Calcitonin-stimulated renal Ca2+ reabsorption occurs independently of TRPV5

Hsu, Yu‐Juei; Dimke, Henrik; Hoenderop, Joost G. J.; Bindels, René J. M.

doi:10.1093/ndt/gfp645

Cited by 13 publications

(8 citation statements)

References 32 publications

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“…Microperfusion experiments in rabbits demonstrated that CT stimulates Ca 2+ absorption in distal convoluted tubules where TRPV5 is not expressed [94]. Injection of CT into mice also stimulated renal Ca 2+ reabsorption without affecting TRPV5 expression, and similar effects were observed in TRPV5 − / − mice, suggesting that the CT acts independently of TRPV in renal Ca 2+ reabsorption [46]. …”

Section: Hormonal Control Of Ion Regulationmentioning

confidence: 99%

Zebrafish as an animal model to study ion homeostasis

Hwang

Chou

2013

Pflugers Arch - Eur J Physiol

158

136

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Zebrafish (Danio rerio) possesses several advantages as an experimental organism, including the applicability of molecular tools, ease of in vivo cellular observation and functional analysis, and rapid embryonic development, making it an emerging model for the study of integrative and regulatory physiology and, in particular, the epithelial transport associated with body fluid ionic homeostasis. Zebrafish inhabits a hypotonic freshwater environment, and as such, the gills (or the skin, during embryonic stages) assume the role of the kidney in body fluid ionic homeostasis. Four types of ionocyte expressing distinct sets of transporters have been identified in these organs: H+-ATPase-rich, Na+-K+-ATPase-rich, Na+-Cl− cotransporter-expressing and K+-secreting cells; these ionocytes perform transepithelial H+ secretion/Na+ uptake/NH4+ excretion, Ca2+ uptake, Na+/Cl− uptake, and K+ secretion, respectively. Zebrafish ionocytes are analogous to various renal tubular cells, in terms of ion transporter expression and function. During embryonic development, ionocyte progenitors develop from epidermal stem cells and then differentiate into different types of ionocyte through a positive regulatory loop of Foxi3a/-3b and other transcription factors. Several hormones, including cortisol, vitamin D, stanniocalcin-1, calcitonin, and isotocin, were found to participate in the control pathways of ionic homeostasis by precisely studying the target ion transport pathways, ion transporters, or ionocytes of the hormonal actions. In conclusion, the zebrafish model not only enhances our understanding of body fluid ion homeostasis and hormonal control in fish but also informs studies on mammals and other animal species, thereby providing new insights into related fields.

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Section: Hormonal Control Of Ion Regulationmentioning

confidence: 99%

Zebrafish as an animal model to study ion homeostasis

Hwang

Chou

2013

Pflugers Arch - Eur J Physiol

158

136

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“…Transient high PTH levels have the opposite effect, mediating increased bone formation by an as yet poorly understood pharmacological mechanism [5] . In contrast, calcitonin inhibits the function of bone-resorbing osteoclasts and reduces urinary calcium excretion [6] . The importance of CaSR in calcium homeostasis is emphasized by the pathological conditions of calcium homeostasis caused by inactivating and activating CaSR mutations such as Familial Hypocalciuric Hypercalcaemia (FHH), Neonatal Severe Hyperparathyroidism (NSHPT) [7] , [8] ; and Autosomal Dominant Hypocalcaemia (ADH) [9] .…”

Section: Introductionmentioning

confidence: 99%

Role of Receptor Activity Modifying Protein 1 in Function of the Calcium Sensing Receptor in the Human TT Thyroid Carcinoma Cell Line

et al. 2014

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The Calcium Sensing Receptor (CaSR) plays a role in calcium homeostasis by sensing minute changes in serum Ca2+ and modulating secretion of calciotropic hormones. It has been shown in transfected cells that accessory proteins known as Receptor Activity Modifying Proteins (RAMPs), specifically RAMPs 1 and 3, are required for cell-surface trafficking of the CaSR. These effects have only been demonstrated in transfected cells, so their physiological relevance is unclear. Here we explored CaSR/RAMP interactions in detail, and showed that in thyroid human carcinoma cells, RAMP1 is required for trafficking of the CaSR. Furthermore, we show that normal RAMP1 function is required for intracellular responses to ligands. Specifically, to confirm earlier studies with tagged constructs, and to provide the additional benefit of quantitative stoichiometric analysis, we used fluorescence resonance energy transfer to show equal abilities of RAMP1 and 3 to chaperone CaSR to the cell surface, though RAMP3 interacted more efficiently with the receptor. Furthermore, a higher fraction of RAMP3 than RAMP1 was observed in CaSR-complexes on the cell-surface, suggesting different ratios of RAMPs to CaSR. In order to determine relevance of these findings in an endogenous expression system we assessed the effect of RAMP1 siRNA knock-down in medullary thyroid carcinoma TT cells, (which express RAMP1, but not RAMP3 constitutively) and measured a significant 50% attenuation of signalling in response to CaSR ligands Cinacalcet and neomycin. Blockade of RAMP1 using specific antibodies induced a concentration-dependent reduction in CaSR-mediated signalling in response to Cinacalcet in TT cells, suggesting a novel functional role for RAMP1 in regulation of CaSR signalling in addition to its known role in receptor trafficking. These data provide evidence that RAMPs traffic the CaSR as higher-level oligomers and play a role in CaSR signalling even after cell surface localisation has occurred.

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“…The calcitonin receptor is present in the DCT (34,175), but the molecular mechanism of calcitonin-stimulated calcium reabsorption in the distal nephron is not clear. It does not involve altered TRPV5 or CaBP-D28K (80).…”

Section: Dct and Connecting Tubulementioning

confidence: 86%

Ways of calcium reabsorption in the kidney

Moor

Bonny

2016

American Journal of Physiology-Renal Physiology

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The role of the kidney in calcium homeostasis has been reshaped from a classic view in which the kidney was regulated by systemic calcitropic hormones such as vitamin D3 or parathyroid hormone to an organ actively taking part in the regulation of calcium handling. With the identification of the intrinsic renal calcium-sensing receptor feedback system, the regulation of paracellular calcium transport involving claudins, and new paracrine regulators such as klotho, the kidney has emerged as a crucial modulator not only of calciuria but also of calcium homeostasis. This review summarizes recent molecular and endocrine contributors to renal calcium handling and highlights the tight link between calcium and sodium reabsorption in the kidney.

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Calcitonin-stimulated renal Ca2+ reabsorption occurs independently of TRPV5

Cited by 13 publications

References 32 publications

Zebrafish as an animal model to study ion homeostasis

Zebrafish as an animal model to study ion homeostasis

Role of Receptor Activity Modifying Protein 1 in Function of the Calcium Sensing Receptor in the Human TT Thyroid Carcinoma Cell Line

Ways of calcium reabsorption in the kidney

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