Calcitonin gene-related peptide stimulates proliferation and osteogenic differentiation of osteoporotic rat-derived bone mesenchymal stem cells

Abstract

Osteoporosis, a systemic bone disorder, is prevalent in postmenopausal woman. Bone mesenchymal stem cells (BMSCs), precursors of osteogenic cells, may contribute to prevention or treatment of bone frustrate in osteoporosis. Recently, two studies suggested a role of calcitonin gene-related peptide (CGRP) in promoting osteogenesis of BMSCs under physiological conditions. However, the role of CGRP on BMSCs, which are derived from osteoporotic tissues, is unclear. Here, we investigated the role of CGRP on BMSCs isolated from female osteoporotic rats. Data showed that CGRP stimulated cell proliferation and inhibited cell apoptosis for short-term culture of BMSCs. Instead, CGRP induced BMSCs differentiation into the osteoblasts and promoted formation of calcified nodules after long-term culture. Moreover, CGRP gradually up-regulated expression levels of osteoporotic differentiation-related genes including alkaline phosphatase, Collagen type I, Bmp2, Osteonectin, and Runx2 during osteogenic differentiation. In conclusion, CGRP promoted proliferation and induced osteogenic differentiation and mineralization during female osteoporotic rat-derived BMSC differentiation. These findings support a potential role of CGRP on the prevention or treatment of osteoporotic fracture.

Prevention of Bone Loss in a Model of Postmenopausal Osteoporosis through Adrenomedullin Inhibition

“…But, since we obtained the reverse result (Figure 3 ), we hypothesized that our observations must be due to an indirect systemic effect of AM. A possible mechanism may involve the increase in CGRP shown in Figure 1D , since CGRP can induce bone growth through stimulation of bone mesenchymal stem cells (Liang et al, 2015 ). Also, AM has been shown to reduce insulin secretion in the pancreas, thus increasing glucose levels (Martinez et al, 1996 ).…”

Calcitonin gene‑related peptide reduces Porphyromonas�gingivalis LPS‑induced TNF‑α release and apoptosis in osteoblasts

“…A number of previous reports have focused on the effects of CGRP on cultured osteoblasts and have revealed that CGRP can regulate bone metabolism and stimulate osteoblasts differentiation (17,30,31). Nevertheless, only a few reports have reported on LPS-induced osteoblast apoptosis.…”

Enhanced Proliferation and Differentiation Effects of a CGRP- and Sr-Enriched Calcium Phosphate Cement on Bone Mesenchymal Stem Cells

“…CGRP participates in the regulation of growth, repair and performance Effects of a novel CGRP-Sr-CPC on osteoporotic rat-derived BMSCs e438 of bone by working on BMSCs (40)(41)(42). Our recent study discovered positive effects of CGRP in stimulation of cell growth and induction of osteogenic differentiation in BMSCs derived from osteoporosis (11). Based on the promising effects of CGRP on BMSCs, we created CGRP-CPC.…”