Calcimycin-induced lens epithelial cell apoptosis contributes to cataract formation

Li, Wancheng; Kuszak, J.R.; Wang, Guo‐Ming; Wu, Zaiqi; Spector, Abraham

doi:10.1016/s0014-4835(95)80062-x

Cited by 64 publications

(50 citation statements)

References 36 publications

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“…The lenses were incubated with calcimycin to induce apoptosis, as previously reported (32). Incubation with calcimycin induced apoptosis of epithelial cells, as observed by TUNEL staining (Fig.…”

Section: Resultsmentioning

confidence: 59%

Chaperone Peptides of α-Crystallin Inhibit Epithelial Cell Apoptosis, Protein Insolubilization, and Opacification in Experimental Cataracts

Nahomi

Wang

Raghavan

et al. 2013

Journal of Biological Chemistry

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Section: Resultsmentioning

confidence: 59%

Chaperone Peptides of α-Crystallin Inhibit Epithelial Cell Apoptosis, Protein Insolubilization, and Opacification in Experimental Cataracts

Nahomi

Wang

Raghavan

et al. 2013

Journal of Biological Chemistry

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“…[4][5][6] However, a question arises as to whether apoptosis is in- duced when cataract occurs by aging or by external stress, such as diabetes, and this issue is still being debated. [8][9][10][11][12] In this study, to obtain information on this issue, lens epithelium was examined by the TUNEL method to investigate the relationship between apoptosis and cataract.…”

Section: Discussionmentioning

confidence: 99%

“…Apoptosis during the development/morphogenensis in the crystalline lens has been established, but there are many unclear points and controversies regarding the induction of apoptosis in acquired cataract. [4][5][6][7][8][9][10][11][12] In this study, we investigated the presence of apoptosis in the lens epithelial cells of humans and in a rat diabetic cataract model using the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end labeling (TUNEL) method.…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical Study of Apoptosis of Lens Epithelial Cells in Human and Diabetic Rat Cataracts

Takamura

2001

Japanese Journal of Ophthalmology

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Purpose: To evaluate apoptosis of lens epithelial cells by immunohistochemical methods. Methods:We performed terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) assays on capsulotomy specimens (53 patients, 68 eyes) from patients who had undergone cataract surgery, and on the epithelium of diabetic cataracts in rats (72 rats, 144 eyes). An animal model of diabetic cataracts was prepared by injection of streptozotocin in 3-week-old rats. The specimens of rats were also examined using the proliferating cell nuclear antigen (PCNA) immunohistochemical staining method.Results: Although some TUNEL-positive cells were detected in capsulotomy specimens, we recognized little correlation between their distribution and the morphological classification of the cataracts. In the animal model of diabetic cataracts, TUNEL-positive cells were seen around the regions of accumulated epithelial cells. In the accumulated regions, PCNAlabeled cells undergoing DNA synthesis were also detected. Conclusions

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“…[1][2][3] In lens system, our previous work [4][5][6] has suggested that induced lens epithelial cell apoptosis appears to be a common cellular mechanism mediating stress-induced, noncongenital cataractogenesis. Subsequently, several laboratories have reported that lens epithelial cell apoptosis is indeed actively involved in lens pathogenesis as demonstrated from in vivo animal model studies.…”

Section: Introductionmentioning

confidence: 99%

Human αA- and αB-crystallins bind to Bax and Bcl-XS to sequester their translocation during staurosporine-induced apoptosis

et al. 2004

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aA-and aB-crystallins are distinct antiapoptotic regulators. Regarding the antiapoptotic mechanisms, we have recently demonstrated that aB-crystallin interacts with the procaspase-3 and partially processed procaspase-3 to repress caspase-3 activation. Here, we demonstrate that human aA-and aBcrystallins prevent staurosporine-induced apoptosis through interactions with members of the Bcl-2 family. Using GST pulldown assays and coimmunoprecipitations, we demonstrated that a-crystallins bind to Bax and Bcl-X S both in vitro and in vivo. Human aA-and aB-crystallins display similar affinity to both proapoptotic regulators, and so are true with their antiapoptotic ability tested in human lens epithelial cells, human retina pigment epithelial cells (ARPE-19) and rat embryonic myocardium cells (H9c2) under treatment of staurosporine, etoposide or sorbitol. Two prominent mutants, R116C in aA-crystallin and R120G, in aB-crystallin display much weaker affinity to Bax and Bcl-X S . Through the interaction, a-crystallins prevent the translocation of Bax and Bcl-X S from cytosol into mitochondria during staurosporine-induced apoptosis. As a result, a-crystallins preserve the integrity of mitochondria, restrict release of cytochrome c, repress activation of caspase-3 and block degradation of PARP. Thus, our results demonstrate a novel antiapoptotic mechanism for a-crystallins. Keywords: aA-crystallin; R116C; aB-crystallin; R120G; Bax; Bcl-X S Abbreviations: a-crystallin, alpha-crystallin; GFP, green fluorescence protein; HaA, human aA-crystallin; HaB, human aB-crystallin; GFP-HaA, green fluorescence and human aAcrystallin fusion protein; GFP-HaB, green fluorescence and human aB-crystallin fusion protein; MEM, Eagle's minimal essential medium; PAGE, polyacrylamide gel electrophoresis; PBS, phosphate-buffered saline; PMSF, phenylmethylsufonyl fluoride; HLE, human lens epithelial cells; SDS, sodium dodecylsulfate; TBS, tris-buffered saline; TBS-T, tris-buffered saline with tween-20.

show abstract

Calcimycin-induced lens epithelial cell apoptosis contributes to cataract formation

Cited by 64 publications

References 36 publications

Chaperone Peptides of α-Crystallin Inhibit Epithelial Cell Apoptosis, Protein Insolubilization, and Opacification in Experimental Cataracts

Chaperone Peptides of α-Crystallin Inhibit Epithelial Cell Apoptosis, Protein Insolubilization, and Opacification in Experimental Cataracts

Immunohistochemical Study of Apoptosis of Lens Epithelial Cells in Human and Diabetic Rat Cataracts

Human αA- and αB-crystallins bind to Bax and Bcl-XS to sequester their translocation during staurosporine-induced apoptosis

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