Calcium Activity of Upper Thoracic Dorsal Root Ganglion Neurons in Zucker Diabetic Fatty Rats

Abstract

The aim of the present study was to examine the calcium activity of C8-T5 dorsal root ganglion (DRG) neurons from Zucker diabetic fatty rats. In total, 8 diabetic ZDF fatty animals and 8 age-matched control ZDF lean rats were employed in the study. C8-T5 dorsal root ganglia were isolated bilaterally from 14 to 18 weeks old rats, and a primary culture was prepared. Calcium activity was measured ratiometrically using the fluorescent Ca2+-indicator Fura-2 acetoxymethyl ester. All neurons were stimulated twice with 20 mM K+, followed by stimulation with either 0.3 or 0.5 μM Capsaicin, alone or in combination with algogenic chemicals (bradykinin, serotonin, prostaglandin E2 (all 10−5 M), and adenosine (10−3 M)) at pH 7.4 and 6.0. Neurons from diabetic animals exhibited an overall increased response to stimulation with 20 mM K+ compared to neurons from control. Stimulation with Capsaicin alone caused an augmented response in neurons from diabetic animals compared to control animals. When stimulated with a combination of Capsaicin and algogenic chemicals, no differences between the two groups of neurons were measured, neither at pH 7.4 nor 6.0. In conclusion, diabetes-induced alterations in calcium activity of the DRG neurons were found, potentially indicating altered neuronal responses during myocardial ischemia.

Agomelatine pretreatment prevents development of hyperglycemia and hypoinsulinemia in streptozotocin‐induced diabetes in mice

“…It has been shown that mitochondrial dysfunction in sensory neurons from type 1 diabetic rats is related to impaired rates of respiratory activity [17], and impaired respiratory function is associated with reduced ROS generation in the mitochondrial matrix of cultured DRG neurons isolated from STZ-diabetic rats [16]. Alterations in calcium activity have been found in DRGs from Zucker Diabetic Fatty Rats [54]. Our study and the related previous studies show that the diabetes induces many effects on peripheral neurons in diabetic animal models [55].…”

Calcium Dyshomeostasis in Neuropathy Diabetes

“…Diminished (in spite of the fact that not generally) and diminished upgrade evoked Ca 2+ signals. Ca 2+ -dealing with disarranges have additionally been found in tangible neurons from creatures with diabetes [11,40,41]. ] I rest was not influenced in neurons from the ganglion in the more elevated levels of the spinal line (C3-L3).…”

“…This distinction connects with the lumbar DRG tactile neuron vulnerability and the long axon of diabetic neuropathy. A portion of these distinctions are identified with contrasts in estimation of [Ca 2+ ]I between considers with different models and term of diabetes, maybe likewise because of contrasts in different sub-populaces of neurons from the degree of DRG in the spine [11,40].…”