Calcitonin Gene-Related Peptide-Like Immunoreactivity in the Human Small Intestine

Timmermans, Jean‐Pierre; Scheuermann, D.W.; Barbiers, M.; Adriaensen, Dirk; Stach, W; Hee, R. Van; Groodt-Lasseel, M.H.A. de

doi:10.1159/000147227

Cited by 49 publications

(42 citation statements)

References 8 publications

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“…Additionally, immunoreactivity to CGRP was found in endocrine cells in the human small intestine [5,19,21]. In the present study, CGRP was found in tissues of the large intestine obtained from patients suffering from intestinal cancer.…”

Section: Discussionsupporting

confidence: 62%

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Concentration of vasoactive intestinal polypeptide, neuropeptide Y and calcitonin gene related peptide in neoplastic tissue of the human large intestine

Godlewski¹,

Pidsudko²,

Sienkiewicz³

2011

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A Ai im m o of f t th he e s st tu ud dy y: : The purpose of this study was to investigate the presence of vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY) and calcitonin gene related peptide (CGRP) neuropeptides in neoplastic tissue of the large intestine. The results obtained were compared to an unchanged part of the intestine originating from the same patient. M Ma at te er ri ia al l a an nd d m me et th ho od ds s: : The tissue samples were taken from the postoperative part of the intestine, resected during an operation due to treatment of colon cancer. Tissues were taken from 15 patients. Concentrations of neuropeptides were determined by ELISA tests, and results were calculated per 1 g of fresh tissue. The data obtained were statistically analysed. R Re es su ul lt ts s: : In tissues of the neoplastic tumour of the sigmoid colon and rectum the concentration of VIP and NPY was determined (28.9 ng/g and 8.6 ng/g, respectively). The observed values were statistically significantly lower than those observed in corresponding tissues of unchanged intestinal segments (83.9 ng/g for VIP and 19.5 ng/g for NPY). Surprisingly, amounts of CGRP were very similar in both neoplastic and intact tissues (13.3 ng/g and 13.4 ng/g respectively). C Co on nc cl lu us si io on ns s: : Immunoenzymatic investigations allow one to establish the concentration of neuropeptides associated with the enteric nervous system in cancer tissues of the large intestine. Various concentrations of these neuropeptides were observed in particular patients of both compared groups. The performed statistical analysis did not reveal associations between degree of cancer infiltration within the colonic wall and the level of presence of investigated neuropeptides in either cancer or control tissues. The fact of presence of these neuropeptides in cancer tissues confirmed by this study should be underlined at the same time.K Ke ey y w wo or rd ds s: : colon cancer, enteric nervous system, neuropeptides, calcitonin gene related peptide, neuropeptide Y, vasoactive intestinal polypeptide.

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Section: Discussionsupporting

confidence: 62%

“…In physiological conditions, within nervous structures supplying human large intestines the presence of vasoactive intestinal polypeptide (VIP), calcitonin gene related peptide (CGRP) and neuropeptide Y (NPY) was found [1][2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Concentration of vasoactive intestinal polypeptide, neuropeptide Y and calcitonin gene related peptide in neoplastic tissue of the human large intestine

Godlewski¹,

Pidsudko²,

Sienkiewicz³

2011

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“…Immunohistochemical studies have demonstrated the expression of CGRP in neurons and nerve fibres in the submucous and myenteric plexuses in the human small intestine [12] as well as in the small and large intestine of guinea pig and pig [27][28][29][30][31]. Immunohistochemical colocalization investigations have revealed that in the submucous plexuses, CGRP is expressed by local sensory neurons or cholinergic secretomotor neurons.…”

Section: Discussionmentioning

confidence: 99%

“…Investigations performed under physiological conditions have demonstrated the expression of vasoactive intestinal peptide (VIP), neuropeptide Y (NPY), pituitary adenylate cyclase-activating polypeptide (PACAP), somatostatin (SOM), substance P (SP) and calcitonin generelated peptide (CGRP) in nerve elements of the enteric nervous system (ENS) supplying the human large intestine [1,[6][7][8][9][10][11][12][13]. Some studies have also revealed profound morphological and functional changes in the ENS associated with pathological conditions of the colon [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Somatostatin, substance P and calcitonin gene-related peptide-positive intramural nerve structures of the human large intestine affected by carcinoma.

Godlewski

Kaleczyc²

2010

Folia Histochem Cytobiol.

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Abstract:The aim of this study was to investigate the arrangement and chemical coding of enteric nerve structures in the human large intestine affected by cancer. Tissue samples comprising all layers of the intestinal wall were collected during surgery form both morphologically unchanged and pathologically altered segments of the intestine (n=15), and fixed by immersion in buffered paraformaldehyde solution. The cryostat sections were processed for double-labelling immunofluorescence to study the distribution of the intramural nerve structures (visualized with antibodies against protein gene-product 9.5) and their chemical coding using antibodies against somatostatin (SOM), substance P (SP) and calcitonin gene-related peptide (CGRP). The microscopic observations revealed distinct morphological differences in the enteric nerve system structure between the region adjacent to the cancer invaded area and the intact part of the intestine. In general, infiltration of the cancer tissue resulted in the gradual (depending on the grade of invasion) first decomposition and reduction to final partial or complete destruction and absence of the neuronal elements. A comparative analysis of immunohistochemically labeled sections (from the unchanged and pathologically altered areas) revealed a statistically significant decrease in the number of CGRP-positive neurons and nerve fibres in both submucous and myenteric plexuses in the transitional zone between morphologically unchanged and cancer-invaded areas. In this zone, a decrease was also observed in the density of SP-positive nerve fibres in all intramural plexuses. Conversely, the investigations demonstrated statistically insignificant differences in number of SP-and SOM-positive neurons and a similar density of SOM-positive nerve fibres in the plexuses of the intact and pathologically changed areas. The differentiation between the potential adaptive changes in ENS or destruction of its elements by cancer invasion should be a subject of further investigations.

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“…After sympathetic effects are eliminated, MN stimulation causes a contraction that is abolished by capsaicin and is probably due to the release of either substance P or calcitonin gene-related peptide (Barthó & Holzer, 1985;De Luca & Rand, 1990). These nerves do not appear to innervate the muscle layers directly (Gibbins et al 1985;Timmermans et al 1992). Furthermore, contractions of the circular muscle due to capsaicin-induced transmitter release from extrinsic primary afferent nerves are abolished by TTX (Barthó et al 1994).…”

Section: Excitatory Effects Of Mn Stimulationmentioning

confidence: 99%

Role of α₂‐adrenoceptors in the sympathetic inhibition of motility reflexes of guinea‐pig ileum

Stebbing

Johnson

Vremec

et al. 2001

The Journal of Physiology

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1. Sympathetic regulation of the motility of guinea-pig ileum was investigated using mesenteric nerve (MN) stimulation to inhibit motility reflexes, in vitro.2. Transmural electrical stimulation (5 Hz, 1 s) in intact intestinal segments, or inflation of a balloon against the mucosa in opened segments, evoked contractions of the circular and longitudinal muscles oral to the stimulus.3. MN stimulation (10 Hz, 5 s) usually abolished contractions of the longitudinal and circular muscles evoked by either electrical or mechanical stimuli.4. The inhibition was mimicked by UK14,304 (70-100 nM) and abolished by idazoxan (100 nM), revealing an enhancement of circular muscle contractions. There was no evidence for a 2 -receptors on the muscle, suggesting sympathetic inhibition was via the myenteric plexus.5. Possible sites of action of noradrenaline released from sympathetic nerves were investigated using intracellular recordings from the circular muscle in a multichambered organ bath.6. When in the stimulation chamber, UK14,304 depressed (by 50 %) excitatory junction potentials (EJPs) recorded oral to a distension stimulus, but did not affect inhibitory junction potentials (IJPs) recorded anal to the stimulus. When added to a chamber between the stimulus and recording chambers, UK14,304 depressed EJPs by 40 %, but did not alter IJPs. When in the recording chamber, UK14,304 depressed EJPs by 20 %, but had no effect on IJPs. IJPs were inhibited, however, when UK14,304 was applied to the whole bath.7. It is concluded that sympathetic activity inhibits intestinal motility mainly via a 2 -adrenoceptors on ascending interneurons and intrinsic sensory neurons of the orally directed reflex pathway.

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Calcitonin Gene-Related Peptide-Like Immunoreactivity in the Human Small Intestine

Cited by 49 publications

References 8 publications

Concentration of vasoactive intestinal polypeptide, neuropeptide Y and calcitonin gene related peptide in neoplastic tissue of the human large intestine

Concentration of vasoactive intestinal polypeptide, neuropeptide Y and calcitonin gene related peptide in neoplastic tissue of the human large intestine

Somatostatin, substance P and calcitonin gene-related peptide-positive intramural nerve structures of the human large intestine affected by carcinoma.

Role of α₂‐adrenoceptors in the sympathetic inhibition of motility reflexes of guinea‐pig ileum

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Calcitonin Gene-Related Peptide-Like Immunoreactivity in the Human Small Intestine

Cited by 49 publications

References 8 publications

Concentration of vasoactive intestinal polypeptide, neuropeptide Y and calcitonin gene related peptide in neoplastic tissue of the human large intestine

Concentration of vasoactive intestinal polypeptide, neuropeptide Y and calcitonin gene related peptide in neoplastic tissue of the human large intestine

Somatostatin, substance P and calcitonin gene-related peptide-positive intramural nerve structures of the human large intestine affected by carcinoma.

Role of α2‐adrenoceptors in the sympathetic inhibition of motility reflexes of guinea‐pig ileum

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Role of α₂‐adrenoceptors in the sympathetic inhibition of motility reflexes of guinea‐pig ileum