Calcium-Binding Property of Dephosphorylated Caseins

Immobilization of Acid Phosphatase and Its Use for Dephosphorylation of Casein

“…22 ) They draw the conclusion by adding phosphatase inhibitor (sodium fluoride) to suppress the disintegration of the casein micelles by the action of acid milk phosphatase. This was also supported by Yamauchi et al 20 ) that dephosphorylated whole casein sedimented less than native whole casein at pH 7 in the presence of 15mM CaCI 2 . From these results, the phosphoryl group is required for keeping an adequate micelle size in milk.…”

Modifications of structures and functions of caseins: a scientific and technological challenge

“…As other consequences of this dephosphorylation, the charge distribution along the protein chain was also altered affecting the auto-association of caseins. Besides, the calcium binding and precipitability in the presence of calcium were reduced (Pepper and Thompson 1963;Yamauchi et al 1967;Bingham 1976;Molina et al 2007;McCarthy et al 2013). The loss of phosphate groups reduced the number of calcium bridges (Aoki et al 1985).…”

“…The loss of phosphate groups reduced the number of calcium bridges (Aoki et al 1985). Formation of artificial micelles with dephosphorylated caseins was difficult because of the removal of the majority of calcium-binding sites (Pepper and Thompson 1963;Yamauchi et al 1967;Bingham et al 1972;Pearse et al 1986;McCarthy et al 2013) and resulted in the formation of abnormal micelles less stable in presence of calcium and smaller in size (Pepper and Thompson 1963;Knoop and Peters 1975;Pearse et al 1986;Hiller and Lorenzen 2009).…”

Effect of Dephosphorylation of Casein on Its Coagulation and Proteolysis by Chymosin

“…[1][2][3][4][5][6][7] The authors 2 ) have reported about the effect of enzymatic dephosphorylation on the interaction of calcium ions with whole and as ,-caseins. The binding of calcium ions to the phosphate groups of (lsI-casein did not seem essential for precipitation.…”