Calcium-Binding Proteins in Serum of Chickens: Vitellogenin and Albumin

Guyer, Robert B.; Grunder, A. A.; Buss, E. G.; Clagett, C. O.

doi:10.3382/ps.0590874

Cited by 20 publications

(3 citation statements)

References 21 publications

(22 reference statements)

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“…Zinc accumulates during the stages when vitellogen is taken up by the oocyte. Since this protein binds calcium, iron, zinc, and copper (Guyer et al, 1980;Schjeide, 1985;Richards, 19891, processes involving this molecule also have to be considered.…”

Section: Discussionmentioning

confidence: 99%

Zinc, iron, and copper contents of Xenopus laevis oocytes and embryos

Nomizu

Falchuk

Vallée

1993

Molecular Reproduction Devel

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Zinc is essential for vertebrate development; its deficiency results in multiple congenital malformations. Knowledge of the zinc biochemistry that underlies embryologic development is very limited. This has led us to investigate the zinc, iron, and copper contents of Xenopus laevis oocytes and embryos. Stage 1-6 oocytes, isolated from ovaries, and stage 1-40 embryos, obtained by in vitro fertilization techniques, were washed in metal-free water prior to digestion by 70% ultrapure HNO3. The metal content of the digests was analyzed by atomic absorption spectrometry. Stage 6 oocytes contain 65.8 +/- 4, 31.1 +/- 3, and 0.68 +/- 0.2 ng of zinc, iron and copper, respectively. The corresponding concentrations are 1, 0.5, and 0.01 mM in 1 microliter eggs. The metal content varies as a function of egg maturation. The zinc content increases from 3-7 to> 60 ng by stages 3 and 6, respectively. A similar pattern is noted for iron, which increases from 2-5 to 30 ng at analogous stages. In contrast, the copper content remains virtually unchanged in oocytes undergoing maturation. Importantly, the total of all three metals does not vary throughout the first 50 stages of development, when all tadpole organs are forming. Hence, the full complement of zinc, iron, and copper needed for incorporation into apoproteins during development is already present at a time when oocyte maturation is completed. The specific metalloproteins that store, donate, and accept these metals during induction and organogenesis and the alterations caused by metal deficiency can now be identified.

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Section: Discussionmentioning

confidence: 99%

Zinc, iron, and copper contents of Xenopus laevis oocytes and embryos

Nomizu

Falchuk

Vallée

1993

Molecular Reproduction Devel

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“…Egg formation begins with ovulation and approximately 8 days later the ovum falls into the infindibulum in the form of a follicle, then finds it´s way to the uterus for calcification which takes 19 hours in the uterus. Ca comes from the intestine and is transfered to the egg shell region by blood and calcium binding protein (CaBP), which facilitates the transfer of Ca in the intestinal wall and uterus (Hudson et al 1971;Gilbert, 1971;Guyer et al 1980). It is known that more than 20 proteins are found in serum and each one has a different function in the body.…”

Section: Biochemical Parameters and Histopathological Findings In Thementioning

confidence: 99%

Biochemical Parameters and Histopathological Findings in the Forced Molt Laying Hens

Mert

Yildirim

2016

Rev. Bras. Cienc. Avic.

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The aim of this study was to evaluate the effects of forced molting using biochemical parameters and histopathological findings in laying hens. 36 Hyline W36 strain laying hens, 90 weeks old were chosen for this research. Eight of these chickens were randomly selected and placed in a cage as the control group before the molting program began. All the others 28 chickens were used for the forced molting program. Eight laying hens were slaughtered at the end of the molting program named as molting group. The remaining 20 hens were fed for 37 days, weighted and slaughtered when they reached the maximum egg production (80%) as postmolting group. Then, blood was analyzed for malondialdehyde, glutathione, catalase, glucose, calcium, phosphorus, albumin, globulin, total protein, triiodothyronine, thyroxine and Vitamin C. The malondialdehyde and glutathione levels of the thyroid and liver tissues were also analyzed along with an examination of the histopathological changes of the liver, ovarium and thyroid glands; and live body, liver, ovarium, thyroid weights and thyroid lengths. In conclusion, it was found that forced molting produces stress and notable side effects in hens, like the oxidant and antioxidant status of the organs, tissue weights and sizes, hormon profiles, blood biochemical and histopathological parameter changes. The activities of thyroid malondialdehyde (p<0.05), liver glutathione (p<0.01), plasma catalase (p<0.001) were significantly decreased in molting group compared to control values, while liver malondialdehyde levels were significantly increased (p<0.001) and thyroid glutathione levels had nonsignificant effect. These levels in molting hens were the first study for veterinary science.

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“…However, details of the mechanism of divalent cation-dependent cell adhesion to a yolk-coated surface is unknown. One possibility is that dissociated gastrula cells adhere electrostatically to the yolk-coated surface through divalent cations which bind to the yolk-granule proteins, since these latter proteins have the ability to bind divalent cations (20)(21)(22) and the surfaces of dissociated gastrula cells have a high negative charge (23,24).…”

Section: Discussionmentioning

confidence: 99%

A Yolk‐granule Component Acts as an Adhesive Material for Dissociated Gastrula Cells of the Newt, Cynops pyrrhogaster

Komazaki

1987

Dev Growth Differ

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Yolk granules were collected from full-grown ovarian oocytes of the newt, Cynops pyrrhogaster, and dissolved in 3% acetic acid or 8 M urea solution. Culture dishes were then coated with either of the yolk-granule solutions. The yolk-coated surfaces acted as adhesive substrata for dissociated gastrula cells, which showed active locomotion when spread on the surfaces. Divalent cation was required for cell adhesion and spreading on the yolk-coated surface. Trypsin and glycosidase digestions of dissociated cells or the yolk-coated surfaces inhibited cell adhesion and spreading. Addition of concanavalin A to the culture medium inhibited cell spreading on the yolk-coated surfaces, while high concentration (50 mM) of the saccharides, D-galactose, D-lactose and D-mannose, had a slightly inhibitory effect on cell spreading.Two yolk components (30-kD and 108-kD proteins) were isolated from yolk granules and applied to the culture dish surfaces. Surfaces coated with the 30-kD protein showed strong adhesiveness for dissociated gastrula cells.A large amount of extracellular matrix (ECM) has been shown to exist in the blastocoelic cavity of the newt gastrula using the freeze-drying method (1). The amount of this blastocoelic ECM is increased notably in the blastula and early gastrula stages (2). A previous study has revealed that the blastocoelic ECM contains proteins of yolk granules as its major components and serves as an adhesive material for dissociated gastrula cells (3). Moreover, it was shown that a component of yolk granules acted as an adhesive material for dissociated gastrula cells when yolk granules were dissolved in 8 M urea solution and applied to culture dish surfaces.Yolk granules are thus considered to be a reservoir which is consumed during early development as a source of energy and building materials. In addition, close correlations between yolk-granule utilization and organizer formation (4) and cyto-differentiation during organogenesis ( 5 , 6 ) have been suggested. However, it was not until the previous study (3) that the nature of this yolk-granule component as an adhesive material was demonstrated.In the present study, further examinations were performed to establish the nature of the yolk-granule component as an adhesive material for dissociated gastrula cells. The results revealed (a) the behaviour of cells on surfaces coated with the yolk-granule component, (b) the influences of divalent cations, enzyme digestion, concanavalin A and saccharides on cell spreading to the yolk-coated surface and (c) the adhesiveness of isolated yolk-granule proteins for cells. 517

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Calcium-Binding Proteins in Serum of Chickens: Vitellogenin and Albumin

Cited by 20 publications

References 21 publications

Zinc, iron, and copper contents of Xenopus laevis oocytes and embryos

Zinc, iron, and copper contents of Xenopus laevis oocytes and embryos

Biochemical Parameters and Histopathological Findings in the Forced Molt Laying Hens

A Yolk‐granule Component Acts as an Adhesive Material for Dissociated Gastrula Cells of the Newt, Cynops pyrrhogaster

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