Calcinated gold nanoparticle arrays for on-chip, multiplexed and matrix-free mass spectrometric analysis of peptides and small molecules

Hinman, Samuel S.; Chen, Chih-Yuan; Duan, Jicheng; Cheng, Quan

doi:10.1039/c5nr06635b

Cited by 38 publications

(30 citation statements)

References 60 publications

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“…The wide use of compatible surface techniques such as SPR and QCM facilitates the characterization of surface interactions in a label-free manner, which is not always possible for molecules that require redox reporters in amperometry. Additional surface-based optical and mass spectrometric measurements will undoubtedly elucidate these processes toward more complete understandings as the integrations become more timesaving and efficient [117, 118]. Taken together, these developments and opportunities are indicative of a bright future for the field.…”

Section: Perspectivementioning

confidence: 99%

Bioinspired assemblies and plasmonic interfaces for electrochemical biosensing

Hinman

Cheng

2016

Journal of Electroanalytical Chemistry

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Electrochemical biosensing represents a collection of techniques that may be utilized for capture and detection of biomolecules in both simple and complex media. While the instrumentation and technological aspects play important roles in detection capabilities, the interfacial design aspects are of equal importance, and often, those inspired by nature produce the best results. This review highlights recent material designs, recognition schemes, and method developments as they relate to targeted electrochemical analysis for biological systems. This includes the design of electrodes functionalized with peptides, proteins, nucleic acids, and lipid membranes, along with nanoparticle mediated signal amplification mechanisms. The topic of hyphenated surface plasmon resonance assays is also discussed, as this technique may be performed concurrently with complementary and/or confirmatory measurements. Together, smart materials and experimental designs will continue to pave the way for complete biomolecular analyses of complex and technically challenging systems.

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Section: Perspectivementioning

confidence: 99%

Bioinspired assemblies and plasmonic interfaces for electrochemical biosensing

Hinman

Cheng

2016

Journal of Electroanalytical Chemistry

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“…Although the exact ionization mechanism remains unclear, the physical properties of nanostructured materials are considered to be associated with energy absorption and transfer that results in ionization of the analytes. Various nanomaterials have been used for LDI-TOF MS, including (1) carbon-based nanomaterials, such as carbon nanotubes, 7 graphene, 8 and diamondlike carbons; 9 (2) semiconductor-based nanomaterials, such as ZnO, SnO, and TiO 2 ; 5,10 (3) metal NPs such as Ag, 11 Pt, 12 and Au NPs; 13 and other materials such as modified iron oxide NPs. 14 Among the various kinds of nanomaterials, gold NPs have frequently been reported as an effective inorganic solid matrix for LDI-TOF MS because of their outstanding physical properties, which are believed to aid the ionization of analytes.…”

Section: Introductionmentioning

confidence: 99%

Gold‐nanoparticle‐coated magnetic beads for concentration and ionization of analytes for laser desorption/ionization mass spectrometry

Kim

Park

Noh

et al. 2019

Rapid Comm Mass Spectrometry

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Rationale Magnetic particles coated with gold nanoparticles (Au NPs) (Au‐MAGs) were developed and used (1) for sample concentration and (2) as a solid matrix for laser adsorption/desorption mass spectrometry (LDI‐MS). Methods The Au‐MAGs were prepared by (1) coating polystyrene on iron oxide NPs (PS‐MNPs), (2) coating poly‐l‐lysine on the PS‐MNPs (PLL‐coated PS‐MNPs), and (3) coating negatively charged Au NPs on the PLL‐coated PS‐MNPs (Au‐MAGs). Results The Au‐MAGs were used to concentrate the target analyte by means of electrostatic interactions between the positively charged GHP9 and the negatively charged Au‐MAGs as well as selective interactions (such as gold–sulfur interactions) between glutathione (GSH) and Au‐MAGs. Then, the concentrated analyte could be ionized for LDI‐MS. Conclusions The Au‐MAGs were demonstrated (1) to concentrate the target analyte in a sample solution, tested by electrostatic interactions and selective interactions between gold and sulfur and (2) to ionize the concentrated analyte for LDI‐MS.

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“…The laser desorption/ionization MS (LDI-MS) techniques could be employed for the analysis the distribution of nanomaterials in the cell and tissue samples 30 , 31 . Recently, nanomaterials and nanostructured substrates prepared from metal, metal oxide, silicon and carbon materials have been widely used as matrices in MS analysis to achieve higher resolution and lower background noise 32 - 39 . However, the LDI-MS techniques for tissue imaging can only detect highly abundant molecules, and therefore analysis of low-abundant proteins remains limited 40 - 43 .…”

Section: Introductionmentioning

confidence: 99%

Satellite-like Gold Nanocomposites for Targeted Mass Spectrometry Imaging of Tumor Tissues

Tseng¹,

Harroun²,

Wu³

et al. 2017

Nanotheranostics

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We have developed a simple, rapid, high-throughput cancer diagnosis system using functional nanoparticles (NPs) consisting of poly(catechin) capped-gold NPs (Au@PC NPs) and smaller nucleolin-binding aptamer (AS1411) conjugated gold NPs (AS1411-Au NPs). The AS1411-Au NPs/Au@PC NP is used as a targeting agent in laser desorption/ionization mass spectrometry (LDI-MS)-based tumor tissue imaging. Self-assembled core-shell Au@PC NPs are synthesized by a simple reaction of tetrachloroaurate(III) with catechin. Au@PC NPs with a well-defined and dense poly(catechin) shell (~40-60 nm) on the surface of each Au core (~60-80 nm) are obtained through careful control of the ratio of catechin to gold ions, as well as the pH of the reaction solution. Furthermore, we have shown that AS1411-conjugated Au NPs (13-nm) self-assembled on Au@PC NP can from a satellite-like gold nanocomposite. The high density of AS1411-Au NPs on the surface of Au@PC NP enhances multivalent binding with nucleolin molecules on tumor cell membranes. We have employed LDI-MS to detect AS1411-Au NPs/Au@PC NPs labeled nucleolin-overexpressing MCF-7 breast cancer cells through the monitoring of Au cluster ions ([Aun]+; 1 ≤ n ≤ 3). The ultrahigh signal amplification from Au NPs through the formation of a huge number of [Aun]+ ions results in a sensing platform with a limit of detection of 100 MCF-7 cells mL-1. Further, we have applied the satellite-like AS1411-Au NPs/Au@PC NP nanocomposite as a labeling agent for tumor tissue imaging by LDI-MS. Our nanocomposite-assisted LDI-MS imaging platform can be extended for simultaneous analysis of different tumor markers on cell membranes when using different ligand-modified metal nanoparticles.

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Calcinated gold nanoparticle arrays for on-chip, multiplexed and matrix-free mass spectrometric analysis of peptides and small molecules

Cited by 38 publications

References 60 publications

Bioinspired assemblies and plasmonic interfaces for electrochemical biosensing

Bioinspired assemblies and plasmonic interfaces for electrochemical biosensing

Gold‐nanoparticle‐coated magnetic beads for concentration and ionization of analytes for laser desorption/ionization mass spectrometry

Satellite-like Gold Nanocomposites for Targeted Mass Spectrometry Imaging of Tumor Tissues

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