in postinfarction myocytes suggest that the reduced effect of isoproterenol on these cells is more likely to be related to a decreased ß-adrenergic receptor density rather than to a change in affinity of the ß-adrenergic receptor.…”
Section: Depressed ß-Adrenergic Response In Postinfarction Myocytessupporting
confidence: 91%
“…Male Wistar rats (180-250 g) were anesthetized by intraperitoneal injection of urethane (2 g/kg) and heparin (2.5 mg/kg). Ventricular cardiomyocytes were dispersed using collagenase A (0.255 mg/ml; Boehringer-Mannheim Biochemica, Mannheim, Germany) as previously described (31). At the end of the retrograde perfusion, atria and ventricles were separated, and the isolated ventricular myocytes were resuspended, step by step, in a 1 mM Ca 2ϩ -containing solution.…”
Section: Methodsmentioning
confidence: 99%
“…Previous studies on left ventricular trabeculae (Meszaros & Levai, 1990, 1992Meszaros, 1992) (Kleiman & Houser, 1988;Scamps et al 1990;Bouron, Potreau & Raymond, 1992;Cerbai, Barbieri, Li & Mugelli, 1994) although in mild (Ryder et al 1993) and moderate (Keung, 1989) hypertrophy, an increase in ICa density has been reported. Only in severe cardiac hypertrophy, in which congestion is present, has ICa density been reported to decrease (Nuss & Houser, 1991; Rossner, 1991;Aiba & Creazzo, 1992;Hatem, Sham & Morad, 1994; LeGrand, Hatem, Deroubaix, Couetil & Coraboeuf, 1994;Ming, Nordin, Sirn & Aronson, 1994).…”
Section: Discussionmentioning
confidence: 84%