Calcitonin gene-related peptide (CGRP) immunoreactive sensory nerves in the human and guinea pig uvea and cornea

Uusitalo, Hannu; Krootila, Kari; Palkama, Arto

doi:10.1016/0014-4835(89)90030-4

Cited by 48 publications

(21 citation statements)

References 25 publications

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“…The results of the present investigation confirm and extend previous immunohistochemical demonstrations of CGRP-, SP-, and GAL-IR nerve fibers in the mammalian cornea (Stone et al, 1982 ;Tervo et al, 1982a,b ;Ehinger et al, 1983 ;Miller et al, 1983 ;Sasaoka et al, 1984 ;Stone et al, 1986a ;Kuwayama and Stone, 1987 ;Stone et al, 1987 ;Silverman and Kruger, 1988 ;Ueda, LoCascio and Aquavella, 1989 ;Uusitalo, Krootila and Palkama, 1989 ;Jones and Marfurt, 1991 ;Beckers et al, 1992). However, with few exceptions (Sasaoka et al, 1984 ;Silverman and Kruger, 1988 ;Jones and Marfurt, 1991) most investigators examined random corneal sections cut perpendicular to the corneal surface and did not attempt to determine the relative densities or to reconstruct the three-dimensional innervation patterns of these nerves.…”

Section: Corneal Sensory Peptidergic Innervationsupporting

confidence: 92%

“…The results of the selective ocular denervation studies confirm previous reports that corneal CGRPand SP-IR nerve fibers derive exclusively from the trigeminal ganglion (Tervo et al, 1982a ;Miller et al, 1983 ;Sasaoka et al, 1984 ;Stone et al, 1986a ;Kuwayama and Stone, 1987 ;Uusitalo et al, 1989 ;Jones and Marfurt, 1991). The results of this study have shown that most rat GAL-IR corneal nerve fibers are also sensory in origin.…”

Section: Corneal Sensory Peptidergic Innervationsupporting

confidence: 90%

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Peptidergic Innervation of the Rat Cornea

Jones

Marfurt

1998

Experimental Eye Research

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Section: Corneal Sensory Peptidergic Innervationsupporting

confidence: 92%

Section: Corneal Sensory Peptidergic Innervationsupporting

confidence: 90%

Peptidergic Innervation of the Rat Cornea

Jones

Marfurt

1998

Experimental Eye Research

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“…CGRP-IR somata in the rat trigeminal ganglion make up a large proportion of the small-to-medium-sized type B ganglion cells . CGRP-IR was observed mainly in small-diameter cells and was observed less frequently in so-called large cells (diameters of about 40 µm; Lee et al, 1985;Terenghi et al, 1985;Kuwayama et al, 1987;Uusitalo et al, 1989;McCarthy and Lawson, 1990;Alvarez et al, 1991). So-called giant cells (diameters of about 60 µm) did not exhibit immunoreactivity to CGRP, substance P, or cholecystokinin (Kuwayama et al, 1987).…”

Section: Discussionmentioning

confidence: 94%

“…CGRP-IR was detected in the somata of visceral, cutaneous, articular, and muscular afferent units in the dorsal root ganglia (Hoheisel et al, 1994;Yoshida et al, 1995) and in trigeminal ganglion cells innervating the eye of the guinea pig (Kuwayama et al, 1987;Uusitalo et al, 1989). Afferents from the different receptive fields of the ophthalmic nerve, such as EOMs, cornea, and eyelid of the sheep, show CGRP-IR (Bortolami et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

“…Kummer and Heym (1986) reported a greater variability of neuropeptides in the trigeminal ganglion than in other ganglia. Among other neuropeptides, CGRP-like immunoreactivity (CGRP-IR) has been demonstrated in neurons of the trigeminal ganglion (Lee et al, 1985;Gibbins et al, 1987a,b;Stjä rne et al, 1989;Tani et al, 1990;Alvarez et al, 1991;Szabat et al, 1994) and in trigeminal ganglion cells innervating the eye of the guinea pig (Terenghi et al, 1985;Kuwayama et al, 1987;Uusitalo et al, 1989). Trigeminal neurons supplying the EOMs of sheep exhibited CGRP-IR (Bortolami et al, 1991).…”

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confidence: 99%

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Sensory innervation of the guinea pig extraocular muscles: A 1,1?-dioctadecyl-3,3,3?3?-tetramethylindocarbocyanine perchlorate tracing and calcitonin gene-related peptide immunohistochemical study

et al. 1997

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The sensory apparatus of the extraocular muscles attains special interest because of the great variation among different species with respect to the proprioceptors. The sensory innervation of the guinea pig extraocular muscles, lacking both muscle spindles and tendon organs, was investigated with a fluorescence double-labelling method. Primary sensory perikarya were assessed by postmortem application of 1,1'-dioctadecyl-3,3,3'3'-tetramethylindocarbocyanine perchlorate (Di-I) to the extraocular muscle nerves. Traced neurons were found in the ipsilateral ophthalmic part of the trigeminal ganglion. This is in line with findings in other species. Calcitonin gene-related peptide (CGRP) was detected immunohistochemically within the trigeminal ganglion. No somatotopic organization was observed for CGRP-like immunoreactive perikarya. Small (maximal diameter below 30 microm), medium (maximal diameter between 30 and 50 microm), and large (maximal diameter larger than 50 microm) trigeminal ganglion cells were found among the primary afferent perikarya from extraocular muscles. Among CGRP-like immunoreactive cells, only small and medium cells were observed. Double-labelling experiments indicated the CGRP content of primary afferents of the guinea pig extraocular muscles. The relationship to former morphological categories of ganglion cells is discussed. Primary afferent neurons with CGRP-like immunoreactivity might have efferent functions and might also be involved in inflammatory processes of extraocular muscles.

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Calcitonin gene‐related peptide and corneal innervation: A developmental study in the rat

Jones

Marfurt

1991

J of Comparative Neurology

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The development of calcitonin gene-related peptide-like immunoreactive (CGRP-LI) nerves was studied in neonatal and adult rat corneas stained immunohistochemically according to an avidin biotin peroxidase procedure. At birth, rat corneas already contained dense plexuses of CGRP-LI nerve fibers. Most of the nerves entered the cornea in 12-15 prominent stromal nerve bundles located at regular intervals around the circumference of the cornea. Fibers in these bundles entered the epithelium approximately midway between the limbus and the center of the cornea and supplied extensive central and pericentral areas of the tissue. In addition, smaller numbers of axons entered the cornea individually and in small fascicles located in between the larger bundles and supplied mainly peripheral territory. In the epithelium, the CGRP-LI nerves formed a complex, highly anastomotic meshwork that ramified uniformly throughout central and peripheral areas of the tissues. Fibers in the plexus gave origin to numerous short, stout terminal axons that extended into the adjacent epithelium in all directions with no preferred orientation. During the first week of neonatal life, several changes in CGRP-LI innervation occurred: 1) the innervation density of the central and pericentral cornea increased relative to the peripheral cornea; 2) intraepithelial axons became progressively longer, increased in branching complexity, and oriented preferentially towards the center of the cornea; and 3) a dense innervation of the corneoscleral limbus and, in particular, the branches of the marginal artery, developed. Midway through the second week of life, immature versions of corneal epithelial "leashes," the dominant feature of the adult corneal innervation, were first observed. Over the next 10 days, the leash formations in the central and pericentral cornea gradually became more complex and gave rise to greater numbers of terminal axons, compared to developing leashes in the peripheral cornea. The mature pattern of corneal CGRP-LI innervation was reached on day 21 and remained constant (except for compensatory growth-related elongation of axons) for at least the first 6 months of life. Transection of the ophthalmomaxillary nerve or neonatal administration of the sensory neurotoxin capsaicin resulted in the total loss of CGRP-LI staining from the cornea. In contrast, removal of the superior cervical ganglion had no effect on corneal CGRP-LI staining. The extraordinary density and complexity of the CGRP-LI innervation of the rat cornea demonstrated at all stages of development in this study suggests that these nerves may play important roles in corneal sensory, reflex, and trophic functions.

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Calcitonin gene-related peptide (CGRP) immunoreactive sensory nerves in the human and guinea pig uvea and cornea

Cited by 48 publications

References 25 publications

Peptidergic Innervation of the Rat Cornea

Peptidergic Innervation of the Rat Cornea

Sensory innervation of the guinea pig extraocular muscles: A 1,1?-dioctadecyl-3,3,3?3?-tetramethylindocarbocyanine perchlorate tracing and calcitonin gene-related peptide immunohistochemical study

Calcitonin gene‐related peptide and corneal innervation: A developmental study in the rat

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