Calcium-binding casein phosphopeptides-loaded chitosan oligosaccharides core-shell microparticles for controlled calcium delivery: Fabrication, characterization, and in vivo release studies

Zhu, Biyang; Hou, Tao; He, Hui

doi:10.1016/j.ijbiomac.2019.11.014

Cited by 19 publications

(2 citation statements)

References 46 publications

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“…Схожие данные были получены у микрочастиц, нагруженных кальций-связываемыми фосфопептидами казеина. Исследования высвобождения показали устойчивое контролируемое высвобождение кальция и его поглощение [37].…”

Section: применение хитозана для адресной доставки лпunclassified

Applications of chitosan as a polymer carrier for increasing the drugs’ bioavailability

Ustinova¹,

Венгерович

Glinko³

2022

Pharmacy Formulas

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Oral drug delivery is a dynamic research area, yet associated with multiple issues in its using: enzymatic degradation, hydrolysis, low permeability of intestinal epithelium. The review presents a research papers analysis on the development of targeted drug delivery using a biodegradable polymer chitosan. Chitosan application, singly or in composites, is suitable for various drug delivery systems. Upon oral delivery, chitosan serves as a mucoadhesive polymer with controlled and targeted release. During the last five years, various approaches to the delivery of insulin and other drugs had been reported in literature. The main technological strategy for insulin delivery was its protection against intestinal pH, as well as increasing of permeability via transcellular and/or paracellular pathways. It is observed that application of the biopolymer and its derivatives has a controlled absorption profile. In publications on the drugs delivery, most of the research is focused on development and modification of methods for their producing. According to the presented experimental data, there were obtained particles with well-defined spherical shapes and microparticles capacity of 85-97%. A number of research articles provide data on the chitosan application as a mucoadhesive coating for various nano- or microparticles. It was also noted that application of chitosan microparticles contributed to diminishing adverse side effects.

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Section: применение хитозана для адресной доставки лпunclassified

Applications of chitosan as a polymer carrier for increasing the drugs’ bioavailability

Ustinova¹,

Венгерович

Glinko³

2022

Pharmacy Formulas

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“…[ 11 ] For example, caseinophosphopeptides (CPPs) were the first and the most extensively studied calcium‐binding peptides from the casein protein from milk. [ 12,13 ]…”

Section: Introductionmentioning

confidence: 99%

KFP‐1, a Novel Calcium‐Binding Peptide Isolated from Kefir, Promotes Calcium Influx Through TRPV6 Channels

Chang

Chen

et al. 2021

Molecular Nutrition Food Res

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Introduction Kefir is an acidic and alcoholic fermented milk product with multiple health‐promoting benefits. A previous study demonstrated that kefir enhanced calcium absorption in intestinal Caco‐2 cells. In this study, kefir‐fermented peptide‐1 (KFP‐1) is isolated from the kefir peptide fraction, and its function as a calcium‐binding peptide is characterized. Methods and Results KFP‐1 was identified as a 17‐residue peptide with a sequence identical to that of κ‐casein (residues 138–154) in milk protein. KFP‐1 is demonstrated to promote calcium influx in Caco‐2 and IEC‐6 small intestinal cells in a concentration‐dependent manner. TRPV6, but not L‐type voltage‐gated calcium channels, is associated with the calcium influx induced by KFP‐1. An in vitro calcium binding assay indicates that the full‐length KFP‐1 peptide has a higher calcium‐binding capacity than the two truncated KFP‐1 peptides, KFP‐1∆C5 and KFP‐1C5. Alexa Fluor 594 labeling shows that KFP‐1 is taken up by Caco‐2 cells and interacts with calcium ions and TRPV6 protein. Moreover, KFP‐1 is found moderately resistant to pepsin and pancreatin digestions and enhanced calcium uptake by intestinal enterocytes in vivo. Conclusion These data suggest that KFP‐1, a novel calcium‐binding peptide, binds extracellular calcium ions and enters Caco‐2 and IEC‐6 cells, and promotes calcium uptake through TRPV6 calcium channels. The present study is of great importance for developing kefir‐derived metal ion‐binding peptides as functional nutraceutical additives.

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Response surface methodology and optimization of hydrolysis conditions for the in vitro calcium-chelating and hypoglycemic activities of casein protein hydrolysates prepared using microbial proteases

Megrous,

Zhao,

Al-Dalali

et al. 2024

Food Measure

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Calcium-binding casein phosphopeptides-loaded chitosan oligosaccharides core-shell microparticles for controlled calcium delivery: Fabrication, characterization, and in vivo release studies

Cited by 19 publications

References 46 publications

Applications of chitosan as a polymer carrier for increasing the drugs’ bioavailability

Applications of chitosan as a polymer carrier for increasing the drugs’ bioavailability

KFP‐1, a Novel Calcium‐Binding Peptide Isolated from Kefir, Promotes Calcium Influx Through TRPV6 Channels

Response surface methodology and optimization of hydrolysis conditions for the in vitro calcium-chelating and hypoglycemic activities of casein protein hydrolysates prepared using microbial proteases

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