Calcium Carbonate Formation in the Presence of Biopolymeric Additives

Azulay, David N; Chai, Liraz

doi:10.3791/59638-v

Cited by 3 publications

(3 citation statements)

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“…We used the double-jet method 60 to form crystals in the absence and presence of levan (30 mg/mL). The crystals were collected 7 days after they formed on glass slides that were prepositioned in the bottom of a glass vial (see schematic Figure 1a).…”

Section: Resultsmentioning

confidence: 99%

“…Calcium oxalate (CaOx) was precipitated using the double jet method. 60 In the control experiments, calcium chloride (CaCl 2 , 0.25 mL of 2 mM) and sodium oxalate (Na 2 C 2 O 4 , 0.25 mL of 2 mM) were injected at a 1 mL/h rate using two different syringes into a container vial containing a solution of 0.5 mL of 0.04% sodium azide dissolved in deionized water (resistivity = 18.2 MΩ cm, Wasserlab) (Figure 1a). Sodium azide was used to prevent bacterial growth.…”

Section: Methodsmentioning

confidence: 99%

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A Bacterial Biofilm Polysaccharide Affects the Morphology and Structure of Calcium Oxalate Crystals

Azulay,

Fraenkel,

Chai

2023

Crystal Growth & Design

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Biomineralization describes the process of mineral precipitation from soluble precursors by living organisms. It is sometimes associated with single bacterial cells, for example, the formation of magnetosomes by magnetotactic bacteria, as well as with groups of bacterial cells that form biofilms and precipitate calcium carbonate (CaCO3). Recently, there has been growing evidence connecting isolated bacteria and bacterial biofilms with calcium oxalate (CaOx) formation in kidney stones. Therefore, in this study, we examined the effect of a principal exopolysaccharide bacterial biofilm component on the crystallization of CaOx. We observed that the exopolysaccharide, identified as levan, induced the formation of both octahedral CaOx dihydrate (COD, Weddellite) and pancake-like CaOx monohydrate crystals (COM, Whewellite) in a concentration-dependent manner. A combined analysis of the CaOx crystals that formed in the presence of levan, using scanning electron microscopy, Raman spectroscopy, and X-ray diffraction, indicated that levan affects both the nucleation and the growth of CaOx and that its interaction with CaOx is stereospecific. Given the emerging relation between bacterial biofilms and kidney stones, which are prevalent within approximately 12% of the worldwide population, it is important to decipher the effect of biofilm extracellular polymers on the formation of CaOx crystals as it may assist in the development of future treatments to interfere with kidney stone formation.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

A Bacterial Biofilm Polysaccharide Affects the Morphology and Structure of Calcium Oxalate Crystals

Azulay,

Fraenkel,

Chai

2023

Crystal Growth & Design

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“…The calcite nucleus was formed stably in advance under the control of ECM, which aggregated into a calcite crystal form. Azulay and Chai [32] pointed out that biomineralization can significantly change morphology and structure under the guidance of organic molecules and studied the influence of biopolymer additives on the crystal structure of calcium carbonate. Without additives, carbonate crystals mainly exist in the form of calcite, which is the most thermodynamically stable.…”

Section: Introductionmentioning

confidence: 99%

Experimental Study on the Effect of an Organic Matrix on Improving the Strength of Tailings Strengthened by MICP

Hu,

Zheng,

et al. 2023

Materials

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In order to improve the effect of microbial-induced calcium carbonate precipitation (MICP) in tailings reinforcement, sodium citrate, an organic matrix with good water solubility, was selected as the crystal form adjustment template for inducing calcium carbonate crystallization, and the reinforcements of tailings by MICP were conducted in several experiments. The effects of sodium citrate on the yield, crystal form, crystal appearance, and distribution of calcium carbonate were analyzed by MICP solution test; thus, the related results were obtained. These showed that the addition of a proper amount of organic matrix sodium citrate could result in an increment in the yield of calcium carbonate. The growth rate of calcium carbonate reached 22.6% under the optimum amount of sodium citrate, and the crystals of calcium carbonate were diverse and closely arranged. Based on this, the MICP reinforcement test of tailings was carried out under the action of the optimum amount of sodium citrate. The microscopic analysis using CT and other means showed that the calcium carbonate is distributed more uniformly in tailings, and the porosity of samples is significantly reduced by layered scanning analysis. The results of triaxial shear tests showed that adding organic matrix sodium citrate effectively increased the cohesion, internal friction angle, and peak stress of the reinforced tailings. It aims to provide a novel idea, a creative approach, and a method to enhance the reinforcement effect of tailings and green solidification technology in the mining environment.

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Bacterial epigenetics and its implication for agriculture, probiotics development, and biotechnology design

Povolotsky,

Levy Barazany,

Shacham

et al. 2024

Biotechnology Advances

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Calcium Carbonate Formation in the Presence of Biopolymeric Additives

Cited by 3 publications

References 0 publications

A Bacterial Biofilm Polysaccharide Affects the Morphology and Structure of Calcium Oxalate Crystals

A Bacterial Biofilm Polysaccharide Affects the Morphology and Structure of Calcium Oxalate Crystals

Experimental Study on the Effect of an Organic Matrix on Improving the Strength of Tailings Strengthened by MICP

Bacterial epigenetics and its implication for agriculture, probiotics development, and biotechnology design

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