Calcitonin gene‐related peptide and pulmonary hypertension in experimental hypoxia

Springall, David R.; Polak, Julia M.

doi:10.1002/ar.1092360113

Cited by 30 publications

(13 citation statements)

References 42 publications

(28 reference statements)

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“…Consequently, it may reasonably be advanced that pulmonary endocrine cell-associated ''esterase'' is tied in with early posttranslational events in the synthesis of endocrine neuropeptides. Knowledge that chromogranin A has been immunolocalized to pulmonary neuroendocrine cells in eight species (Polak, 1993) and that pancreastatin also localizes to pulmonary neuroendocrine cells in three of these species (Seldeslagh and Lauweryns, 1993b) adds grist to the argument.…”

Section: Differentiation and Maturationmentioning

confidence: 99%

Ontogeny of neuroepithelial bodies: Correlations with mitogenesis and innervation

Sorokin¹,

Hoyt²,

Shaffer³

1997

Microsc. Res. Tech.

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This paper summarizes current knowledge and advances speculation about the formation of the neuroendocrine system of mammalian lungs (comprising uninnervated solitary and clustered small-granule cells and innervated neuroepithelial bodies). It relates the initial appearance of neuroendocrine cells to regulation of mitotic activity in the epithelium during the development of the lung and pays special attention to the later in growth of nerves that converts some of them into neuroepithelial bodies, structures considered ideally adapted to function as chemoreceptors. A few original observations from ongoing immunohistochemical, electron microscopic, and analytical studies have been included here and there to point the discussion. The neuroendocrine cells are derived from undifferentiated precursors present in the endodermal pulmonary epithelium. At an early pseudoglandular stage of lung development these precursors begin to differentiate into neuroendocrine small-granule cells, commencing in the larynx and upper trachea, and expanding centrifugally into pulmonary airways almost as rapidly as these are laid down. Subsequently many of the intrapulmonary small-granule cell clusters become innervated. This event, the delayed appearance of small-granule cells synthesizing other than the dominant peptides and amines (calcitonin gene-related peptide and serotonin in rodents, gastrin-releasing peptide and serotonin in human beings), and other regional adjustments yield the population distribution present in the lungs of adults. Neuroendocrine cell precursors normally differentiate into typical serotonin- or peptide-synthesizing small-granule cells without requiring direct contact by nerves, and dissociated cells from a previously innervated population continue to exhibit physiological characteristics of oxygen sensors despite the loss of contact with nerves. Development of the innervation occurs in stages. Small-granule cell clusters are reached first by ganglion cells derived from pulmonary neuroblasts and later on by processes of extrinsic sensory nerves. The latter not only convey information to the central nervous system but also serve in a variety of ways to extend the neuroepithelial bodies' sphere of influence within the lung itself.

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Section: Differentiation and Maturationmentioning

confidence: 99%

Ontogeny of neuroepithelial bodies: Correlations with mitogenesis and innervation

Sorokin¹,

Hoyt²,

Shaffer³

1997

Microsc. Res. Tech.

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“…Intravenous, intracavernosal, or intrathecal administration of exogenous CGRP has been shown to have a beneficial effect in pulmonary hypertension [14][15][16], erectile dysfunction [17,18], and cerebral vasospasm after SAH [19][20][21]. However, the half-life of CGRP in plasma is only 10 minutes, and chronic infusion of CGRP to patients is not feasible [22].…”

mentioning

confidence: 99%

Engineering Ex Vivo–Expanded Marrow Stromal Cells to Secrete Calcitonin Gene–Related Peptide Using Adenoviral Vector

et al. 2004

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IntroductionCalcitonin gene-related peptide (CGRP), a 37-amino-acid neuropeptide derived from alternative splicing of RNA from the calcitonin gene [1], is an important molecule with multiple biological effects, including a potent vasodilator activity [2,3]. CGRP is extensively localized in the perivascular or periadventitia nerves throughout the body, and its release from nerve endings is associated with the dilation of blood vessels [4]. CGRP exerts its vasodilator effect through interaction with CGRP receptors that are present on both endo-

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“…To clarify cellular localization of the amplified products in the tissue, in situ RT-PCR histochemistry is the most suitable method. However, this method is technically more complicated than classical in situ hybridization, and the previous studies with in situ hybridization showed a-CGRP mRNA in rat lungs (Keith et al, 1991;Springall and Polak, 1993). Radioisotope (RI)-labeled probes used in these studies are thought to be more sensitive than digoxygenin (DIG)-labeled probes, but RI-labeled probes were not available in our laboratory.…”

Section: Localization Of Cgrp In Ai Rat Lungs By In Situ Rt-pcr Analymentioning

confidence: 93%

Long-term induction of β-CGRP mRNA in rat lungs by allergic inflammation

et al. 2004

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Calcitonin gene‐related peptide and pulmonary hypertension in experimental hypoxia

Cited by 30 publications

References 42 publications

Ontogeny of neuroepithelial bodies: Correlations with mitogenesis and innervation

Ontogeny of neuroepithelial bodies: Correlations with mitogenesis and innervation

Engineering Ex Vivo–Expanded Marrow Stromal Cells to Secrete Calcitonin Gene–Related Peptide Using Adenoviral Vector

Long-term induction of β-CGRP mRNA in rat lungs by allergic inflammation

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