Calcium and vitamin D3 combinations improve fatty liver disease through AMPK-independent mechanisms

Shojaei‐Zarghani, Sara; Soraya, Hamid; Alizadeh, Mohammad

doi:10.1007/s00394-016-1360-4

Cited by 10 publications

(12 citation statements)

References 65 publications

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“…Thus, the inverse association might partly be explained by increased whole-body fat oxidation rates. Fourth, an animal study found that calcium and vitamin D combinations could prevent NAFLD development [37]. Finally, yogurt is seen as a nutrient-dense food linked with healthy diet habitats.…”

Section: Discussionmentioning

confidence: 99%

Association between habitual yogurt consumption and newly diagnosed non-alcoholic fatty liver disease

Zhang

et al. 2019

Eur J Clin Nutr

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Background/Objectives Many studies have suggested that probiotics may be applied as a therapeutic agent for non-alcoholic fatty liver disease (NAFLD). However, the effects of frequent yogurt consumption (as a natural probiotic source) on NAFLD remain poorly understood. This study was to examine the association of habitual yogurt consumption with newly diagnosed NAFLD in the general adult population. Subject/Methods Overall, 24,389 adults were included in this cross-sectional study. Yogurt consumption was estimated by using a validated self-administered food frequency questionnaire. NAFLD was diagnosed by abdominal ultrasonography. We used logistic regression models to assess the association between yogurt consumption categories and newly diagnosed NAFLD. Results The multivariable odds ratios with 95% confidence interval of newly diagnosed NAFLD were 1.00 (0.88, 1.14) for 1 time/week, 0.91 (0.81, 1.02) for 2-3 times/week, and 0.86 (0.76, 0.98) for ≥4 times/week (P for trend = 0.01), compared with those who consumed <1 time/week yogurt. The inverse association was observed in a sensitivity analysis. Conclusion Higher yogurt consumption was inversely associated with the prevalence of newly diagnosed NAFLD. These results are needed to be confirmed in randomized controlled trials or prospective studies.

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Section: Discussionmentioning

confidence: 99%

Association between habitual yogurt consumption and newly diagnosed non-alcoholic fatty liver disease

Zhang

et al. 2019

Eur J Clin Nutr

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“…The calcium content in American Institute of Nutrition (AIN93) standard mice feed is 0.5%. After referring to other groups and our previous research, we chose 1.2% calcium in our calcium supplementary feed [16,17]. Calcium carbonate was used to regulate calcium content in the calcium supplementation group.…”

Section: Mice and Sample Collectionmentioning

confidence: 99%

Dietary calcium supplementation promotes the accumulation of intramuscular fat

Zhang

Pan

Sun

et al. 2021

J Animal Sci Biotechnol

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Background In the livestock industry, intramuscular fat content is a key factor affecting meat quality. Many studies have shown that dietary calcium supplementation is closely related to lipid metabolism. However, few studies have examined the relationship between dietary calcium supplementation and intramuscular fat accumulation. Methods Here, we used C2C12 cells, C57BL/6 mice (n = 8) and three-way cross-breeding pigs (Duroc×Landrace×Large white) (n = 10) to study the effect of calcium addition on intramuscular fat accumulation. In vitro, we used calcium chloride to adjust the calcium levels in the medium (2 mmol/L or 3 mmol/L). Then we measured various indicators. In vivo, calcium carbonate was used to regulate calcium levels in feeds (Mice: 0.5% calcium or 1.2% calcium) (Pigs: 0.9% calcium or 1.5% calcium). Then we tested the mice gastrocnemius muscle triglyceride content, pig longissimus dorsi muscle meat quality and lipidomics. Results In vitro, calcium addition (3 mmol/L) had no significant effect on cell proliferation, but promoted the differentiation of C2C12 cells into slow-twitch fibers. Calcium supplementation increased triglyceride accumulation in C2C12 cells. Calcium addition increased the number of mitochondria and also increased the calcium level in the mitochondria and reduced the of key enzymes activity involved in β-oxidation such as acyl-coenzyme A dehydrogenase. Decreasing mitochondrial calcium level can alleviate lipid accumulation induced by calcium addition. In addition, calcium addition also reduced the glycolytic capacity and glycolytic conversion rate of C2C12 cells. In vivo, dietary calcium supplementation (1.2%) promoted the accumulation of triglycerides in the gastrocnemius muscle of mice. Dietary calcium supplementation (1.5%) had no effect on pig weight, but significantly improved the flesh color of the longissimus dorsi muscle, reduced the backfat thickness and increased intramuscular fat content in pigs. Besides, calcium addition had no effect on longissimus dorsi pH, electrical conductivity and shear force. Conclusions These results suggest that calcium addition promotes intramuscular fat accumulation by inhibiting the oxidation of fatty acids. These findings provide a new tool for increasing intramuscular fat content and an economical strategy for improving meat quality.

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“…Previous studies have shown that high Ca and Vitamin D 3 intakes caused lesser adiposity and lipid accumulation, probably via an insulin and AMPK- independent pathway [ 34 ]. However, few studies have reported about the effect of dietary P on the AMPK pathway.…”

Section: Resultsmentioning

confidence: 99%

Regulation of phosphate transport and AMPK signal pathway by lower dietary phosphorus of broilers

et al. 2017

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Lower available P (aP) was used as a base value in nutritional strategies for mitigating P pollution by animal excreta. We hypothesized that the mechanism regulating phosphate transport under low dietary P might be related with the AMPK signal pathway. A total of 144 one-day-old Arbor Acres Plus broilers were randomly allocated to control (HP) or trial (LP) diets, containing 0.45 and 0.23% aP, respectively. Growth performance, blood, intestinal, and renal samples were tested in 21-day-old broilers. Results shown that LP decreased body weight gain and feed intake. Higher serum Ca and fructose, but lower serum P and insulin were detected in LP-fed broilers. NaPi-IIb mRNA expression in intestine and NaPi-IIa mRNA expression in kidney were higher in the LP group. AMP: ATP, p-AMPK: total AMPK, and p-ACC: total ACC ratios in the duodenal mucosa were decreased in the LP group, whereas the p-mTOR: total mTOR ratio increased. These findings suggested that the increase in phosphate transport owing to LP diet might be regulated either directly by higher mTOR activity or indirectly by the suppressive AMPK signal, with corresponding changes in blood insulin and fructose content. A novel viewpoint on the regulatory mechanism underlying phosphate transport under low dietary P conditions was revealed, which might provide theoretical guidelines for reducing P pollution by means of nutritional regulation.

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Calcium and vitamin D3 combinations improve fatty liver disease through AMPK-independent mechanisms

Cited by 10 publications

References 65 publications

Association between habitual yogurt consumption and newly diagnosed non-alcoholic fatty liver disease

Association between habitual yogurt consumption and newly diagnosed non-alcoholic fatty liver disease

Dietary calcium supplementation promotes the accumulation of intramuscular fat

Regulation of phosphate transport and AMPK signal pathway by lower dietary phosphorus of broilers

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