Calcium balance in sea bream (Sparus aurata): the effect of oestradiol-17beta

Guerreiro, Pedro M.; Fuentes, Juan; Canário, Adelino V.M.; Power, Deborah M.

doi:10.1677/joe.0.1730377

Cited by 90 publications

(69 citation statements)

References 35 publications

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“…As described above, E2 -treatment increases whole body calcium accumulation and stimulates reabsorption of calcium from dermal scales (Guerreiro et al, 2002). As pthrp and pth lr are expressed in hepatocytes, a para-/autocrine role for PTHrP in vitellogenesis was indicated, in analogy to the situation in mam mals (Cros et al, 1998).…”

Section: Vitellogenesismentioning

confidence: 94%

Parathyroid hormone-related protein in teleost fish

Abbink

Flik

2007

General and Comparative Endocrinology

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A brief description is given of the discovery of PTHrP and the roles of the peptide in mammalian physiology. Next, the occurrence of PTHrP in the earliest vertebrates, sharks, skates and fishes, is reviewed and the calciotropic functions of PTHrP are addressed more spe cifically in fishes. Parathyroid hormone-related protein (PTHrP) is a hypercalcemic hormone in teleostean fishes, but also has para-and autocrine functions. After the isolation and identification of fish PTHrP and PTHrP receptors and the subsequent development of recom binant protein and a real-time quantitative PCR, a calciotropic role of PTHrP in fish physiology could be assessed. PTHrP influences calcium physiology via regulation of calcium mobilisation from internal sources (bone and scales) and via calcium uptake from the envi ronment (water and diet). Continuous variations in the need for calcium and in the availability of environmental calcium require fast calciotropes to guarantee calcium balance, in which PTHrP is pivotal for the fish. PTHrP is essential in fish bone physiology, e.g. in min eralisation and calcium reabsorption from the scales. Moreover, PTHrP plays a role in vitellogenesis, cortisol production, regulation of renal Mrp2 activity and melatonin synthesis. The plethora of functions of PTHrP in fish concern endocrine, paracrine and autocrine (and possibly intracrine) functions; calciotropic actions of PTHrP at the organismal and cellular level are prominent in fish. The strong con servation of the pthrp gene in the vertebrate lineage and the N-terminal similarity of the coded proteins relates to the important role of PTHrP in calcium physiology that is of paramount importance to all physiological processes. Recent and ongoing studies will contribute to our rapidly expanding knowledge of the original physiological functions of PTHrP in teleost fish.

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Section: Vitellogenesismentioning

confidence: 94%

Parathyroid hormone-related protein in teleost fish

Abbink

Flik

2007

General and Comparative Endocrinology

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“…Extracts were dried under nitrogen, reconstituted in 0.5 M phosphate-gelatine buffer, pH 7.6 and steroids measured by radioimmunoassay (RIA): E 2 (Guerreiro et al, 2002) and 17,20bP (Canario et al, 1989) and testosterone. The testosterone antiserum was kindly donated by Dr. A.P.…”

Section: Steroid Analysismentioning

confidence: 99%

Tilapia male urinary pheromone stimulates female reproductive axis

Huertas

Almeida

Canário

et al. 2014

General and Comparative Endocrinology

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a b s t r a c tMozambique tilapia males congregate in leks where they establish dominance hierarchies and attract females to spawn in sandy pits. Dominant males store more urine than subordinates and the pattern of urination and the high sensitivity of females to male urine suggest chemical signalling via the urine. Here we show that pre-ovulated and post-spawn females when exposed to dominant male urine increased significantly, in less than 1 h, the release rate of the maturation-inducing steroid 17,20b-dihydroxypregn-4-en-3-one which is maintained elevated for at least 6 h. This indicates a pheromonal role for male urine in the synchronisation of spawning. Furthermore, we show that the lack of affinity of 17,20bP to sex steroid binding globulin explains, at least partly, its rapid release and lack of detection in the blood. Thus tilapia urine involvement in several communication processes confirms that cichlids have evolved a sophisticated chemical signalling system together with their complex visual, acoustic and behavioural displays.

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“…* and ** indicate statistically significant differences at P < 0.05 and P < 0.01, respectively, from the values of the control scales (n = 8 samples; one sample from one fish). estrogen in the scale osteoclasts of freshwater teleosts was higher than that in those of marine teleosts (Persson et al, 1994;Persson et al, 1995;Guerreiro et al, 2002). We believe that osteoclasts in freshwater teleosts have some important roles in calcium metabolism as compared with those in marine teleosts.…”

Section: Discussionmentioning

confidence: 67%

Effects of Inorganic Mercury and Methylmercury on Osteoclasts and Osteoblasts in the Scales of the Marine Teleost as a Model System of Bone

et al. 2014

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To evaluate the effects of inorganic mercury (InHg) and methylmercury (MeHg) on bone metabolism in a marine teleost, the activity of tartrate-resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP) as indicators of such activity in osteoclasts and osteoblasts, respectively, were examined in scales of nibbler fish (Girella punctata). We found several lines of scales with nearly the same TRAP and ALP activity levels. Using these scales, we evaluated the influence of InHg and MeHg. TRAP activity in the scales treated with InHg (10 -5 and 10 -4 M) and MeHg (10 -6 to 10 -4 M) during 6 hrs of incubation decreased significantly. In contrast, ALP activity decreased after exposure to InHg (10 -5 and 10 -4 M) and MeHg (10 -6 to 10 -4 M) for 18 and 36 hrs, although its activity did not change after 6 hrs of incubation. As in enzyme activity 6 hrs after incubation, mRNA expression of TRAP (osteoclastic marker) decreased significantly with InHg and MeHg treatment, while that of collagen (osteoblastic marker) did not change significantly. At 6 hrs after incubation, the mRNA expression of metallothionein, which is a metal-binding protein in osteoblasts, was significantly increased following treatment with InHg or MeHg, suggesting that it may be involved in the protection of osteoblasts against mercury exposure up to 6 hrs after incubation. To our knowledge, this is the first report of the effects of mercury on osteoclasts and osteoblasts using marine teleost scale as a model system of bone.

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Calcium balance in sea bream (Sparus aurata): the effect of oestradiol-17beta

Cited by 90 publications

References 35 publications

Parathyroid hormone-related protein in teleost fish

Parathyroid hormone-related protein in teleost fish

Tilapia male urinary pheromone stimulates female reproductive axis

Effects of Inorganic Mercury and Methylmercury on Osteoclasts and Osteoblasts in the Scales of the Marine Teleost as a Model System of Bone

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