Calcifying Cystic Odontogenic Tumour: immunohistochemical expression of matrix metalloproteinases, their inhibitors (<scp>TIMP</scp>s and <scp>RECK</scp>) and inducer (<scp>EMMPRIN</scp>)

Prosdócimi, Fábio César; Rodini, Camila Oliveira; Sogayar, Mari Cleide; Sousa, Suzana Cantanhede Orsini Machado de; Xavier, Flávia Caló Aquino; Paiva, Katiúcia Batista Silva

doi:10.1111/jop.12154

Cited by 7 publications

(4 citation statements)

References 51 publications

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“…A low level of expression increased the rates of tongue cancer invasion and metastasis, and the regulation may be related to an inhibitory mechanism of RECK on MMP-2 and MMP-9 expression (34). RECK shows an obviously negative correlation with MMP-2 and MMP-9 expression, suggesting that in the process of tumor invasion and metastasis, the expression of RECK is downregulated, limiting the inhibition of RECK on MMP-2 and MMP-9, thus achieving invasion and metastasis of tumor tissues (35). The present study first demonstrated that icariin increased the RECK protein expression level in ovarian cancer A2780 cells.…”

Section: Discussionsupporting

confidence: 55%

Icariin regulates the proliferation and apoptosis of human ovarian cancer cells through microRNA-21 by targeting PTEN, RECK and Bcl-2

Jiang

Zhao

2015

Oncology Reports

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Icariin is the main active ingredient found in the traditional Chinese medicinal plant Epimedium, and exhibits various pharmacological effects such as enhanced immune function, anticancer activity, improved cardiovascular function and endocrine adjustment. However, the effect of icariin on ovarian cancer and the related mechanism have never been investigated. In the present study, we aimed to verify whether icariin inhibits the proliferation and increases the apoptosis of human ovarian cancer cells, and its molecular mechanism in order to establish an association and identify potential therapeutic targets. In the present study, ovarian cancer A2780 cells were treated with various concentrations of icariin, and the cell viability was evaluated by 3,3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Flow cytometry and caspase-3 colorimetric assay were performed to observe apoptotic changes in the A2780 cells. qPCR analysis was used to analyze miR-21 expression in the A2780 cells. Western blot analysis was used to assess PTEN, RECK and Bcl-2 protein expression. Transfection of microRNA-21 (miR-21) and anti-miR-21 was used to investigate expression of its target genes associated with cell proliferation and apoptosis. Icariin concomitantly suppressed cell proliferation, accelerated apoptosis and increased caspase-3 activity in the A2780 cells. In the ovarian cancer A2780 cells, icariin substantially decreased the miR-21 expression level, increased PTEN and RECK protein expression levels and decreased the Bcl-2 protein expression level. Notably, miR-21 regulated the potential anticancer effects of icariin on cell proliferation and apoptosis by targeting PTEN, RECK and Bcl-2 in the ovarian cancer A2780 cells. Our results demonstrated that icariin is an excellent candidate antitumor agent which exhibits an anticancer curative effect on ovarian cancer cells. miR-21 and its target genes may play a vital role in the molecular mechanism of the anticancer effects of icariin.

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Section: Discussionsupporting

confidence: 55%

Icariin regulates the proliferation and apoptosis of human ovarian cancer cells through microRNA-21 by targeting PTEN, RECK and Bcl-2

Jiang

Zhao

2015

Oncology Reports

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“…In certain localities they were grouped forming large areas of eosinophilic and acellular material. Although the literature says that it is possible to observe mineralized material similar to dentin 14 , in our case, the mineral product was in form of a dystrophic mineralization.…”

Section: Discussioncontrasting

confidence: 53%

Calcifying cystic odontogenic tumor of maxila

<¹,

Oliveira²,

Cadavid³

et al. 2017

J Oral Diag

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The calcifying cystic odontogenic tumor (CCOT) is an uncommon pathology, representing about 2% of all odontogenic cysts and tumors. Generally, is presented as slow growth, asymptomatic, without predilection of gender or location. The most common radiographic presentation is radiolucent image with radiopaque areas, especially in the lesion periphery, unilocular, expansive and well-defined margin. The purpose of this report is to describe the case of a 16-year-old boy with a painless and progressive increase of volume on the face, which started 7 months ago. In oroscopy, we observed an expansive increase of volume in right maxilla, extending from premolars to molars on the same side. In computed tomography, it was possible to observe an unilocular, expansive image in right maxilla. After incisional biopsy, the histopathological analysis evidenced epithelial neoplasia of odontogenic origin that presents cystic portions and solid portions. In the cystic portions, there is an epithelial lining which basal cells are cuboidal or cylindrical arrangement in palisade, presence of ghost cells and dystrophic mineralization, confirming the diagnosis of calcifying cystic odontogenic tumor. Excision and curettage were performed under general anesthesia. The excised tumor was analyzed, confirming the diagnosis of CCOT and the patient has been in clinical and radiographic follow-up for a period of 5 years, without signs of relapses.

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“…[4][5][6] During AME progression, mitogens are randomly released and promote the secretion of matrix metalloproteinases (MMPs) that induce localized proteolysis of the extracellular matrix. 4,5,7 Recent studies have revealed that localized proteolysis is mediated by invadopodia, 8,9 which are finger-like cellular protrusions that have intrinsic proteolytic activity and are associated with early tumour invasion and activation of MMPs. 10,11 Cortactin and membrane type 1 MMP (MT1-MMP) have been described in AME.…”

Section: Introductionmentioning

confidence: 99%

Role of hypoxia‐related proteins in invasion of ameloblastoma cells: crosstalk between NOTCH1, hypoxia‐inducible factor 1α, a disintegrin and metalloproteinase 12, and heparin‐binding epidermal growth factor

et al. 2016

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Calcifying Cystic Odontogenic Tumour: immunohistochemical expression of matrix metalloproteinases, their inhibitors (TIMPs and RECK) and inducer (EMMPRIN)

Abstract: We concluded that these proteins/enzymes are differentially expressed in both epithelium and stroma of CCOT, suggesting an imbalance between MMPs and their inducer/inhibitors may contribute on the tumour behaviour.

Cited by 7 publications

References 51 publications

Icariin regulates the proliferation and apoptosis of human ovarian cancer cells through microRNA-21 by targeting PTEN, RECK and Bcl-2

Icariin regulates the proliferation and apoptosis of human ovarian cancer cells through microRNA-21 by targeting PTEN, RECK and Bcl-2

Calcifying cystic odontogenic tumor of maxila

Role of hypoxia‐related proteins in invasion of ameloblastoma cells: crosstalk between NOTCH1, hypoxia‐inducible factor 1α, a disintegrin and metalloproteinase 12, and heparin‐binding epidermal growth factor

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