Calcium-binding protein distribution in the rat brain

Immunohistochemical Localization of Calbindin-D28K (CABP 28K) in the Projections of Teleost Fish Electromotoneurons: A Comparison of Its Distribution with Endogenous Calcium.

“…The quantification of CaBP 28K in the electric organ of Gnathonemus petersii confirms our immunohistochemical observations. The average protein concentration obtained in the present study is in the range of that shown for the kidney [29] or the cerebellum of mammals [3,29].…”

“…Each molecule of CaBP 28K binds four calcium ions [7]. CaBP 28K, first isolated from the chick duodenal mucosa [32] is apparent in several neuronal cell types of the mammalian central nervous system such as the pyramidal cells of the hippocampal area CA1 [3,16,24,25], granule cells of the dentate gyrus [3], and Purkinje cells of the cerebellum [3,18,26]. Its distribution in the rat spinal cord is controversial; Garcia-Segura et al [14] reported on immunolabelling in some neurons of the rat gelatinous substance of Rolando where CaBP 28K was not found by Jande et al [16].…”

Control of calbindin‐D28K expression in developing mouse kidney

“…Expressed in a variety of organs including kidney, intestine, bone, brain, pancreas, and placenta, its precise function remains the subject of speculation (Christakos et al, 1989). In neural tissues, it may act simply as a calcium buffer (Baimbridge et al, 1982). In intestine and kidney, rises in its cytoplasmic concentration correlate with increased calcium flux (Norman, 1990), suggesting either a direct involvement of' calbindin in ion transport or an indirect effect arising from its ability to regulate ion pumps, e.g., Ca, Mg-ATPase (Morgan et al, 1986).…”

Calcium-Binding Proteins in the Central Nervous System.

“…The estimated intracellular concentration of PV, CB and CR is high enough to bear buffering action in the positive cells (6,58,79,106). 5100 j3 has a molecular weight of 10.5 kDa and one functional EF-hand domain.…”