Calcium biosensing with a sol–gel immobilized photoprotein

Blyth, David J.; Poynter, Sarah J.; Russell, David A.

doi:10.1039/an9962101975

Cited by 31 publications

(14 citation statements)

References 15 publications

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“…This increase in porosity with an increase in R value is similar to what other research has shown. Blyth et al [30] believed that a high water content can increase the pore size in a two-step acid-base catalyzed sol-gel glass. Kortesuo et al [31] reported that an increase in the R value from 6 to 28 increased both the release rate and the total amount of a drug released from acid-catalyzed, tetraethyl orthosilicate (TEOS) sol-gel glass.…”

Section: Discussionmentioning

confidence: 99%

Iron specificity of a biosensor based on fluorescent pyoverdin immobilized in sol-gel glass

Yoder

Kisaalita

2011

J Biol Eng

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Two current technologies used in biosensor development are very promising: 1. The sol-gel process of making microporous glass at room temperature, and 2. Using a fluorescent compound that undergoes fluorescence quenching in response to a specific analyte. These technologies have been combined to produce an iron biosensor. To optimize the iron (II or III) specificity of an iron biosensor, pyoverdin (a fluorescent siderophore produced by Pseudomonas spp.) was immobilized in 3 formulations of porous sol-gel glass. The formulations, A, B, and C, varied in the amount of water added, resulting in respective R values (molar ratio of water:silicon) of 5.6, 8.2, and 10.8. Pyoverdin-doped sol-gel pellets were placed in a flow cell in a fluorometer and the fluorescence quenching was measured as pellets were exposed to 0.28 - 0.56 mM iron (II or III). After 10 minutes of exposure to iron, ferrous ion caused a small fluorescence quenching (89 - 97% of the initial fluorescence, over the range of iron tested) while ferric ion caused much greater quenching (65 - 88%). The most specific and linear response was observed for pyoverdin immobilized in sol-gel C. In contrast, a solution of pyoverdin (3.0 μM) exposed to iron (II or III) for 10 minutes showed an increase in fluorescence (101 - 114%) at low ferrous concentrations (0.45 - 2.18 μM) while exposure to all ferric ion concentrations (0.45 - 3.03 μM) caused quenching. In summary, the iron specificity of pyoverdin was improved by immobilizing it in sol-gel glass C.

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Section: Discussionmentioning

confidence: 99%

Iron specificity of a biosensor based on fluorescent pyoverdin immobilized in sol-gel glass

Yoder

Kisaalita

2011

J Biol Eng

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“…Immobilization did not affect protein's calcium ion‐binding capacity. An optical biosensor for detection of calcium ion was successfully built 128 . Sensors based on entrapped bacteria/cell have also been realized.…”

Section: Applications Of Biodoped Ceramicsmentioning

confidence: 99%

Biodoped Ceramics: Synthesis, Properties, and Applications

Gadre¹,

Gouma²

2006

Journal of the American Ceramic Society

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This feature article focuses on biodoped ceramics. These are inorganic materials in which biological materials are incorporated, thus adding new functionality to them. A brief overview of the prominent synthesis techniques for biodoped ceramics, with emphasis on modified sol-gel processes for metal oxide matrices, is given first. Theoretical treatments of the encapsulation of biologicals within a porous ceramic matrix are reviewed. Experimental studies of the stability and dynamics of protein entrapment in silica and other ceramic matrices are also discussed. Finally, key applications of biodoped ceramics in biochemical species detection, bio-catalysis, and drug delivery are presented.

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“…Oxides obtained via the sol-gel method can be applied in an assortment of ways, both in medicine and pharmaceutical business, as biocatalysts, biosensors, immunodiagnostics, and drug delivery systems (Dave et al 1994;Wu, Lin, and Chan 1994;Blyth, Poynter, and Russel 1996;Sieminska and Zerda 1996;Ahola et al 2000;Li et al 2002;Nguyen et al 2002;Radin et al 2002;Czarnobaj and Lukasiak 2004;de Gaetano et al 2005;Czarnobaj, Prokopowicz, and Lukasiak 2006;Czarnobaj and Lukasiak (2007). Sol-gel derived silica xerogels are nontoxic and biocompatible in vivo.…”

Section: Introductionmentioning

confidence: 99%

Preparation and Characterization of Silica Xerogels as Carriers for Drugs

Czarnobaj

2008

Drug Delivery

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The aim of the present study was to utilize the sol-gel method to synthesize different forms of xerogel matrices for drugs and to investigate how the synthesis conditions and solubility of drugs influence the change of the profile of drug release and the structure of the matrices. Silica xerogels doped with drugs were prepared by the sol-gel method from a hydrolyzed tetraethoxysilane (TEOS) solution containing two model compounds: diclofenac diethylamine, (DD)-a water-soluble drug or ibuprofen, (IB)-a water insoluble drug. Two procedures were used for the synthesis of sol-gel derived materials: one-step procedure (the sol-gel reaction was carried out under acidic or basic conditions) and the two-step procedure (first, hydrolysis of TEOS was carried out under acidic conditions, and then condensation of silanol groups was carried out under basic conditions) in order to obtain samples with altered microstructures. In vitro release studies of drugs revealed a similar release profile in two steps: an initial diffusion-controlled release followed by a slower release rate. In all the cases studied, the released amount of DD was higher and the released time was shorter compared with IB for the same type of matrices. The released amount of drugs from two-step prepared xerogels was always lower than that from one-step base-catalyzed xerogels. One-step acid-catalyzed xerogels proved unsuitable as the carriers for the examined drugs.

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Calcium biosensing with a sol–gel immobilized photoprotein

Cited by 31 publications

References 15 publications

Iron specificity of a biosensor based on fluorescent pyoverdin immobilized in sol-gel glass

Iron specificity of a biosensor based on fluorescent pyoverdin immobilized in sol-gel glass

Biodoped Ceramics: Synthesis, Properties, and Applications

Preparation and Characterization of Silica Xerogels as Carriers for Drugs

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